Anti-GSTM5 antibody (ab154018)
Key features and details
- Rabbit polyclonal to GSTM5
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-GSTM5 antibody -
Description
Rabbit polyclonal to GSTM5 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Recombinant fragment, corresponding to a region within amino acids 1-218 of Human GSTM5 (Uniprot ID: P46439).
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Positive control
- Mouse and Rat testis lysates; HeLa cells; Human SG xenograft tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab154018 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/5000 - 1/20000. Predicted molecular weight: 26 kDa.
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IHC-P |
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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ICC/IF |
1/100 - 1/1000.
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Notes |
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WB
1/5000 - 1/20000. Predicted molecular weight: 26 kDa. |
IHC-P
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ICC/IF
1/100 - 1/1000. |
Target
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Function
Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles. -
Sequence similarities
Belongs to the GST superfamily. Mu family.
Contains 1 GST C-terminal domain.
Contains 1 GST N-terminal domain. -
Cellular localization
Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 2949 Human
- Entrez Gene: 14866 Mouse
- Entrez Gene: 64352 Rat
- Omim: 138385 Human
- SwissProt: P46439 Human
- SwissProt: P48774 Mouse
- SwissProt: Q9Z1B2 Rat
- Unigene: 75652 Human
see all -
Alternative names
- Glutathione S alkyltransferase M5 antibody
- Glutathione S aralkyltransferase M5 antibody
- Glutathione S aryltransferase M5 antibody
see all
Images
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Anti-GSTM5 antibody (ab154018) at 1/10000 dilution + Mouse testis whole cell lysate at 50 µg
Predicted band size: 26 kDa
12% SDS PAGE -
Anti-GSTM5 antibody (ab154018) at 1/10000 dilution + Rat testis whole cell lysate at 50 µg
Predicted band size: 26 kDa
12% SDS PAGE -
Immunohistochemical analysis of paraffin-embedded Human SG xenograft tissue labeling GSTM5 with ab154018 at 1/500 dilution.
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Immunofluorescent analysis of paraformaldehyde-fixed HeLa cells labeling GSTM5 with ab154018 at 1/200 dilution. Lower image is merged with DNA probe.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab154018 has been referenced in 1 publication.
- Alexander J et al. Offspring sex impacts DNA methylation and gene expression in placentae from women with diabetes during pregnancy. PLoS One 13:e0190698 (2018). PubMed: 29470513