![Western blot - GST antibody [3G10/1B3] (ab92) image](#ab92_1.jpg)
![Western blot - GST antibody [3G10/1B3] (ab92) image](#ab92_2.jpg)
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Products:Tags & Cell Markers >> Fusion / Marker Proteins >> GST
Overview
Product name
Anti-GST antibody [3G10/1B3]
See all GST products (49) ...
Description
Mouse monoclonal [3G10/1B3] to GST
Specificity
Recognizes Glutathione S-Transferase (GST), a 26-kDa protein encoded by the parasitic helminth Schistosoma japonicum.
Tested applications
WB, IPmore details
Cross reactivity
Reacts with
Mouse, Human
Immunogen
Recombinant full length protein corresponding to amino acids 1-425 of GST (from Schistosoma japonicum, expressed in E. coli).
Positive control
Hela cell whole cell lysate
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
PBS pH 7.4 w/o preservative
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Purification notes
Purified from hybridoma cell culture supernatant by Protein G chromatography to at least 95% homogeneity as determined by SDS-PAGE.
Clonality
Monoclonal
Clone number
3G10/1B3
Myeloma
NS1
Isotype
IgG1
Light chain type
kappa
Research areas
Applications
Show applications key
Our Abpromise guarantee covers the use of ab92 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use a concentration of 0.5 - 2 µg/ml.Detects a band of approximately 30 kDa (predicted molecular weight: 26 kDa).
IP: Use at an assay dependent dilution.
Target
Relevance
GST (Glutathione S-Transferase) is a 26kDa protein encoded by the parasitic helminth Schistosoma japonicum and widely used in the pGEX family of GST plasmid expression vectors as a fusion protein with foreign proteins.
Alternative names
- Glutathione S Transferase antibody
- Glutathione S transferase Mu 1 antibody
- GST 26 antibody
see all
Anti-GST antibody [3G10/1B3] images:
Western blot - GST antibody [3G10/1B3] (ab92)
![Western blot - GST antibody [3G10/1B3] (ab92)](/ps/datasheet/Images/0/ab92/ab92_1.jpg)
Anti-GST antibody [3G10/1B3] (ab92) + HeLa whole cell lysate
Predicted band size : 26 kDa
Observed band size : 30 kDa (why is the actual band size different from the predicted?)
Western blot - GST antibody [3G10/1B3] (ab92)
![Western blot - GST antibody [3G10/1B3] (ab92)](/ps/datasheet/Images/0/ab92/ab92_2.jpg)
All lanes : Anti-GST antibody [3G10/1B3] (ab92)
Lane 1 : Total lysate
Lane 2 : Cytoplasmic fraction
Lane 3 : Membrane fraction
Lane 4 : Nuclear fraction
Predicted band size : 26 kDa
Detection of human GST in cytoplasmic fraction by anti-GST 3G10 monoclonal antibody (ab92) in western blot experiment. p84 is also shown indicating the nuclear fraction using anti-p84 monoclonal antibody (ab487).
References for Anti-GST antibody [3G10/1B3] (ab92)
This product has been referenced in:
- Wang Yet al. Nogo-66 inhibits the dye-coupling of astrocytic gap junctions in vitro. Neurochem Res 36:1129-34 (2011). WB.Read more (PubMed: 21461775) »
- Revenko ASet al. Chromatin loading of E2F-MLL complex by cancer-associated coregulator ANCCA via reading a specific histone mark. Mol Cell Biol 30:5260-72 (2010).Read more (PubMed: 20855524) »
See all 6 publications for this product
Publishing research using ab92? Please let us know so that we can cite the reference in this datasheet
![Anti-GST antibody [3G10/1B3] for Western blot in Human (92)](/ps/datasheet/images/0/ab92/GST-Primary-antibodies-ab92-5.jpg)
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"