Anti-GW182 antibody [4B6] - P/GW Body Marker (ab70522)

  Immunocytochemistry/ Immunofluorescence - GW182 antibody [4B6] - P/GW Body Marker (ab70522)

ab70522 (1/200) staining GW182 in assynchronous HeLa cells (green). Cells were fixed in paraformaldehyde, permeabilised with Triton X100/PBS and counterstained with DAPI in order to highlight the nucleus (red). Please refer to abreview for further experimental details.

This image is courtesy of an Abreview submitted by Dr Kirk McManus

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  Immunocytochemistry/ Immunofluorescence - GW182 antibody [4B6] - P/GW Body Marker (ab70522)

IF image of ab70522 stained MCF7 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab70522, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM