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Products:Tags & Cell Markers >> Cell Type Markers >> Tumor Associated
Overview
Product name
Anti-GSTM1 antibody
See all GSTM1 products (3) ...
Description
Rabbit polyclonal to GSTM1
Specificity
Ab77925 is specific for GSTM1 but there is a possibility of cross-reactivity with other isoforms of GST.
Tested applications
Cross reactivity
Reacts with
Mouse, Rat, Human
Immunogen
Synthetic peptide: C-EKLKPKYLEELPEK, corresponding to amino acids 121-134 of Mouse GST M1.
C-EKLKPKYL EELPEK
Positive control
Hela cell lysate. Liver.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.05% Sodium Azide
Constituents: 30% Glycerol, 0.15M Sodium chloride, 0.1M Tris glycine, pH 7.4
Concentration
Concentration information loading...
Purity
Protein A purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Signal Transduction >> Metabolism >> Drug metabolism
Tags & Cell Markers >> Cell Type Markers >> Tumor Associated
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Western blot - GSTM1 antibody (ab77925)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab77925 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
- 1 Image
WB
Application notes
WB: Use at a concentration of 0.5 - 2.0 µg/ml for 2 hours at RT. Detects a band of approximately 26 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Function
Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles.
Tissue specificity
Liver (at protein level).
Sequence similarities
Belongs to the GST superfamily. Mu family.
Contains 1 GST C-terminal domain.
Contains 1 GST N-terminal domain.
Cellular localization
Cytoplasm.
Target information above from: UniProt accessionP09488
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Glutathione S-transferase Mu 1 antibody
- GST class-mu 1 antibody
- GST HB subunit 4 antibody
see all
Database links
- Entrez Gene: 2944 Human
- Entrez Gene: 14862 Mouse
- Entrez Gene: 24423 Rat
- Omim: 138350 Human
- SwissProt: P09488 Human
- SwissProt: P10649 Mouse
- SwissProt: P04905 Rat
- Unigene: 301961 Human
see all
Anti-GSTM1 antibody images:
Western blot - GSTM1 antibody (ab77925)
Anti-GSTM1 antibody (ab77925) at 0.5 µg/ml + Hela cell lysate
Secondary
Goat anti-Rabbit HRP conjugated at 1/2000 dilution
developed using the ECL technique
Observed band size : 26 kDa (why is the actual band size different from the predicted?)
Additional bands at : 60 kDa. We are unsure as to the identity of these extra bands.
References for Anti-GSTM1 antibody (ab77925)
ab77925 has not yet been referenced specifically in any publications.
Publishing research using ab77925? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"