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Overview

  • Product nameGalectin 1 Mouse ELISA KitSee all Galectin 1 kits ...
  • Tests
    1 x 96 well plate
  • Sample type
    Cell culture supernatant, Serum, Plasma, Other biological fluids, Tissue Extracts
  • Assay typeSandwich
  • Sensitivity
    < 5 pg/ml
  • Range
    156 pg/ml - 10000 pg/ml
  • Species reactivity
    Reacts with: Mouse
  • Product overview

    ab119595 Galectin 1 Mouse ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. Mouse GALECTIN-1 specific-specific polyclonal antibodies were precoated onto 96-well plates. The mouse specific detection polyclonal antibodies were biotinylated. The test samples and biotinylated detection antibodies were added to the wells subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse GALECTIN-1 amount of sample captured in plate.

     

    No detectable cross-reactivity with any other cytokine.

  • Notes

    Store at 4°C for frequent use, at -20°C for infrequent use. Avoid multiple freeze-thaw cycles.

  • Tested applicationsSandwich ELISA more details

Properties

  • FunctionMay regulate apoptosis, cell proliferation and cell differentiation. Binds beta-galactoside and a wide array of complex carbohydrates. Inhibits CD45 protein phosphatase activity and therefore the dephosphorylation of Lyn kinase.
  • Tissue specificityExpressed in placenta, maternal decidua and fetal membranes. Within placenta, expressed in trophoblasts, stromal cells, villous endothelium, syncytiotrophoblast apical membrane and villous stroma. Within fetal membranes, expressed in amnion, chorioamniotic mesenchyma and chorion (at protein level). Expressed in cardiac, smooth, and skeletal muscle, neurons, thymus, kidney and hematopoietic cells.
  • Sequence similaritiesContains 1 galectin domain.
  • Cellular localizationSecreted > extracellular space > extracellular matrix.

    • Target information above from: UniProt accession P09382 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
    • Alternative names
        14 kDa laminin-binding protein14 kDa lectinBeta galactoside binding protein 14kDa
        Beta-galactoside-binding lectin L-14-IGal-1GAL1GalaptinGalectin-1GBPHBLHLBP14HPLLactose-binding lectin 1Lectin galactoside-binding soluble 1LEG1_HUMANLGALS1Putative MAPK-activating protein PM12S-Lac lectin 1
      see all
  • Database links

      • Applications

      Our Abpromise guarantee covers the use of ab119595 in the following tested applications.

      The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

      Application Notes
      Sandwich ELISA sELISA

      Galectin 1 Mouse ELISA Kit images

      • Sandwich ELISA - Galectin 1 Mouse ELISA Kit (ab119595)
        Sandwich ELISA - Galectin 1 Mouse ELISA Kit (ab119595)
        Representative standard curve using ab119595.

      Protocols

      References for Galectin 1 Mouse ELISA Kit (ab119595)

      ab119595 has not yet been referenced specifically in any publications.

      Publishing research using ab119595 ? Please let us know so that we can cite the reference in this datasheet

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      Thank you very much for your prompt and helpful reply. After discussing the situation with my professor, we have decided that a refund would be best.

      The only signal we saw was from a cell culture freeze thaw extract that was had a very high co...

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      I am sorry that thiskit did not perform as stated on the datasheet. If payment has already been made on the original order and you wish to receive a refund, please ask your purchasi...

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      Inquiry: To Whom It May Concern, we ordered an abcam Galectin 1 Mouse ELISA Kit, ab119595. It was stored at 4°C immediately and continuously. Having used this kit previously (different lot) reasonably successfully, I was expecting little problem conti...

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      I am sorry that this kit did not work as it should have. I'm happy to offer you a free of charge replacement, credit, or refund. Which would you prefer?

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      Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"