GST antibody [3G10/1B3] (ab92)

Product Name 

GST antibody [3G10/1B3]

Product type 

Primary antibodies

Description 

Mouse monoclonal [3G10/1B3] to GST

Immunogen 

Recombinant full length protein corresponding to amino acids 1-425 of GST (from Schistosoma japonicum, expressed in E. coli).

Reacts with 
(species key)

Hu, Ms

Specificity 

Recognizes Glutathione S-Transferase (GST), a 26-kDa protein encoded by the parasitic helminth Schistosoma japonicum.

Tested applications 
(see key)

IP, WB

Abreviews 

Customer reviews (see key)

Read about Abreviews

Application notes 
(see key)

Recommended dilutions
IP: Use at an assay dependent dilution.
WB: Use at a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 30 kDa (predicted molecular weight: 26 kDa).


Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Positive control 
(see definition)

Hela cell whole cell lysate

Research areas 

Tags & Cell Markers >> Fusion / Marker Proteins >> GST

Relevance 

GST (Glutathione S-Transferase) is a 26kDa protein encoded by the parasitic helminth Schistosoma japonicum and widely used in the pGEX family of GST plasmid expression vectors as a fusion protein with foreign proteins.

Raised in 

Mouse

Clonality 

Monoclonal

Clone number 

3G10/1B3

Myeloma 

NS1

Isotype 

IgG1

Light chain type 

kappa

Purity 

Immunogen affinity purified

Storage buffer 

PBS pH 7.4 w/o preservative

Purification notes 

Purified from hybridoma cell culture supernatant by Protein G chromatography to at least 95% homogeneity as determined by SDS-PAGE.

Form 

Liquid

Concentration 

2.000 mg/ml

Storage instructions 

Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

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References for GST antibody [3G10/1B3] (ab92)

  This product has been used in: (two most recent references)

Knauer SK et al. Translocation biosensors to study signal-specific nucleo-cytoplasmic transport, protease activity and protein-protein interactions. Traffic 6:594-606 (2005). PubMed: 15941410

Luque CM et al. An alternative domain containing a leucine-rich sequence regulates nuclear cytoplasmic localization of protein 4.1R. J Biol Chem 278:2686-91 (2003). PubMed: 12427749

See all 3 publication references for this product.


If you publish research using ab92 please let us know so that we can cite the reference on this datasheet.

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