Overview
- Product nameGST tag ELISA Kit
- Precision
Intra-assay Sample n Mean SD CV% Overall 8.5% - Tests1 x 96 well plate
- Sample typeCell Lysate
- Assay typeSandwich
- Sensitivity1 ng/ml
- Product overview
Gluthathione S-transferase (GST) is a 26 kDa protein typically used as a fusion partner of recombinant proteins to increase the level of protein expression in bacterial systems, enhance solubility, protect from proteolysis, improve folding and allow purification by affinity chromatography on gluthathione resin.
ab126581 GST tag ELISA Kit is an enzyme immunoassay developed for the sensitive detection and quantitation of GST or GST fusion proteins in cells samples or purified tagged proteins. The quantity of GST can be determined in lysates or intermediate purification fractions by comparing their absorbance with that of a known recombinant GST standard curve. The kit has a detection sensitivity limit of 1 ng/mL of GST. Each kit provides sufficient reagents to perform up to 96 assays including standard curve and GST fusion proteins.
- Notes
Typical working ranges
GST (pure protein) 1 – 200 ng/mL
GST fusion protein 6 – 400ng/mL
- Tested applicationsELISA more details
Properties
- Storage instructionsStore at +4°C. Please refer to protocols.
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Components 1 x 96 tests 10X Blocking Buffer 1 x 6ml 10X GST Detector Antibody 1 x 700µl 10X HRP Label 1 x 1ml 20X Buffer 1 x 20ml Extraction Buffer 1 x 15ml GST Microplate (12 x 8 antibody coated well strips) 1 unit GST Standard 1 vial TMB Development Solution 1 x 6ml - Research Areas
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Alternative names
- Glutathione S-transferase A1Glutathione S-transferase alpha 1GST class-alpha member 1
- GST HA subunit 1GST-epsilonGST2GSTA1-1GTH1
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Applications
Our Abpromise guarantee covers the use of ab126581 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Notes |
|---|---|
| ELISA | ELISA |
GST tag ELISA Kit images
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ELISA - GST tag ELISA Kit (ab126581)Example standard curve. GST standard protein was serially diluted from 400 ng/mL to 6 ng/mL. Background was subtracted from data points prior to log transformation and curve fitting (log-agonist vs. response – variable slope). Sensitivity was calculated mathematically to be as low as 1 ng/mL. -
ELISA - GST tag ELISA Kit (ab126581)Dynamic Range of a recombinant protein tagged to GST. Recombinant full length protein (Human) p53 with N terminal GST tag (ab43615) was tested in a dilution series from 400 ng/mL to 12 ng/mL using the GST ELISA kit. Background was subtracted from data points prior to log transformation and curve fitting (log-agonist vs. response – variable slope). -
Western blot - GST tag ELISA Kit (ab126581)All lanes : GST tag ELISA Kit (ab126581)
Lane 1 : GST recombinant protein (ab89494)
Lane 2 : p53 tagged to GST protein (ab43615) -
Western blot - GST tag ELISA Kit (ab126581)All lanes : Anti-GST antibody (ab19256)
Lane 1 : GST recombinant protein (ab89494)
Lane 2 : p53 tagged to GST protein (ab43615)
Protocols
References for GST tag ELISA Kit (ab126581)
ab126581 has not yet been referenced specifically in any publications.
