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Western blot - GSTM1 + GSTM2 antibody (ab27489)
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Product Name
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GSTM1 + GSTM2 antibody
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Product type
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Primary antibodies
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Description
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Goat polyclonal to GSTM1 + GSTM2
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Immunogen
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Synthetic peptide: CVFSKMAVWGNK from the C Terminus of the protein (Human).
BLAST 'CVFSKMAVWGNK' with  or 
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Reacts with
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Hu
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Predicted to react
(species key)
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Chmp, CynMk
(Due to sequence homology)
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Specificity
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This antibody is expected to recognise both reported variants of human GSTM1 protein (NP_000552; NP_666533) and GSTM2 protein (NP_000848).
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Tested applications
(see key)
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ELISA, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ELISA: 1/1000.
WB: Use at a concentration of 1 - 3 µg/ml. Detects a band of approximately 26 kDa (predicted molecular weight: 26 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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Human liver and lung lysates for Western blot.
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Cellular localization
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Cytoplasmic
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Research areas
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Signal Transduction >> Metabolism >> Drug metabolism
Tags & Cell Markers >> Cell Type Markers >> Tumor Associated
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Relevance
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Glutathione S transferases are encoded by two distinct supergene families and a total of eight distinct classes of the soluble cytoplasmic mammalian glutathione S transferases have been identified: alpha, kappa, mu, omega, pi, sigma, theta and zeta. The mu class of these enzymes, to which GSTM1 and 2 belong, functions in the detoxification of electrophilic compounds by conjugation with glutathione. The genes encoding the mu class of enzymes are known to be highly polymorphic. These genetic variations can change an individual's susceptibility to carcinogens and toxins as well as affect the toxicity and efficacy of certain drugs and null mutations of this gene have been linked with an increase in a number of cancers.
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GSTM1 + GSTM2 antibody (ab27489) |
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Goat
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: 0.02% Sodium Azide.
Constituents: 0.5% BSA, Tris saline. pH 7.3. Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Purification notes
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This antibody purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
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Form
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Liquid
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Concentration
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0.500 mg/ml
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this GSTM1 + GSTM2 antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for GSTM1 + GSTM2 antibody images, references, products related to ab27489 and other tools.
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GSTM1 + GSTM2 antibody images:
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 Western blot - GSTM1 + GSTM2 antibody (ab27489)
GSTM1 + GSTM2 antibody (ab27489) at 0.3 µg/ml + human lung lysate (35µg protein in RIPA buffer).
Predicted band size : 26 kDa
Observed band size : 26 kDa
Primary incubation was 1 hour. Detected by chemiluminescence.
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References for GSTM1 + GSTM2 antibody (ab27489)
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This product has been used in: (two most recent references)
Yang Y et al. Glutathione S-Transferase-{mu}1 Regulates Vascular Smooth Muscle Cell Proliferation, Migration, and Oxidative Stress. Hypertension : (2009). PubMed: 19822795
Koh-Tan HH et al. Renal and vascular glutathione S-transferase mu is not affected by pharmacological intervention to reduce systolic blood pressure. J Hypertens 27:1575-84 (2009). Rat. PubMed: 19531963
If you publish research using ab27489 please let us know so that we can cite the reference on this datasheet.
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Search PubMed (MEDLINE) for references to GSTM1 + GSTM2
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GSTM1 + GSTM2 antibody - more information
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