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Western blot - GST antibody [3G10/1B3] (ab92)
![Western blot - GST antibody [3G10/1B3] (ab92)](/ps/datasheet/images/ab92_1.jpg)
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Western blot - GST antibody [3G10/1B3] (ab92)
![Western blot - GST antibody [3G10/1B3] (ab92)](/ps/datasheet/images/ab92_2.jpg)
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Product Name
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GST antibody [3G10/1B3]
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See all GST antibodies (33)...
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Product type
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Primary antibodies
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Description
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Mouse monoclonal [3G10/1B3] to GST
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Immunogen
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Recombinant full length protein corresponding to amino acids 1-425 of GST (from Schistosoma japonicum, expressed in E. coli).
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Reacts with
(species key)
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Hu, Ms
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Specificity
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Recognizes Glutathione S-Transferase (GST), a 26-kDa protein encoded by the parasitic helminth Schistosoma japonicum.
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Tested applications
(see key)
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IP, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions IP: Use at an assay dependent dilution. WB: Use at a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 30 kDa (predicted molecular weight: 26 kDa).
Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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Hela cell whole cell lysate
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Research areas
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Tags & Cell Markers >> Fusion / Marker Proteins >> GST
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Relevance
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GST (Glutathione S-Transferase) is a 26kDa protein encoded by the parasitic helminth Schistosoma japonicum and widely used in the pGEX family of GST plasmid expression vectors as a fusion protein with foreign proteins.
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Raised in
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Mouse
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Clonality
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Monoclonal
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Clone number
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3G10/1B3
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Myeloma
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NS1
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Isotype
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IgG1
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Light chain type
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kappa
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Purity
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Immunogen affinity purified
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Storage buffer
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PBS pH 7.4 w/o preservative
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Purification notes
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Purified from hybridoma cell culture supernatant by Protein G chromatography to at least 95% homogeneity as determined by SDS-PAGE.
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Form
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Liquid
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Concentration
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2.000 mg/ml
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this GST antibody [3G10/1B3] is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for GST antibody [3G10/1B3] images, references, products related to ab92 and other tools.
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GST antibody [3G10/1B3] images:
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Western blot - GST antibody [3G10/1B3] (ab92)
All lanes : GST antibody [3G10/1B3] (ab92)
Lane 1 : Total lysate Lane 2 : Cytoplasmic fraction Lane 3 : Membrane fraction Lane 4 : Nuclear fraction
Predicted band size : 26 kDa
Detection of human GST in cytoplasmic fraction by anti-GST 3G10 monoclonal antibody (ab92) in western blot experiment. p84 is also shown indicating the nuclear fraction using anti-p84 monoclonal antibody (ab487).
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References for GST antibody [3G10/1B3] (ab92)
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This product has been used in: (two most recent references)
Knauer SK et al. Translocation biosensors to study signal-specific nucleo-cytoplasmic transport, protease activity and protein-protein interactions. Traffic 6:594-606 (2005). PubMed: 15941410
Luque CM et al. An alternative domain containing a leucine-rich sequence regulates nuclear cytoplasmic localization of protein 4.1R. J Biol Chem 278:2686-91 (2003). PubMed: 12427749
See all 3 publication references for this product.
If you publish research using ab92 please let us know so that we can cite the reference on this datasheet.
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Search PubMed (MEDLINE) for references to GST
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GST antibody [3G10/1B3] - more information
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"