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Products:Epigenetics and Nuclear Signaling >> DNA / RNA >> RNA Processing >> RNAi
Overview
Product name
Anti-GW182 antibody
See all GW182 products (4) ...
Description
Rabbit polyclonal to GW182
Specificity
ab84403 recognises isoform 2 of GW182.
Tested applications
WB, ELISA, IHC-Pmore details
Cross reactivity
Reacts with
Mouse, Rat, Human
Immunogen
A 16 amino acid peptide from near the C terminal residues of human GW182 protein (NM_020847; Q8NDV7).
Positive control
Brain lysates.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 1% BSA
Concentration
Concentration information loading...
Purity
Protein A purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Epigenetics and Nuclear Signaling >> RNAi >> P bodies/GW bodies
Epigenetics and Nuclear Signaling >> DNA / RNA >> RNA Processing >> RNAi
Applications
Show applications key
Our Abpromise guarantee covers the use of ab84403 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/200 - 1/500.Predicted molecular weight: 182 kDa.
ELISA: Use at an assay dependent dilution.
IHC-P: Use a concentration of 1 µg/ml
Target
Function
Plays a role in RNA-mediated gene silencing by both micro-RNAs (miRNAs) and short interfering RNAs (siRNAs). Required for miRNA-dependent repression of translation and for siRNA-dependent endonucleolytic cleavage of complementary mRNAs by argonaute family proteins.
Tissue specificity
Ubiquitous.
Sequence similarities
Belongs to the GW182 family.
Contains 1 RRM (RNA recognition motif) domain.
Cellular localization
Cytoplasm > P-body. Mammalian P-bodies are also known as GW bodies.
Target information above from: UniProt accessionQ8NDV7
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- CAG repeat protein 26 antibody
- CAGH26 antibody
- DKFZp666E117 antibody
see all
Database links
- Entrez Gene: 27327 Human
- Entrez Gene: 233833 Mouse
- Entrez Gene: 308971 Rat
- Omim: 610739 Human
- SwissProt: Q8NDV7 Human
- SwissProt: Q3UHK8 Mouse
- Unigene: 407740 Human
- Unigene: 655057 Human
see all
Anti-GW182 antibody images:
Western blot - GW182 antibody (ab84403)
Anti-GW182 antibody (ab84403) at 1/200 dilution + human fetal brain lysates
Predicted band size : 182 kDa
Observed band size : 182 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GW182 antibody (ab84403)
IHC image of ab84403 staining in Breast Cancer formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab84403, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
References for Anti-GW182 antibody (ab84403)
This product has been referenced in:
- Chen Set al. Ago-2-Mediated Slicer Activity Is Essential for Anti-Flaviviral Efficacy of RNAi. PLoS One 6:e27551 (2011). WB; Human.Read more (PubMed: 22102908) »
See 1 publication for this product
Publishing research using ab84403? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"