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Anti-Gelsolin antibody [GS-2C4] (ab11081)

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Overview

Product name

Anti-Gelsolin antibody [GS-2C4]
See all Gelsolin products (11) ...

Description

Mouse monoclonal [GS-2C4] to Gelsolin

Tested applications

ELISA, WB, IF, IHC-Frmore details

Cross reactivity

Reacts with

Rabbit, Cow, Human, Pig

Immunogen

Full length native protein (purified) (Human).

Epitope

The antibody is directed against an epitope located on the 47 kD peptide derived from a chymotryptic cleavage of gelsolin. This peptide contains the carboxy terminal actin binding site for gelsolin.

Properties

Form

Liquid

Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Storage buffer

Preservative: 15mM Sodium Azide

Concentration

Concentration information loading...

Purity

Ascites

Clonality

Monoclonal

Clone number

GS-2C4

Isotype

IgG1

Research areas

Signal Transduction >> Cytoskeleton / ECM >> Cytoskeleton >> Microfilaments >> Actin etc >> Actin Binding Proteins

  • Immunocytochemistry/ Immunofluorescence - Gelsolin antibody [GS-2C4] (ab11081)Immunocytochemistry/ Immunofluorescence - Gelsolin antibody [GS-2C4] (ab11081) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab11081 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Target

Function

Calcium-regulated, actin-modulating protein that binds to the plus (or barbed) ends of actin monomers or filaments, preventing monomer exchange (end-blocking or capping). It can promote the assembly of monomers into filaments (nucleation) as well as sever filaments already formed. Plays a role in ciliogenesis.

Tissue specificity

Phagocytic cells, platelets, fibroblasts, nonmuscle cells, smooth and skeletal muscle cells.

Involvement in disease

Defects in GSN are the cause of amyloidosis type 5 (AMYL5) [MIM:105120]; also known as familial amyloidosis Finnish type. AMYL5 is a hereditary generalized amyloidosis due to gelsolin amyloid deposition. It is typically characterized by cranial neuropathy and lattice corneal dystrophy. Most patients have modest involvement of internal organs, but severe systemic disease can develop in some individuals causing peripheral polyneuropathy, amyloid cardiomyopathy, and nephrotic syndrome leading to renal failure.

Sequence similarities

Belongs to the villin/gelsolin family.
Contains 6 gelsolin-like repeats.

Post-translational
modifications

Phosphorylation on Tyr-86, Tyr-409, Tyr-465, Tyr-603 and Tyr-651 in vitro is induced in presence of phospholipids.

Cellular localization

Cytoplasm > cytoskeleton and Secreted.

Target information above from: UniProt accessionP06396 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • Actin depolymerizing factor antibody
  • Actin-depolymerizing factor antibody
  • ADF antibody
  • AGEL antibody
  • Amyloidosis Finnish type antibody
  • Brevin antibody
  • DKFZp313L0718 antibody
  • GELS_HUMAN antibody
  • Gelsolin antibody
  • GSN antibody
see all

Database links

Anti-Gelsolin antibody [GS-2C4] images:

  Immunocytochemistry/ Immunofluorescence - Gelsolin antibody [GS-2C4] (ab11081)

Immunocytochemistry/ Immunofluorescence - Gelsolin antibody [GS-2C4] (ab11081)

ICC/IF image of ab11081 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1% BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab11081, 1 µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue). This antibody also gave a positive IF result in Hek293, HepG2 and MCF7 cells.

References for Anti-Gelsolin antibody [GS-2C4] (ab11081)

This product has been referenced in:

  • Welander Het al. Gelsolin co-occurs with Lewy bodies in vivo and accelerates a-synuclein aggregation in vitro. Biochem Biophys Res Commun 412:32-8 (2011). WB; Human.Read more (PubMed: 21798243) »
  • Peña Eet al. Proteomic signature of thrombin-activated platelets after in vivo nitric oxide-donor treatment: coordinated inhibition of signaling (phosphatidylinositol 3-kinase-?, 14-3-3?, and growth factor receptor-bound protein 2) and cytoskeleton protein translocation. Arterioscler Thromb Vasc Biol 31:2560-9 (2011).Read more (PubMed: 21836071) »

See all 9 publications for this product

Publishing research using ab11081? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"