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Products:Tags & Cell Markers >> Cell Type Markers >> Tumor Associated
Overview
Product name
Anti-GSTM1 + GSTM2 antibody
Description
Goat polyclonal to GSTM1 + GSTM2
Specificity
This antibody is expected to recognise both reported variants of human GSTM1 protein (NP_000552; NP_666533) and GSTM2 protein (NP_000848).
Tested applications
ELISA, WBmore details
Cross reactivity
Reacts with
Human
Predicted to work with
Chimpanzee, Cynomolgus Monkey
Immunogen
Synthetic peptide: CVFSKMAVWGNK from the C Terminus of the protein (Human).
CVFSKMAVWG NK
Positive control
Human liver and lung lysates for Western blot.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 0.5% BSA, 5mg/ml Tris, pH 7.3
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Purification notes
This antibody purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality
Polyclonal
Isotype
IgG
Research areas
Signal Transduction >> Metabolism >> Drug metabolism
Tags & Cell Markers >> Cell Type Markers >> Tumor Associated
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Western blot - GSTM1 + GSTM2 antibody (ab27489)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab27489 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ELISA
ELISA: 1/1000ELISA: 1/1000
WB: Use a concentration of 1 - 3 µg/ml.Detects a band of approximately 26 kDa (predicted molecular weight: 26 kDa).
Target
Relevance
Glutathione S transferases are encoded by two distinct supergene families and a total of eight distinct classes of the soluble cytoplasmic mammalian glutathione S transferases have been identified: alpha, kappa, mu, omega, pi, sigma, theta and zeta. The mu class of these enzymes, to which GSTM1 and 2 belong, functions in the detoxification of electrophilic compounds by conjugation with glutathione. The genes encoding the mu class of enzymes are known to be highly polymorphic. These genetic variations can change an individual's susceptibility to carcinogens and toxins as well as affect the toxicity and efficacy of certain drugs and null mutations of this gene have been linked with an increase in a number of cancers.
Cellular localization
Cytoplasmic
Alternative names
- Glutathione S alkyltransferase M1 antibody
- Glutathione S alkyltransferase M2 antibody
- Glutathione S transferase Mu 1 antibody
see all
Database links
- Entrez Gene: 2944 Human
- Entrez Gene: 2946 Human
- Omim: 138350 Human
- Omim: 138380 Human
- SwissProt: P09488 Human
- SwissProt: P28161 Human
- Unigene: 279837 Human
- Unigene: 301961 Human
Anti-GSTM1 + GSTM2 antibody images:
Western blot - GSTM1 + GSTM2 antibody (ab27489)
Anti-GSTM1 + GSTM2 antibody (ab27489) at 0.3 µg/ml + human lung lysate (35µg protein in RIPA buffer).
Predicted band size : 26 kDa
Observed band size : 26 kDa
Primary incubation was 1 hour. Detected by chemiluminescence.
References for Anti-GSTM1 + GSTM2 antibody (ab27489)
This product has been referenced in:
- Yang Yet al. Glutathione S-Transferase-{mu}1 Regulates Vascular Smooth Muscle Cell Proliferation, Migration, and Oxidative Stress. Hypertension : (2009).Read more (PubMed: 19822795) »
- Koh-Tan HHet al. Renal and vascular glutathione S-transferase mu is not affected by pharmacological intervention to reduce systolic blood pressure. J Hypertens 27:1575-84 (2009). Rat.Read more (PubMed: 19531963) »
See all 2 publications for this product
Publishing research using ab27489? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"