Galactose and Lactose Assay Kit (ab83378)
- Product nameGalactose and Lactose Assay Kit
- Tests100 x 1 assay
- Sample typeCell culture supernatant, Urine, Serum, Plasma, Other biological fluids, Tissue Extracts
- Assay typeQuantitative
- Assay time1h 00m
- Product overview
Abcam's Galactose and Lactose Assay Kit provides a simple, convenient means for direct measurement of galactose and lactose levels in various biological samples (serum, plasma, other body fluids, food, growth media, etc.). To detect Galactose, galactose oxidase specifically oxidizes free galactose, generating a product that reacts with the Galactose Probe to produce color (OD570nm) and fluorescence (Ex/Em 535/587). To detect lactose, lactose is hydrolyzed by lactase to generate free galactose which is then measured. Thus, Lactose level = Total Galactose – Free Galactose.
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Galactose (C6H12O6 FW: 180.16) and lactose (C12H22O11 FW: 342.3) are naturally occurring sugars. Lactose consists of one galactose and one glucose. Some people, particularly infants, lack the enzyme necessary to digest galactose leading to galactose accumulation in blood (Galactosemia) causing enlarged liver, renal failure, cataracts and brain damage.
- Tested applicationsFunctional Studies more details
- Storage instructionsStore at -20°C. Please refer to protocols.
Components Identifier 100 tests Galactose Assay Buffer WM 1 x 25ml Galactose Enzyme Mix (Lyophilized) Green 1 vial Galactose Probe (DMSO solution) Red 1 x 200µl Galactose Standard (100nmol/ul) Yellow 1 x 100µl HRP (Lyophilized) Purple 1 vial Lactase (Lyophilized) Blue 1 vial
- Research Areas
Our Abpromise guarantee covers the use of ab83378 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
References for Galactose and Lactose Assay Kit (ab83378)
ab83378 has not yet been referenced specifically in any publications.