GST Activity Assay Kit (Fluorometric) (ab65325)
- Product nameGST Activity Assay Kit (Fluorometric)See all GST kits ...
- Tests100 x 1 assay
- Sample typeUrine, Serum, Plasma, Other biological fluids, Tissue Extracts, Cell Lysate, Cell culture media
- Assay typeEnzyme activity
- Sensitivity< 0.5 mU/well
- Assay time2h 00m
- Product overview
Abcam's GST Activity Assay Kit (Fluorometric) provides a simple, fluorescence-based in vitro assay for detecting the GST activity using fluorescence plate reader. The assay utilizes monochlorobimane (MCB), a dye that reacts with glutathione. The free form of MCB is almost nonfluorescent, whereas the dye fluoresces blue (Ex/Em = 380/461 nm) when reacts with glutathione. GST catalyzes the MCB-glutathione reactions and the fluorescence levels are proportionally to the amounts of GST presence in the reaction. Thus, the GST level in samples can be easily detected using a fluorometer or a 96-well fluorometric plate reader. The kit can detect GST activity in crude cell lysate or purified protein fraction, and also quantitate GST-tagged fusion protein.
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Glutathione S-transferase (GST) is a family of enzymes that play an important role in detoxification of xenobiotics. GST catalyzes the formation of the thiol group of glutathione to electrophilic xenobiotics. It utilizes glutathione to scavenge potentially toxic compounds including those produced as a result of oxidative stress and is part of the defense mechanism against the mutagenic, carcinogenic and toxic effects of such compounds.
- Tested applicationsFunctional Studies more details
- Storage instructionsStore at -20°C. Please refer to protocols.
Components Identifier 100 tests Glutathione (lyophilized) Yellow 2 vials GST Assay Buffer 1 x 10ml GST Sample Buffer WM 1 x 25ml GST Standard (5 mU/µl) Green 1 x 50µl Monochlorobimane Substrate Red 1 x 200µl
- Research Areas
- Glutathione S transferase A1Glutathione S transferase A2GST class alpha 1
- GST class alpha 2GST epsilonGST gammaGSTA1GSTA2GTH1GTH2HA subunit 1HA subunit 2
Our Abpromise guarantee covers the use of ab65325 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
GST Activity Assay Kit (Fluorometric) images
Standard Calibration Curve of GST Measured by Fluorometry. Various amounts of standard GST were incubated with GSH and MCB according to the kit instructions. Fluorescence was measured at ex/em 380/460 nm.
References for GST Activity Assay Kit (Fluorometric) (ab65325)
ab65325 has not yet been referenced specifically in any publications.