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Immunocytochemistry/ Immunofluorescence - Geminin antibody (ab12147)
Immunocytochemistry/ Immunofluorescence - Geminin antibody (ab12147)
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Geminin antibody (ab12147)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Geminin antibody (ab12147)
(enlarge)


Product Name 

Geminin antibody

 

See all Geminin antibodies (3)...

Product type 

Primary antibodies

Description 

Rabbit polyclonal to Geminin

Immunogen 

Synthetic peptide conjugated to KLH derived from within residues 50 - 150 of Human Geminin.

Reacts with 

Hu

Does not react with 
(species key)

Ms

Specificity 

This antibody detects a band representing Geminin by Western blot at 33kD on 293T cell lysate, this band can be blocked by the immunising peptide. It also detects a band at about 45kD that is not blocked by the immunising peptide and is thought to be non-specific.

Tested applications 
(see key)

ICC/IF, IHC-P, WB


Abreviews 

Customer reviews
(see key)


Read about Abreviews
(Read all Abreviews)

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• ICC/IF

2 Abreviews

- average rating: 5 stars (out of 5)5 stars (out of 5)5 stars (out of 5)5 stars (out of 5)5 stars (out of 5)

• WB

4 Abreviews

- average rating: 4 stars (out of 5)4 stars (out of 5)4 stars (out of 5)4 stars (out of 5)4 stars (out of 5)


WB
(read Abreview)


WB
(read Abreview)


ICC/IF
(read Abreview)


WB
(read Abreview)


Application notes 
(see key)

Recommended dilutions
ICC/IF: 1/400.
IHC-P: Use at an assay dependent dilution. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
WB: 1/500. Detects a band of approximately 33 kDa (predicted molecular weight: 23.5 kDa).


Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Cellular localization 

Nuclear

Research areas 

Chromatin and Nuclear Signaling >> DNA / RNA >> DNA Synthesis >> Other

Relevance 

Geminin inhibits DNA replication by preventing the incorporation of MCM complex into prereplication complex (pre-RC). It is degraded during the mitotic phase of the cell cycle. It has a destruction box sequence (RRTLKVIQP) and is ubiquitinated by the anaphase promoting complex in vitro. Its destruction at the metaphase-anaphase transition permits replication in the succeeding cell cycle. Geminin interacts tightly with CDT1, a replication initiation factor necessary for MCM loading.

 

Alternative names Geminin antibody (ab12147)

Database links 

The links below go to external sites and will open in a new browser window

Entrez Gene

    

51053    (Human)

GeneCard

    

GC06P024883    (Human)

Omim

    

602842    (Human)

SwissProt

    

O75496    (Human)

Unigene

    

234896    (Human)

Raised in 

Rabbit

Clonality 

Polyclonal

Isotype 

IgG

Purity 

Immunogen affinity purified

Storage buffer 

Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4

Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS

Form 

Liquid

Concentration 

Batch dependent within range: 0.10 - 0.50 mg/ml

Concentration of lot no.     is  

Concentration not available for this lot.

Find concentration of your lot :

Storage instructions 

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

 

At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this Geminin antibody is on this datasheet. But please do contact us if you would like any reassurance!


See below for Geminin antibody images, references, products related to ab12147 and other tools.

 

Geminin antibody images:

Product image  Western blot

Western blot

All lanes : Geminin antibody (ab12147) at 1/500 dilution

Lane 1 : 293 lysate
Lane 2 : 293 lysate with Geminin peptide (ab12408) at 1 µg/ml

Lysates/proteins at 5 µg/ml per lane.

developed using the ECL technique

Performed under reducing conditions.

Predicted band size : 23.5 kDa
Observed band size : 33 kDa (why is the actual band size different from the predicted?)


The lower band represents Geminin (33 kDa - predicted molecular weight 23 kDa), and is blocked by the immunising peptide, the upper band is likely to be non-specific as it is not blocked by the peptide.

 

Product image  Immunocytochemistry/ Immunofluorescence - Geminin antibody (ab12147)

Immunocytochemistry/ Immunofluorescence - Geminin antibody (ab12147)

ab12147 (1/400) staining geminin in assynchronous HeLa cells. Cells were fixed in paraformaldehyde and counterstained with DAPI in order to highlight the nucleus. Secondary antibody: goat anti-rabbit IgG conjugated to Cy3. Please refer to abreview for further details.

This image is courtesy of an Abreview submitted by Dr Kirk McManus

See Abreview

 

Product image  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Geminin antibody (ab12147)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Geminin antibody (ab12147)

Image courtesy of Human Protein Atlas

ab12147 staining in human testis, showing staining of the cells in the ductus seminiferus (in brown). Paraffin embedded testis tissue was incubated with ab12147 (1:175 dilution) for 30 mins at room temperature. Antigen retrieval was performed by heat induction in citrate buffer pH 6.

ab12147 was tested in a tissue microarray (TMA) containing a wide range of normal and cancer tissues as well as a cell microarray consisting of a range of commonly used, well characterised human cell lines. Further results for this antibody can be found at www.proteinatlas.org

.

 

Product image  Western blot - Geminin antibody (ab12147)

Western blot - Geminin antibody (ab12147)

Lane 1 : Geminin antibody (ab12147) at 1 µg/ml
Lane 2 : 6X His tag® antibody - ChIP Grade (ab9108) at 1 µg/ml

Lane 1 : Geminin protein (His tag) (ab40570)
Lane 2 : Geminin protein (His tag) (ab40570)

Lysates/proteins at 0.1 µg per lane.

Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

Performed under reducing conditions.

Predicted band size : 23.5 kDa
Observed band size : 32 kDa (why is the actual band size different from the predicted?)

 

References for Geminin antibody (ab12147)

Specific publication references   This product has been used in:

Hochegger H et al. An essential role for Cdk1 in S phase control is revealed via chemical genetics in vertebrate cells. J Cell Biol 178:257-68 (2007). WB; Human. PubMed: 17635936

If you publish research using ab12147 please let us know so that we can cite the reference on this datasheet.

 

Search PubMed (MEDLINE) for references to Geminin

 

Geminin antibody - more information

Customer reviews (feedback) regarding Geminin antibody

Customer FAQs regarding Geminin antibody

Protocols for Geminin antibody

Price and availability of products related to Geminin antibody

 

Geminin antibody - related products:

Products for Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

Slides

Products for Western blot

Loading controls

Lysates

 

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Other Geminin Antibodies

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Geminin antibody - other tools:

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