Gbeta2-like antibody (ab43122)

Product Name 

Gbeta2-like antibody

Product type 

Primary antibodies

Description 

Mouse polyclonal to RACK1

Immunogen 

Recombinant fragment: SPDGSLCASG GKDGQAMLWD LNEGKHLYTL DGGDIINALC FSPNRYWLCA ATGPSIKIWD LEGKIIVDEL KQAVISTSSK AEPPQCTSLA WSADGQTLFA , corresponding to amino acids 201-300 of Mouse RACK1
BLAST 'SPDGSLCASG GKDGQAMLWD LNEGKHLYTL DGGDIINALC FSPNRYWLCA ATGPSIKIWD LEGKIIVDEL KQAVISTSSK AEPPQCTSLA WSADGQTLFA ' with or

Reacts with 

Ms

Predicted to react 
(species key)

Hu, Rat, Chk, Cow, CynMk, Pig
(Due to sequence homology)

Tested applications 
(see key)

WB

Abreviews 

Customer reviews (see key)

Read about Abreviews

Application notes 
(see key)

Recommended dilutions
WB: 1/1000. Predicted molecular weight: 35 kDa.
This antibody has been tested in Western blot against an E.coli lysate containing the partial recombinant fusion protein used as an immunogen. We have no data on detection of endogenous protein.

Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Cellular localization 

Cytoplasmic

Research areas 

Cell Biology >> Other Antibodies >> Other Antibodies
Signal Transduction >> Signaling Pathway >> G Protein Signaling >> Heterotrimeric G Proteins >> G Proteins
Signal Transduction >> Protein Phosphorylation >> Ser / Thr Kinases >> PKC
Cell Biology >> Cell Cycle >> Kinases/Phosphatases >> Cdk Regulators

Relevance 

RACK1 (receptor for activated C kinase 1) was identified through its binding to various PKC isoforms. Its main function is to recruit PKC and various other proteins to specific location to form multiprotein complexes, mediating various signal pathways.

This antibody was raised by a genetic immunization technique. Genetic immunization can be used to generate antibodies by directly delivering antigen coding DNA into the animal, rather than injecting a protein or peptide (Tang et al. PubMed: 1545867; Chambers and Johnston PubMed 12910245; Barry and Johnston PubMed: 9234514). The animal's cells produce the protein, which stimulates the animal's immune system to produce antibodies against that particular protein.
A vector coding for a partial fusion protein was used for genetic immunisation of a mouse and the resulting serum was tested in Western blot against an E.coli lysate containing that partial fusion protein.
Genetic immunization offers enormous advantages over the traditional protein based immunization method. DNA is faster, cheaper and easier to produce and can be produced by standard techniques readily amenable to automation. Furthermore, the antibodies generated by genetic immunization are usually of superior quality with regard to specificity, affinity and recognizing the native protein.

Database links 

Raised in 

Mouse

Clonality 

Polyclonal

Isotype 

IgG

Purity 

Whole antiserum

Storage buffer 

Preservative: None
Constituents: 50% Glycerol, Whole serum

Material safety datasheet (MSDS) for this product:
Glycerol MSDS

Form 

Liquid

Concentration 

Concentration not determined - why is this?

Storage instructions 

Store at -20°C. Stable for 12 months at -20°C

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