Anti-GTPBP9 antibody (ab51077)

Overview

• Product nameAnti-GTPBP9 antibodySee all GTPBP9 primary antibodies ...
• Description
  Rabbit polyclonal to GTPBP9
• Tested applicationsWB, IHC-P, ICC/IF more details
• Species reactivity
  Reacts with: Human
  Predicted to work with: Mouse, Rat, Cow, Dog
• Immunogen

  A region within synthetic peptide: QGLGNAFLSH ISACDGIFHL TRAFEDDDIT HVEGSVDPIR DIEIIHEELQ, corresponding to amino acids 104-153 of Human GTPBP9

  Run BLAST with Run BLAST with
• Positive controlJurkat cell lysate, Human intestine This antibody gave a positive result in IF in the following Formaldehyde/Methanol fixed cell line: HCT116

Properties

• FormLiquid
• Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
• Storage bufferPreservative: None
  Constituents: 2% Sucrose, PBS
• Concentration information loading...
• PurityImmunogen affinity purified
• Clonality Polyclonal
• IsotypeIgG
• Research Areas
  • Signal Transduction
  • Second Messenger
  • Nucleotide Messenger
  • GTP

  • Cell Biology
  • Other Antibodies
  • Other Antibodies

Applications

Target

• FunctionHydrolyzes ATP, and can also hydrolyze GTP with lower efficiency. Has lower affinity for GTP.
• Sequence similaritiesBelongs to the GTP1/OBG family.
  Contains 1 G (guanine nucleotide-binding) domain.

Target information above from: UniProt accession Q9NTK5 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 29789 Human
  • Entrez Gene: 67059 Mouse
  • Entrez Gene: 296488 Rat
  • Omim: 611175 Human
  • SwissProt: Q9NTK5 Human
  • SwissProt: Q9CZ30 Mouse
  • SwissProt: A0JPJ7 Rat
  • Unigene: 157351 Human

  • Unigene: 22661 Mouse
  • Unigene: 5874 Rat

  see all

• Alternative names
  DKFZp313H1942 antibodyDNA damage-regulated overexpressed in cancer 45 protein antibodyDOC45 antibody

  EC 3.6.3 antibodyGBP45 antibodyGTP-binding protein 9 (putative) antibodyGTP-binding protein 9 antibodyGTP-binding protein PTD004 antibodyhomologous yeast-44.2 protein antibodyObg like ATPase 1 antibodyObg-like ATPase 1 antibodyOLA1 antibodyOLA1_HUMAN antibodyPTD004 antibody

  see all

Anti-GTPBP9 antibody images

• 

  Western blot - GTPBP9 antibody (ab51077)
  Lane 1 : Marker
  Lane 2 : Anti-GTPBP9 antibody (ab51077) at 0.25 µg/ml
  
  Lane 1 : As above
  Lane 2 : Jurkat cell lysate at 10 µg/ml with skim milk/ PBS at 5 %
  
  Secondary
  Lane 2 : HRP conjugated anti-Rabbit IgG at 1/50000 dilution
  
  
  Predicted band size : 45 kDa
  Observed band size : 45 kDa
  
• 

  Immunohistochemistry (Paraffin-embedded sections) - GTPBP9 antibody (ab51077)
  ab51077 at 4.0 µg/ml staining epithelial cells of the intestinal villus from human intestine; paraffin embedded; magnification 400x.
• 

  Immunocytochemistry/ Immunofluorescence - Anti-GTPBP9 antibody (ab51077)
  ab51077 stained HCT116 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab51077 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.