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Anti-Galectin 1 antibody (ab38328)

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Overview

Product name

Anti-Galectin 1 antibody
See all Galectin 1 products (10) ...

Description

Rabbit polyclonal to Galectin 1

Tested applications

WBmore details

Cross reactivity

Reacts with

Human, Chinese Hamster

Predicted to work with

Mouse, Rat, Sheep, Cow, Pig, Orangutan

Immunogen

Synthetic peptide conjugated to KLH derived from within residues 50 to the C-terminus of Human Galectin 1.

Read Abcam's proprietary immunogen policy

(Peptide available as ab383 27.)

Positive control

ab38328 gave a positive signal in the following Human Lysates: Heart, Skeletal Muscle, Kidney (Data not shown), Thymus (Data not shown), HeLa, A431, HL60 (Data not shown), Ramos (Data not shown) and THP1 (Data not shown).

Properties

Form

Liquid

Storage instructions

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Storage buffer

Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4

Concentration

Concentration information loading...

Purity

Immunogen affinity purified

Clonality

Polyclonal

Isotype

IgG

Research areas

Cell Biology >> Cell Cycle >> Cell differentiation
Cell Biology >> Apoptosis >> Receptors >> Associated Proteins

  • Western blot - Galectin 1 antibody (ab38328)Western blot - Galectin 1 antibody (ab38328) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab38328 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • ShowHide1 Image

    WB

     WB: Use a concentration ...Read more →

    WB: Use a concentration of 1 µg/ml. Detects a band of approximately 14 kDa (predicted molecular weight: 14 kDa).

Target

Function

May regulate apoptosis, cell proliferation and cell differentiation. Binds beta-galactoside and a wide array of complex carbohydrates. Inhibits CD45 protein phosphatase activity and therefore the dephosphorylation of Lyn kinase.

Tissue specificity

Expressed in placenta, maternal decidua and fetal membranes. Within placenta, expressed in trophoblasts, stromal cells, villous endothelium, syncytiotrophoblast apical membrane and villous stroma. Within fetal membranes, expressed in amnion, chorioamniotic mesenchyma and chorion (at protein level). Expressed in cardiac, smooth, and skeletal muscle, neurons, thymus, kidney and hematopoietic cells.

Sequence similarities

Contains 1 galectin domain.

Cellular localization

Secreted > extracellular space > extracellular matrix.

Target information above from: UniProt accessionP09382 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • 14 kDa laminin-binding protein antibody
  • 14 kDa lectin antibody
  • Beta galactoside binding lectin antibody
  • Beta galactoside binding lectin L 14 I antibody
  • beta galactoside binding protein antibody
  • Beta-galactoside-binding lectin L-14-I antibody
  • DKFZp686E23103 antibody
  • Gal 1 antibody
  • Gal-1 antibody
  • Galaptin antibody
  • Galbp antibody
  • Galectin antibody
  • Galectin-1 antibody
  • Galectin1 antibody
  • GBP antibody
  • HBL antibody
  • HLBP14 antibody
  • HPL antibody
  • L 14.5 antibody
  • L-14.5 antibody
  • L14 antibody
  • Lactose binding lectin 1 antibody
  • Lactose-binding lectin 1 antibody
  • Lect14 antibody
  • Lectin galactoside binding soluble 1 antibody
  • Lectin galactoside-binding soluble 1 antibody
  • LEG1_HUMAN antibody
  • LGALS 1 antibody
  • LGALS1 antibody
  • Lgals1 lectin galactose binding soluble 1 antibody
  • MAPK activating protein MP12 antibody
  • Putative MAPK activating protein MP12 antibody
  • Putative MAPK-activating protein PM12 antibody
  • S Lac lectin 1 antibody
  • S-Lac lectin 1 antibody
see all

Database links

see all

Anti-Galectin 1 antibody images:

  Western blot - Galectin 1 antibody (ab38328)

Western blot - Galectin 1 antibody (ab38328)

All lanes : Anti-Galectin 1 antibody (ab38328) at 1 µg/ml

Lane 1 : Heart (Human) Tissue Lysate - adult normal tissue (ab29431)
Lane 2 : Skeletal Muscle (Human) Tissue Lysate - adult normal tissue (ab29330)
Lane 3 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 4 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate

Lysates/proteins at 10 µg per lane.

Secondary
IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution

Performed under reducing conditions.

Predicted band size : 14 kDa
Observed band size : 14 kDa
Additional bands at : 34 kDa,50 kDa,70 kDa. We are unsure as to the identity of these extra bands.

References for Anti-Galectin 1 antibody (ab38328)

This product has been referenced in:

  • Woolley JF & Al-Rubeai M The isolation and identification of a secreted biomarker associated with cell stress in serum-free CHO cell culture. Biotechnol Bioeng 104:590-600 (2009). WB; Chinese Hamster.Read more (PubMed: 19544303) »

See 1 publication for this product

Publishing research using ab38328? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"