Anti-GTPase HRAS antibody [Y13-259] (ab16881)

Dear technical support agent,
I will be happy to test the antibody and write an abreview. I will order the Ab tomorrow morning. Just let me know what I need to do next.
Many thanks,

ANSWER:

Thank you for your reply. I have created the discount code below, which can be redeemed for a free primary antibody after submitting a review of ab96548 and following the instructions below. Please let me know if you have any questions or if there is anything else that I can help you with.
DISCOUNT CODE: *** Expiration date: June 16, 2012
This code will give you 1 free primary antibody before the expiration date. To redeem this offer, please submit an Abreview for ab96548 with mouse samples and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews.
The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.
Any feedback that you can provide will be greatly appreciated, whether positive or negative. We look forward to receiving your Abreview and wish you luck with your research.
The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.

I am looking for an antibody that is specific for murine H-Ras (no cross reactivity with K- or N-Ras) to be used for WB and/or IHC. Do you have anything interesting? I have already tried your ab32417 and I know that ab16881 cross reacts with N and Kras. What about ab64705 and ab96548? are they suitable for this use? could I have a trial size to test them?
Many thanks,

ANSWER:

Thank you for contacting us with your enquiry.
I have run some alignments between the immunogens of ab64705 and ab96548, and ab96548 is more specific for HRas. The immunogen of ab64705 overlaps quite a bit with N and KRas, however ab96548 shows no significant similarity to KRas and very little (47%) to NRas.
We only have the full size vials of all of our products and are not able to send trial sizes. However, as ab96548 has not been tested in murine samples, you are eligible for our testing discount program if you would like to try this antibody in mouse. For this program, you would initially purchase ab96548, test it in mouse, then submit an Abreview with your data. In exchange we will give you a discount code valid for one free primary antibody on a future order.
I hope this information will be useful. Please let me know if you have any questions, or if you would like to participate in our testing discount program, and I will be happy to arrange that for you.

Hi there, is this antibody specific for H-Ras or does it cross react with N- or K-Ras?

ANSWER:

Thank you for contacting Abcam The antibody, ab16881 is reactive with the phosphorylated and non-phosphorylated forms of v-H-Ras and v-K-Ras and recognizes the p21 translational products of the H-, K-, and N-ras human genes. This antibody does not cross-react with rap. Please let me know if there is anything else I can help you with.