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Galectin 3 protein (His tag) (ab89487)

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Overview

  • Product nameGalectin 3 protein (His tag)See all Galectin 3 proteins and peptides ...
  • Protein descriptionRecombinant full length Human Galectin 3 with His tag; amino acids 1-250 (270aa), 28.3kDa. Swiss ID = P17931.
  • Expression hostE. coli

    • Properties

  • Purity> 95 % by SDS-PAGE
  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: 10% Glycerol, 20mM Tris HCl, 1mM DTT, pH 8.0
  • Concentration information loading...
  • Sequence notesab89487 is purified using conventional chromatography techniques.
  • Research Areas

    • Applications

    Our Abpromise guarantee covers the use of ab89487 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    SDS-PAGE SDS-PAGE: Use at an assay dependent dilution.

    Protein info

    • Alternative names
        35 kDa lectinCarbohydrate binding protein 35Carbohydrate-binding protein 35
        CBP 35CBP35Gal-3GAL3Galactose-specific lectin 3Galactoside-binding proteinGALBPGalectin 3 internal gene,includedGalectin-3Galectin3GALIGGBPIgE binding proteinIgE-binding proteinL 31L 34L-31L-34 galactoside-binding lectinL31Laminin-binding proteinLectin L-29Lectin, galactose binding, soluble 3LEG3_HUMANLGALS2LGALS3MAC 2 antigenMac-2Mac-2 antigenMAC2Macrophage galactose-specific lectinMGC105387
      see all
  • FunctionGalactose-specific lectin which binds IgE. May mediate with the alpha-3, beta-1 integrin the stimulation by CSPG4 of endothelial cells migration. Together with DMBT1, required for terminal differentiation of columnar epithelial cells during early embryogenesis.
  • Tissue specificityA major expression is found in the colonic epithelium. It is also abundant in the activated macrophages.
  • Sequence similaritiesContains 1 galectin domain.
  • Cellular localizationNucleus. Cytoplasmic in adenomas and carcinomas. May be secreted by a non-classical secretory pathway and associate with the cell surface.

    • Target information above from: UniProt accession P17931 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt

    Galectin 3 protein (His tag) images

    • SDS-PAGE - Galectin 3 protein (His tag) (ab89487)
      SDS-PAGE - Galectin 3 protein (His tag) (ab89487)
      15% SDS-PAGE showing ab89487 at approximately 28.3kDa (3µg).

    References for Galectin 3 protein (His tag) (ab89487)

    ab89487 has not yet been referenced specifically in any publications.

    Publishing research using ab89487 ? Please let us know so that we can cite the reference in this datasheet

    Product Wall

    Submit an Abreview for ab89487 Submit a Question for ab89487

    Displaying 1 - 9 of 9 results for Abreviews and Q&A

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    How did you measure the concentration of this galectin-3?

    Thank you for contacting us. The concentration for ab89487 was determined by Bradford assay. I hope this helps, please let me know if you need any additional information or assistance.

     Thank you. Do you know when approximately we can expect the delivery of the replacement vial? Regards,  

    Thank you for your response. We are expecting the new stock any time in our US office and hopefully we can dispatch the item this week sometimes. Thank you for your understanding and patien

    Thank you. Looking forward to hearing from you. Regards,

    Thank you for your patience. This is to let you know that I have placed a new order for you - for one vial of ab89487 as a free of charge replacement and the new order number is 978590. Currently, we are waiting for the new stock and as soon as we get...

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    I don’t understand your statements about the lots from your email. On your shipment vials you specify that is different lot or batch or whatever you call it. All I know is that in my assays only  2 free of charge replacement vials (GR 46331-3 ) worked. An...

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    Thank you for getting back to me. I completely understand your frustration and try to do my best to solve this problem as soon as possible. I would like to update you on this issue: The lot number is an identification number for the synthe...

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    Thank you. Looking forward to hearing from you. Regards,

    Thank you for your response. We can provide new vial from the same lot. After reviewing and discussing this issue carefully with the originator, I would like to clarify that both Abcam  batch numbers GR 46331-3 (two free of charge replacement vials No:959...

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    The replacement vials that you have sent, lot number #GR 46331-3, are working very well in my assay. As I have stated in my email I have purchased the new vial just last week, so that means after I have received the replacement vials. I wanted to make sur...

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    Thank you for your enquiry and for getting back to me. I am very pleased to hear that that the two replacement vials from the very new batch worked fine. Just to clarify the new vials were specifically prepared for you and the new batch is still n...

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    Thank you very much for the replacement vials of His-tagged galectin-3 that was delivered at the beginning of October. However I have to inform you that I have just bought a new vial of 100 µg of galectin-3 (PO#972126) that is from the old batch (lot #GR ...

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    Thank you for your recent message. Could you please confirm if the replacement vials which were arranged on Order NO: 959627 worked as expected or not? This information would be very important for us and for our Lab. Did you purchase the new vial (GR ...

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    Thank you very much for your email. I will appreciate very much if you could ship the replacement vials at your earliest convenience.Best regards,

    Thank you for getting back to me. We will very shortly send you 2 vials from our new batch as free of charge replacements. We sincerely apologize for any inconvenience caused and thank you for bringing this matter to our attention. Pl...

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    please find my answers to your questions below: The two batches (GR46331-1 and GR46331-2) are from the same purification. This means that there should be no difference between these two vials. If these two samples are from the same purifica...

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    Thank you for providing some further details. I have contacted you on the 27th September but still not heard from you. To see whether it was an isolated case, we re-verified our previous tests and tested other vials from the same lot. We believe i...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"