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Products:Tags & Cell Markers >> Fusion / Marker Proteins >> GST
Overview
Product name
Anti-GST antibody
See all GST products (49) ...
Description
Goat polyclonal to GST
Tested applications
WB, IP, ELISA, Conjugation, Dot Blotmore details
Cross reactivity
Reacts with Schistosoma japonicum. Not yet tested on other species.
Immunogen
Glutathione-S-Transferase [Schistosoma japonicum]
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.01% Sodium Azide
Constituents: 15mg/ml BSA, 0.25M Sodium chloride, 0.01M Sodium phosphate, pH 7.6
Concentration
Concentration information loading...
Purity
IgG fraction
Purification notes
This product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.
Clonality
Polyclonal
Isotype
IgG
Research areas
-
Western blot - GST antibody (ab6613)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab6613 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use at an assay dependent concentration.
IP: Use at an assay dependent concentration.
ELISA: Use at an assay dependent concentration.
Conjugation: Use at an assay dependent concentration.
Dot: Use at an assay dependent concentration.
Target
Relevance
GST (Glutathione S-Transferase) is a 26kDa protein encoded by the parasitic helminth Schistosoma japonicum and widely used in the pGEX family of GST plasmid expression vectors as a fusion protein with foreign proteins.
Alternative names
- Glutathione S Transferase antibody
- Glutathione S transferase Mu 1 antibody
- GST 26 antibody
see all
Anti-GST antibody images:
Western blot - GST antibody (ab6613)
Anti-GST antibody (ab6613) at 1/5000 dilution + purified GST recombinant protein at 10 µg
Secondary
HRP conjugated donkey anti-goat antibody at 1/2000 dilution
developed using the ECL technique
Performed under reducing conditions.
Observed band size : 30 kDa (why is the actual band size different from the predicted?)
Exposure time : 30 seconds
This image is courtesy of an anonymous Abreview
References for Anti-GST antibody (ab6613)
This product has been referenced in:
- Colnaghi R & Wheatley SP Liaisons between Survivin and Plk1 during Cell Division and Cell Death. J Biol Chem 285:22592-604 (2010). WB.Read more (PubMed: 20427271) »
- Maeda Yet al. PARP-2 interacts with TTF-1 and regulates expression of surfactant protein-B. J Biol Chem 281:9600-6 (2006). WB.Read more (PubMed: 16461352) »
See all 5 publications for this product
Publishing research using ab6613? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"