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Anti-Geminin antibody (ab12147)

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Overview

Product name

Anti-Geminin antibody
See all Geminin products (3) ...

Description

Rabbit polyclonal to Geminin

Specificity

This antibody detects a band representing Geminin by Western blot at 33kD on 293T cell lysate, this band can be blocked by the immunising peptide. It also detects a band at about 45kD that is not blocked by the immunising peptide and is thought to be non-specific.

Tested applications

ICC/IF, WB, IHC-Pmore details

Cross reactivity

Reacts with

Human

Does not react with

Mouse

Immunogen

Synthetic peptide conjugated to KLH derived from within residues 50 - 150 of Human Geminin.

Properties

Form

Liquid

Storage instructions

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Storage buffer

pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Note: Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.

Concentration

Concentration information loading...

Purity

Immunogen affinity purified

Clonality

Polyclonal

Isotype

IgG

  • Western blot Western blot  image (enlarge)

  • Immunocytochemistry/ Immunofluorescence - Geminin antibody (ab12147)Immunocytochemistry/ Immunofluorescence - Geminin antibody (ab12147) image (enlarge)

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Geminin antibody (ab12147)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Geminin antibody (ab12147) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab12147 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Target

Function

Inhibits DNA replication by preventing the incorporation of MCM complex into prereplication complex (pre-RC). It is degraded during the mitotic phase of the cell cycle. Its destruction at the metaphase-anaphase transition permits replication in the succeeding cell cycle.

Sequence similarities

Belongs to the geminin family.

Developmental stage

Absent during G1 phase, accumulates during S, G2, and M phases, and disappears at the time of the metaphase-anaphase transition.

Target information above from: UniProt accessionO75496 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • DNA replication inhibitor antibody
  • Gem antibody
  • GEMI_HUMAN antibody
  • Geminin antibody
  • Geminin DNA replication inhibitor antibody
  • GMNN antibody
  • OTTHUMP00000039393 antibody
  • RP3 369A17.3 antibody
see all

Anti-Geminin antibody images:

  Western blot

Western blot

All lanes : Anti-Geminin antibody (ab12147) at 1/500 dilution

Lane 1 : 293 lysate
Lane 2 : 293 lysate with Geminin peptide (ab12408) at 1 µg/ml

Lysates/proteins at 5 µg/ml per lane.

developed using the ECL technique

Performed under reducing conditions.

Predicted band size : 23.5 kDa
Observed band size : 33 kDa (why is the actual band size different from the predicted?)


The lower band represents Geminin (33 kDa - predicted molecular weight 23 kDa), and is blocked by the immunising peptide, the upper band is likely to be non-specific as it is not blocked by the peptide.

  Immunocytochemistry/ Immunofluorescence - Geminin antibody (ab12147)

Immunocytochemistry/ Immunofluorescence - Geminin antibody (ab12147)

ab12147 (1/400) staining geminin in assynchronous HeLa cells. Cells were fixed in paraformaldehyde and counterstained with DAPI in order to highlight the nucleus. Secondary antibody: goat anti-rabbit IgG conjugated to Cy3. Please refer to abreview for further details.

This image is courtesy of an Abreview submitted by Dr Kirk McManus

See Abreview

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Geminin antibody (ab12147)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Geminin antibody (ab12147)

Image courtesy of Human Protein Atlas

ab12147 staining in human testis, showing staining of the cells in the ductus seminiferus (in brown). Paraffin embedded testis tissue was incubated with ab12147 (1:175 dilution) for 30 mins at room temperature. Antigen retrieval was performed by heat induction in citrate buffer pH 6.

ab12147 was tested in a tissue microarray (TMA) containing a wide range of normal and cancer tissues as well as a cell microarray consisting of a range of commonly used, well characterised human cell lines. Further results for this antibody can be found at www.proteinatlas.org

.

  Western blot - Geminin antibody (ab12147)

Western blot - Geminin antibody (ab12147)

Lane 1 : Anti-Geminin antibody (ab12147) at 1 µg/ml
Lane 2 : Anti-6X His tag® antibody - ChIP Grade (ab9108) at 1 µg/ml

Lane 1 : Geminin protein (His tag) (ab40570)
Lane 2 : Geminin protein (His tag) (ab40570)

Lysates/proteins at 0.1 µg per lane.

Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

Performed under reducing conditions.

Predicted band size : 23.5 kDa
Observed band size : 32 kDa (why is the actual band size different from the predicted?)

  Western blot - Geminin antibody (ab12147)

Western blot - Geminin antibody (ab12147)

Anti-Geminin antibody (ab12147) at 1/500 dilution + Cell lysate prepared from human HeLa nuclear extract at 50 µg

Secondary
HRP-conjugated goat poyclonal to rabbit IgG at 1/5000 dilution
developed using the ECL technique

Performed under reducing conditions.

Predicted band size : 23.5 kDa
Observed band size : 33 kDa (why is the actual band size different from the predicted?)
Additional bands at : 95 kDa. We are unsure as to the identity of these extra bands.

Exposure time : 10 minutes

This image is a courtesy of Anonymous Abreview

See Abreview

References for Anti-Geminin antibody (ab12147)

This product has been referenced in:

  • Hochegger Het al. An essential role for Cdk1 in S phase control is revealed via chemical genetics in vertebrate cells. J Cell Biol 178:257-68 (2007). WB; Human.Read more (PubMed: 17635936) »

See 1 publication for this product

Publishing research using ab12147? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"