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Anti-Gastric Mucin antibody [CLH5] (ab49462)

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Overview

Product name

Anti-Gastric Mucin antibody [CLH5]
See all Gastric Mucin products (2) ...

Description

Mouse monoclonal [CLH5] to Gastric Mucin

Tested applications

IHC-Pmore details

Cross reactivity

Reacts with

Human

Immunogen

Synthetic peptide of the Gastric Mucin (Muc-6) tandem repeat sequence purified by HPLC.

Positive control

Human stomach

Properties

Form

Liquid

Storage instructions

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Storage buffer

Preservative: 15mM Sodium Azide
Constituents: Tissue culture supernatant

Purity

Tissue culture supernatant

Clonality

Monoclonal

Clone number

CLH5

Isotype

IgG1

Research areas

Cancer >> Invasion/microenvironment >> ECM >> Extracellular matrix >> MUC
Signal Transduction >> Cytoskeleton / ECM >> Extracellular Matrix >> ECM Proteins >> MUC

  • Immunohistochemistry (Paraffin-embedded sections) - Gastric Mucin antibody [CLH5] (ab49462)Immunohistochemistry (Paraffin-embedded sections) - Gastric Mucin antibody [CLH5] (ab49462) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab49462 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application notes

IHC-P: 1/50 - 1/100.
High temperature antigen retrieval using 0.01M citrate retrieval solution (pH 6.0) is recommended.

Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Target

Function

May provide a mechanism for modulation of the composition of the protective mucus layer related to acid secretion or the presence of bacteria and noxious agents in the lumen. Play an important role in the cytoprotection of epithelial surfaces and are used as tumor markers in a variety of cancers. May play a role in epithelial organogenesis.

Tissue specificity

Expressed in the regenerative zone of gastric antrum, gastric body mucosa and gastric incisura mucosa. Expressed in the deeper mucous glands of gastric antrum. Overexpressed in Helicobacter pylori infected gastric epithelium. Highly expressed in duodenal Brunner's glands, gall bladder, seminal vesicle, pancreatic centroacinar cells and ducts, and periductal glands of the common bile duct.

Sequence similarities

Contains 1 CTCK (C-terminal cystine knot-like) domain.
Contains 1 TIL (trypsin inhibitory-like) domain.
Contains 3 VWFD domains.

Developmental stage

Early expressed in fetal development and was observed in Brunner's glands and pancreatic ducts at 18-19 weeks and in gastric glands at 20 weeks of gestation. Expressed transiently in the nephrogenic zone of the kidney in the early mid-trimester of development. Detected in the epithelium of ureteric buds at 13 weeks and at lower levels from 17 to 23 weeks of gestation.

Post-translational
modifications

O-glycosylated.

Cellular localization

Secreted.

Target information above from: UniProt accessionQ6W4X9 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • Gastric mucin 6 antibody
  • Gastric mucin-6 antibody
  • MUC 6 antibody
  • MUC-6 antibody
  • Muc6 antibody
  • MUC6 Fragment antibody
  • MUC6 mucin antibody
  • MUC6 mucin Fragment antibody
  • MUC6_HUMAN antibody
  • Mucin 6 antibody
  • Mucin 6 gastric antibody
  • Mucin 6 oligomeric mucus/gel forming antibody
  • Mucin glycoprotein Fragment antibody
  • Mucin-6 antibody
  • Secretory mucin MUC6 Fragment antibody
see all

Database links

Anti-Gastric Mucin antibody [CLH5] images:

  Immunohistochemistry (Paraffin-embedded sections) - Gastric Mucin antibody [CLH5] (ab49462)

Immunohistochemistry (Paraffin-embedded sections) - Gastric Mucin antibody [CLH5] (ab49462)

Ab49462, at a dilution of 1/50, staining Gastric Mucin in paraffin section of normal human stomach by Immunohistochemistry. Note cytoplasmic staining of mucus secreting cells of the deep glands.

References for Anti-Gastric Mucin antibody [CLH5] (ab49462)

This product has been referenced in:

  • Chan MCet al. In Vitro Whole-Virus Binding of a Norovirus Genogroup II Genotype 4 Strain to Cells of the Lamina Propria and Brunner's Glands in the Human Duodenum. J Virol 85:8427-30 (2011). IHC-P.Read more (PubMed: 21680503) »
  • Lennerz JKet al. The Transcription Factor MIST1 Is a Novel Human Gastric Chief Cell Marker Whose Expression Is Lost in Metaplasia, Dysplasia, and Carcinoma. Am J Pathol 177:1514-33 (2010). IHC-P; Human.Read more (PubMed: 20709804) »

See all 3 publications for this product

Publishing research using ab49462? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"