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Synthetic peptide conjugated to KLH derived from within residues 500 to the C-terminus of Human Gata6.
(Peptide available as ab24410.)
Our Abpromise guarantee covers the use of ab22600 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC||Use at an assay dependent concentration. ab171870-Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.|
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 45 kDa (predicted molecular weight: 45 kDa).Can be blocked with Human Gata6 peptide (ab24410) or Human Gata6 peptide (ab24411). Abcam recommends using milk as the blocking agent.|
Image courtesy of Human Protein Atlas
ab22600 staining Gata6 in Human stomach, with strong staining within the glandular cells. Paraffin embedded human stomach was incubated with ab22600 (1/80 dilution) for 30 mins at room temperature. Antigen retrieval was performed by heat induction in citrate buffer pH 6.
ab22600 was tested in a tissue microarray (TMA) containing a wide range of normal and cancer tissues as well as a cell microarray consisting of a range of commonly used, well characterised human cell lines. Further results for this antibody can be found at www.proteinatlas.org
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"