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Western blot - Gag polyprotein antibody (ab36551)
(enlarge)
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Product Name
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Gag polyprotein antibody
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Product type
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Primary antibodies
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Description
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Chicken polyclonal to Gag polyprotein
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Immunogen
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Synthetic peptide, corresponding to N terminal amino acids 2-132 of HIV Gag polyprotein.
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Reacts with
(species key)
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N/A
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Tested applications
(see key)
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WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
WB: 1/2000. Detects a band of approximately 76 kDa (predicted molecular weight: 56 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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E. coli derived HIV matrix fusion protein.
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Cellular localization
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Cytoplasmic and Nuclear
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Research areas
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Microbiology >> Organism >> Virus >> RNA Virus >> ssRNA positive strand virus >> HIV
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Relevance
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HIV Gag polyprotein contains: matrix protein p17 (MA), capsid protein p24 (CA), spacer peptide p2, nucleocapsid protein p7 (NC), spacer peptide p1 and p6. Specific enzymatic cleavages by the viral protease yield mature proteins. The polyprotein is cleaved during and after budding; this process is termed maturation.
Matrix protein p17 targets Gag and Gag-Pol polyproteins to the plasma membrane via a multipartite membrane binding signal, that includes its myristoylated N terminus. It also mediates nuclear localization of the preintegration complex and is implicated in release of HIV from the host cell mediated by Vpu. Capsid protein p24 forms the conical core of the virus that encapsulates the genomic RNA nucleocapsid complex. Nucleocapsid protein p7 encapsulates and protects viral dimeric unspliced (genomic) RNA. It binds these RNAs through its zinc fingers. p6 is thought to play a role in budding of the assembled particle by interacting with the host class E VPS proteins TSG101 and PDCD6IP/AIP1. Matrix protein p17 is a trimer. It interacts with gp120. p6 also interacts with human TSG101 and PDCD6IP/AIP1.
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Raised in
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Chicken
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Clonality
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Polyclonal
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Isotype
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IgY
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: None
Constituents: PBS
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Form
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Liquid
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Storage instructions
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Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this Gag polyprotein antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for Gag polyprotein antibody images, references, products related to ab36551 and other tools.
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Gag polyprotein antibody images:
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 Western blot - Gag polyprotein antibody (ab36551)
Gag polyprotein antibody (ab36551) at 1/2000 dilution + E. coli derived HIV matrix fusion protein
Secondary
Goat anti IgY HRP at 1/1000 dilution
Predicted band size : 56 kDa
Observed band size : 76 kDa (why is the actual band size different from the predicted?)
The arrow next to the image points to the HIV matrix fusion protein.
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Search PubMed (MEDLINE) for references to Gag polyprotein
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Gag polyprotein antibody - more information
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Customer reviews (feedback) regarding Gag polyprotein antibody |
Customer FAQs regarding Gag polyprotein antibody |
Protocols for Gag polyprotein antibody |
Price and availability of products related to Gag polyprotein antibody |
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Gag polyprotein antibody - related products:
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Gag polyprotein antibody - other tools:
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Contact Abcam with a Technical Enquiry about Gag polyprotein antibody |
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