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Products:Cell Biology >> Cell Cycle >> Kinases/Phosphatases >> Cdk Regulators
Overview
Product name
Anti-RACK1 antibody
See all RACK1 products (8) ...
Description
Mouse polyclonal to RACK1
Tested applications
Cross reactivity
Reacts with
Mouse
Predicted to work with
Rat, Chicken, Cow, Human, Pig, Cynomolgus Monkey
Immunogen
Recombinant fragment: SPDGSLCASG GKDGQAMLWD LNEGKHLYTL DGGDIINALC FSPNRYWLCA ATGPSIKIWD LEGKIIVDEL KQAVISTSSK AEPPQCTSLA WSADGQTLFA , corresponding to amino acids 201-300 of Mouse RACK1
SPDGSLCASG GKDGQAMLWD LNEGKHLYTL DGGDIINALC FSPNRYWLCA ATGPSIKIWD LEGKIIVDEL KQAVISTSSK AEPPQCTSLA WSADGQTLFA
Properties
Form
Liquid
Storage instructions
Store at -20°C. Stable for 12 months at -20°C
Storage buffer
Preservative: None
Constituents: 50% Glycerol, Whole serum
Purity
Whole antiserum
Primary antibody notes
This antibody was raised by a genetic immunization technique. Genetic immunization can be used to generate antibodies by directly delivering antigen coding DNA into the animal, rather than injecting a protein or peptide (Tang et al.PubMed: 1545867; Chambers and Johnston PubMed 12910245; Barry and Johnston PubMed: 9234514). The animal's cells produce the protein, which stimulates the animal's immune system to produce antibodies against that particular protein. A vector coding for a partial fusion protein was used for genetic immunisation of a mouse and the resulting serum was tested in Western blot against an E.coli lysate containing that partial fusion protein. Genetic immunization offers enormous advantages over the traditional protein based immunization method. DNA is faster, cheaper and easier to produce and can be produced by standard techniques readily amenable to automation. Furthermore, the antibodies generated by genetic immunization are usually of superior quality with regard to specificity, affinity and recognizing the native protein.
Clonality
Polyclonal
Isotype
IgG
Research areas
Cell Biology >> Other Antibodies >> Other Antibodies
Signal Transduction >> Signaling Pathway >> G Protein Signaling >> Heterotrimeric G Proteins >> G Proteins
Signal Transduction >> Protein Phosphorylation >> Ser / Thr Kinases >> PKC
Cell Biology >> Cell Cycle >> Kinases/Phosphatases >> Cdk Regulators
Applications
Show applications key
Our Abpromise guarantee covers the use of ab43122 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB
Application notes
WB: 1/1000. Predicted molecular weight: 35 kDa.
This antibody has been tested in Western blot against an E.coli lysate containing the partial recombinant fusion protein used as an immunogen. We have no data on detection of endogenous protein.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Function
Seems to bind protein kinase C acting as an intracellular receptor to anchor the activated PKC to the cytoskeleton. May be involved in up-regulation of the activity of kinases such as PKC via binding to KRT1. Together with KRT1 and ITGB1, serves as a platform for SRC activation or inactivation. May play an important role in the developing brain and neuronal differentiation.
Sequence similarities
Belongs to the WD repeat G protein beta family.
Contains 7 WD repeats.
Domain
The WD repeats domain 5 mediates interaction with TRIM63.
Cellular localization
Cell membrane. Located on plasma membrane of neuroblastoma NMB7 cells.
Target information above from: UniProt accessionP63244
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Cell proliferation-inducing gene 21 protein antibody
- GBLP_HUMAN antibody
- Gnb2 rs1 antibody
see all
Database links
- Entrez Gene: 417044 Chicken
- Entrez Gene: 327682 Cow
- Entrez Gene: 10399 Human
- Entrez Gene: 14694 Mouse
- Entrez Gene: 397605 Pig
- Entrez Gene: 83427 Rat
- Omim: 176981 Human
- SwissProt: P63247 Chicken
see all
References for Anti-RACK1 antibody (ab43122)
ab43122 has not yet been referenced specifically in any publications.
Publishing research using ab43122? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"