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Galactose Assay Kit (ab83382)

Q&A (4)

Overview

  • Product nameGalactose Assay Kit
  • Tests
    100 x 1 assay
  • Sample type
    Cell culture supernatant, Urine, Serum, Plasma, Other biological fluids, Tissue Extracts
  • Assay typeQuantitative
  • Sensitivity
    < 10 nmol/well
  • Assay time
    0h 40m
  • Product overview

    Abcam's Galactose Assay Kit provides a simple, convenient means for direct measurement of galactose levels in various biological samples (serum, plasma, other body fluids, food, growth media, etc.). In the assay kit, Galactose is specifically oxidized generating a product that produces color (OD570nm) and fluorescence (Ex/Em 535/587 nm). Liquid samples can be tested directly without purification. The assay is fast, convenient and sensitive. It can also be used as a high throughput assay.
    Visit our FAQs page for tips and troubleshooting.

  • Notes

    Galactose (C6H12O6 FW: 180.16) is one the major naturally occurring sugars, involved in many biological processes.

  • Tested applicationsFunctional Studies more details

Properties

  • RelevanceGalactose (C6H12O6 FW: 180.16) is one the major naturally occurring sugars, involved in many biological processes.

    • Applications

    Our Abpromise guarantee covers the use of ab83382 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    Functional Studies FuncS

    Galactose Assay Kit images

    • Western blot - Galactose Assay Kit (ab83382)
      Western blot - Galactose Assay Kit (ab83382)
      Example of Galactose standard curve obtained with ab83382

    Protocols

    References for Galactose Assay Kit (ab83382)

    ab83382 has not yet been referenced specifically in any publications.

    Publishing research using ab83382 ? Please let us know so that we can cite the reference in this datasheet

    Product Wall

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    Displaying 1 - 4 of 4 results for Abreviews and Q&A

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    Thanks a lot for your reply.

    Did you get some information in regards to minimal possible rate of the detection of galactosidase (more specific than only < 10 nmol/well that the datasheet indicates).

    I think that I wasn't clear: th...

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    Thank you for your message
    I can confirm that this kit (ab83382: Galactose Assay Kit) will measure only galactose. The detection range which your customer has is not associated with this kit. All we mention on the product datasheet is that this kit i...

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    Dear Tech Support Team,

    Our customer wonders if ab83382 detects free galactose or galactose bound to the protein can be also measured. This customer measures glycosylation rate of the proteins in condition medium.

    Thank you for the a...

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    Thank you for your enquiry.
    The ab83382 assay is for galactose. There is not going to be any measurement of enzyme activity in this kit. This kit will measure the total amount of free galactose in your samples.
    If you need any further assistance...

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    I would like to get the galactose standard in this kit separately. Do you sell it?

    Thank you for your patience with this request.

    The datasheet team added this standard as ab140312. It should be available on the catalogue in a few days.

    I hope this information helps. Please contact us with any other questions.

    Phone call requesting information on exactly what the 100 tests provided with this kit encompass. Does it include the standard curve?

    Thank you for contacting us.


    With one Galactose Assay Kit (ab83382) you are provided with enough reagents for a total of 100 tests. As discussed over the phone this does include all the control assays that need to be performed (so taking t...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"