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Galactose and Lactose Assay Kit (ab83378)

Q&A (1)

Overview

  • Product nameGalactose and Lactose Assay Kit
  • Tests
    100 x 1 assay
  • Sample type
    Cell culture supernatant, Urine, Serum, Plasma, Other biological fluids, Tissue Extracts
  • Assay typeQuantitative
  • Assay time
    1h 00m
  • Product overview

    Abcam's Galactose and Lactose Assay Kit provides a simple, convenient means for direct measurement of galactose and lactose levels in various biological samples (serum, plasma, other body fluids, food, growth media, etc.). To detect Galactose, galactose oxidase specifically oxidizes free galactose, generating a product that reacts with the Galactose Probe to produce color (OD570nm) and fluorescence (Ex/Em 535/587). To detect lactose, lactose is hydrolyzed by lactase to generate free galactose which is then measured. Thus, Lactose level = Total Galactose – Free Galactose.
    Visit our FAQs page for tips and troubleshooting.

  • Notes

    Galactose (C6H12O6 FW: 180.16) and lactose (C12H22O11 FW: 342.3) are naturally occurring sugars. Lactose consists of one galactose and one glucose. Some people, particularly infants, lack the enzyme necessary to digest galactose leading to galactose accumulation in blood (Galactosemia) causing enlarged liver, renal failure, cataracts and brain damage.

  • Tested applicationsFunctional Studies more details

Properties

  • Storage instructionsStore at -20°C. Please refer to protocols.
  • Components Identifier 100 tests
    Galactose Assay Buffer WM 1 x 25ml
    Galactose Enzyme Mix (Lyophilized) Green 1 vial
    Galactose Probe (DMSO solution) Red 1 x 200µl
    Galactose Standard (100nmol/ul) Yellow 1 x 100µl
    HRP (Lyophilized) Purple 1 vial
    Lactase (Lyophilized) Blue 1 vial
  • Research Areas
  • RelevanceGalactose (C6H12O6 FW: 180.16) and lactose (C12H22O11 FW: 342.3) are naturally occurring sugars. Lactose consists of one galactose and one glucose. Some people, particularly infants, lack the enzyme necessary to digest galactose leading to galactose accumulation in blood (Galactosemia) causing enlarged liver, renal failure, cataracts and brain damage.

    • Applications

    Our Abpromise guarantee covers the use of ab83378 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    Functional Studies FuncS

    Galactose and Lactose Assay Kit images

    • Functional Studies - Galactose and Lactose Assay Kit (ab83378)
      Functional Studies - Galactose and Lactose Assay Kit (ab83378)
      Example of a Colorimetric standard curve using ab83378.

    Protocols

    References for Galactose and Lactose Assay Kit (ab83378)

    ab83378 has not yet been referenced specifically in any publications.

    Publishing research using ab83378 ? Please let us know so that we can cite the reference in this datasheet

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    Customer needs to measureLactose (mainly) in human breast milk.
    Which one of the kits (ab83378or ab83384)is more suitable for him?
    Protocol of ab83378 states that a maximum of 0.1 ul milk can be used. Is this small amount correct? If so, could the...

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    Bitte entschuldigen Sie Verspätung meiner Antwort.
    Ich habe endlich Nachricht aus dem Labor erhalten:
    "If the client is interested only in Lactose measurement, I would highly recommend him to go with the ab83384 kit. If his emphasis is on lactose,...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"