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Anti-GST antibody [3G10/1B3]
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Mouse monoclonal [3G10/1B3] to GST
Recognizes Glutathione S-Transferase (GST), a 26-kDa protein encoded by the parasitic helminth Schistosoma japonicum.
WB, IPmore details
Reacts with
Mouse, Human
Recombinant full length protein corresponding to amino acids 1-425 of GST (from Schistosoma japonicum, expressed in E. coli).
Hela cell whole cell lysate
Liquid
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
PBS pH 7.4 w/o preservative
Concentration information loading...
Immunogen affinity purified
Purified from hybridoma cell culture supernatant by Protein G chromatography to at least 95% homogeneity as determined by SDS-PAGE.
Monoclonal
3G10/1B3
NS1
IgG1
kappa
Our Abpromise guarantee covers the use of ab92 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use a concentration of 0.5 - 2 µg/ml.Detects a band of approximately 30 kDa (predicted molecular weight: 26 kDa).
IP: Use at an assay dependent dilution.
GST (Glutathione S-Transferase) is a 26kDa protein encoded by the parasitic helminth Schistosoma japonicum and widely used in the pGEX family of GST plasmid expression vectors as a fusion protein with foreign proteins.
Western blot - GST antibody [3G10/1B3] (ab92)
![Western blot - GST antibody [3G10/1B3] (ab92)](/ps/datasheet/Images/0/ab92/ab92_1.jpg)
Anti-GST antibody [3G10/1B3] (ab92) + HeLa whole cell lysate
Predicted band size : 26 kDa
Observed band size : 30 kDa (why is the actual band size different from the predicted?)
Western blot - GST antibody [3G10/1B3] (ab92)
![Western blot - GST antibody [3G10/1B3] (ab92)](/ps/datasheet/Images/0/ab92/ab92_2.jpg)
All lanes : Anti-GST antibody [3G10/1B3] (ab92)
Lane 1 : Total lysate
Lane 2 : Cytoplasmic fraction
Lane 3 : Membrane fraction
Lane 4 : Nuclear fraction
Predicted band size : 26 kDa
Detection of human GST in cytoplasmic fraction by anti-GST 3G10 monoclonal antibody (ab92) in western blot experiment. p84 is also shown indicating the nuclear fraction using anti-p84 monoclonal antibody (ab487).
This product has been referenced in:
See all 6 publications for this product
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![Western blot - GST antibody [3G10/1B3] (ab92)](/ps/datasheet/Images/0/ab92/ab92_1.jpg)
Anti-GST antibody [3G10/1B3] (ab92) + HeLa whole cell lysate
Predicted band size : 26 kDa
Observed band size : 30 kDa (why is the actual band size different from the predicted?)
![Western blot - GST antibody [3G10/1B3] (ab92)](/ps/datasheet/Images/0/ab92/ab92_2.jpg)
All lanes : Anti-GST antibody [3G10/1B3] (ab92)
Lane 1 : Total lysate
Lane 2 : Cytoplasmic fraction
Lane 3 : Membrane fraction
Lane 4 : Nuclear fraction
Predicted band size : 26 kDa
Detection of human GST in cytoplasmic fraction by anti-GST 3G10 monoclonal antibody (ab92) in western blot experiment. p84 is also shown indicating the nuclear fraction using anti-p84 monoclonal antibody (ab487).
1
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