Products:Epigenetics and Nuclear Signaling >> DNA / RNA >> RNA Processing >> RNAi
If your product does not perform as described on this datasheet, we will refund or replace your product...
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Please arrange a discount code for ab87541. Thank you. |
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ANSWER: |
DISCOUNT CODE: [XXXXX] Expiration date: [07/03/2012] I am very pleased to hear you would like to accept our offer and test ab[87541] in [ICC/IF]. This code will give you 1 free [PRIMARY ANTIBODY] before the expiration date. To redeem this offer, please submit an Abreview for [ICC/IF] and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews. Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code. Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research. The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount. |
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P {margin-top:2px;margin-bottom:2px;} Dear technical team, Our customer would like to test ICC/IF with human sample. She searched ab70522 but, now it's not available so, Please recommend one of your GW182 antibody to our customer. She would like to get a discount code for it. I look forward to hearing from you. Best regards, |
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ANSWER: |
Thank you for contacting us. We have 3 antibodies available in catalogue ab114857, ab87541 and ab84403. These antibodies are Rabbit polyclonal to GW182. Could you check the datasheets and select the product which is suitable for you. All of these products are eligible for Abreview discount programme. Let me know the product you would be interested in using I will then send you the discount code. I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information. |
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
ab70522 (1/200) staining GW182 in assynchronous HeLa cells (green). Cells were fixed in paraformaldehyde, permeabilised with Triton X100/PBS and counterstained with DAPI in order to highlight the nucleus (red). Please refer to abreview for further experimental details.
This image is courtesy of an Abreview submitted by Dr Kirk McManus
IF image of ab70522 stained MCF7 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab70522, 1µg/ml) overnight at +4ºC. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM
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