Human Galectin 7 ELISA Kit (GAL7) (ab100523)
Key features and details
- Sensitivity: 20 pg/ml
- Range: 13.72 pg/ml - 10000 pg/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human Galectin 7 ELISA Kit (GAL7)
See all Galectin 7 kits -
Detection method
Colorimetric -
Sample type
Cell culture supernatant, Serum, Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
< 20 pg/ml -
Range
13.72 pg/ml - 10000 pg/ml -
Recovery
> 90 %
Sample specific recovery Sample type Average % Range Cell culture supernatant 96.55 87% - 105% Serum 107.1 95% - 115% Plasma 91.82 70% - 125% -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Human -
Product overview
Abcam’s Galectin 7 (GAL7) Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human Galectin 7 in serum, plasma, and cell culture supernatants.
This assay employs an antibody specific for Human Galectin 7 coated on a 96-well plate. Standards and samples are pipetted into the wells and Galectin 7 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human Galectin 7 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Galectin 7 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
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Notes
Optimization may be required with urine samples
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Platform
Microplate
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 96 tests 200X HRP-Streptavidin Concentrate 1 x 200µl 20X Wash Buffer 1 x 25ml 5X Assay Diluent B 1 x 15ml Assay Diluent A 1 x 30ml Biotinylated anti-Human Galectin 7 2 vials Galectin 7 Microplate (12 x 8 well strips) 1 unit Galectin 7 Standard Human (recombinant) (lyophilized) 2 vials Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml -
Research areas
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Relevance
Galectins belong to a family of related beta-galactoside-binding lectins, also referred to as S-type or S-Lac lectins. Members of this family have been implicated in a variety of functions, including growth regulation, cell adhesion, migration, neoplastic transformation, and immune responses. Galectin 7 is thought to be involved in cell-cell and/or cell-matrix interactions necessary for normal growth control. Galectin 7 has been implicated in apoptosis and is believed to upregulate MMP9 and as such be involved in the progression of tumorigenesis. -
Alternative names
- GAL7
- HKL14
- Human keratinocyte lectin 14
see all -
Database links
- SwissProt: P47929 Human
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab100523 has not yet been referenced specifically in any publications.