Overview

  • Product nameAnti-Gata6 antibodySee all Gata6 primary antibodies ...
  • Description
    Rabbit polyclonal to Gata6
  • Tested applicationsIHC-P, WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
    Predicted to work with: Chicken, Cow, Pig
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 500 to the C-terminus of Human Gata6.

    (Peptide available as ab24410.)

  • Positive control
    • Human embryonic stem cells differentiated into mesendoderm. This antibody also gave a positive signal in D3 ES Cells Day 15 EB (Gata6 +ve) lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab22600 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/200.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 45 kDa (predicted molecular weight: 45 kDa).Can be blocked with Human Gata6 peptide (ab24410) or Human Gata6 peptide (ab24411). Abcam recommends using milk as the blocking agent.
ICC/IF 1/200.

Target

Anti-Gata6 antibody images

  • Anti-Gata6 antibody (ab22600) at 1 µg/ml + D3 ES Cells Day 15 EB (Gata6 +ve) at 10 µg

    Secondary
    IRDye 680 Conjugated Goat Anti-Rabbit IgG at 1/10000 dilution
    developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 45 kDa
    Observed band size : 45 kDa
    Additional bands at : 73 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 2 minutes
  • Image courtesy of Human Protein Atlas

    ab22600 staining Gata6 in Human stomach, with strong staining within the glandular cells. Paraffin embedded human stomach was incubated with ab22600 (1/80 dilution) for 30 mins at room temperature. Antigen retrieval was performed by heat induction in citrate buffer pH 6.

    ab22600 was tested in a tissue microarray (TMA) containing a wide range of normal and cancer tissues as well as a cell microarray consisting of a range of commonly used, well characterised human cell lines. Further results for this antibody can be found at www.proteinatlas.org

    .

References for Anti-Gata6 antibody (ab22600)

This product has been referenced in:
  • Cao S  et al. Specific gene-regulation networks during the pre-implantation development of the pig embryo as revealed by deep sequencing. BMC Genomics 15:4 (2014). IF . Read more (PubMed: 24383959) »
  • Sun L  et al. Angiotensin II induces apoptosis in intestinal epithelial cells through the AT2 receptor, GATA-6 and the Bax pathway. Biochem Biophys Res Commun 424:663-8 (2012). WB ; Human . Read more (PubMed: 22776205) »

See all 8 Publications for this product

Product Wall

Thank you for taking the time to complete our questionnaire. The details you have kindly provided will enable us to investigate this case for you and this is also helpful in our records for monitoring of quality. Reviewing this case, I would like to of...

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Thank you for contacting Abcam. From the information on the datasheet for ab22600, the antibody does recognize band ay ~45kDa and also a band about ~75kDa, therefore I am unsure what the other band that you are detecting could be. For the peptide ab244...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Tissue lysate - whole (aorta)
Loading amount 12 µg
Specification aorta
Gel Running Conditions Reduced Denaturing (10%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 27°C
Username

Abcam user community

Verified customer

Submitted Oct 29 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Cell lysate - other (differentiated mouse EBs (day 14) D3 line)
Loading amount 20 µg
Specification differentiated mouse EBs (day 14) D3 line
Gel Running Conditions Reduced Denaturing (10%)
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: rt°C
Username

Abcam user community

Verified customer

Submitted Jun 04 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (Intestine)
Specification Intestine
Fixative Formaldehyde
Antigen retrieval step Heat mediated
Permeabilization No
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Jan 09 2008

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"