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Products:Tags & Cell Markers >> Fusion / Marker Proteins >> GST
Overview
Product name
Anti-GST antibody
See all GST products (49) ...
Description
Rabbit polyclonal to GST
Tested applications
ELISA, IF, IP, WB, ICC/IFmore details
Cross reactivity
Reacts with Schistosoma japonicum. Not yet tested on other species.
Immunogen
Rabbits were immunized with Glutathione-S-transferase (GST) from Schistosoma japonicum.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Tris pH7, 50% glycerol
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Purification notes
Affinity purified against bacterially expressed GST
Clonality
Polyclonal
Isotype
IgG
Research areas
-
Immunocytochemistry/ Immunofluorescence - GST antibody (ab9085)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab9085 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ELISA
ELISA: 1/1000ELISA: 1/1000
IF: 1/500
IP: Use at an assay dependent dilution.
WB: 1/500
ICC/IF: Use at an assay dependent dilution. (PubMed: 21242312)
Target
Relevance
GST (Glutathione S-Transferase) is a 26kDa protein encoded by the parasitic helminth Schistosoma japonicum and widely used in the pGEX family of GST plasmid expression vectors as a fusion protein with foreign proteins.
Alternative names
- Glutathione S Transferase antibody
- Glutathione S transferase Mu 1 antibody
- GST 26 antibody
see all
Anti-GST antibody images:
Immunocytochemistry/ Immunofluorescence - GST antibody (ab9085)
ab9085 staining GST in wild-type (GST–NUAK2-wt) expressing HeLa cells by Immunocytochemistry/ Immunofluorescence. Cells were treated with 10µM Y27632 inhibitor for 30 minutes before fixation.
Image from Vallenius T et al, J Cell Sci. 2011 Feb 1;124(Pt 3):384-93, Fig 4. DOI : 10.1242/jcs.072660
References for Anti-GST antibody (ab9085)
This product has been referenced in:
- Vallenius Tet al. An association between NUAK2 and MRIP reveals a novel mechanism for regulation of actin stress fibers. J Cell Sci 124:384-93 (2011). ICC/IF; Human.Read more (PubMed: 21242312) »
- Maison Cet al. SUMOylation promotes de novo targeting of HP1a to pericentric heterochromatin. Nat Genet : (2011). WB.Read more (PubMed: 21317888) »
See all 11 publications for this product
Publishing research using ab9085? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"