Anti-Geminin antibody (ab187553)
Key features and details
- Rabbit polyclonal to Geminin
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Geminin antibody
See all Geminin primary antibodies -
Description
Rabbit polyclonal to Geminin -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant full length protein corresponding to Human Geminin aa 1 to the C-terminus.
Database link: O75496 -
Positive control
- SW480, 22RV-1, HL60, HepG2, MCF7 and K562 cell line extracts.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol, 49% PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab187553 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
1/50 - 1/200.
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WB |
1/500 - 1/2000. Predicted molecular weight: 24 kDa.
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Notes |
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IHC-P
1/50 - 1/200. |
WB
1/500 - 1/2000. Predicted molecular weight: 24 kDa. |
Target
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Function
Inhibits DNA replication by preventing the incorporation of MCM complex into prereplication complex (pre-RC). It is degraded during the mitotic phase of the cell cycle. Its destruction at the metaphase-anaphase transition permits replication in the succeeding cell cycle. -
Sequence similarities
Belongs to the geminin family. -
Developmental stage
Absent during G1 phase, accumulates during S, G2, and M phases, and disappears at the time of the metaphase-anaphase transition. - Information by UniProt
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Database links
- Entrez Gene: 51053 Human
- Omim: 602842 Human
- SwissProt: O75496 Human
- Unigene: 234896 Human
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Alternative names
- DNA replication inhibitor antibody
- Gem antibody
- GEMI_HUMAN antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human liver cancer tissue labelling Geminin with ab187553 at 1/200. Magnification: 200x.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast cancer tissue labelling Geminin with ab187553 at 1/200. Magnification: 200x.
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All lanes : Anti-Geminin antibody (ab187553) at 1/500 dilution
Lane 1 : SW480 cell line extract
Lane 2 : 22RV-1 cell line extract
Lane 3 : HL60 cell line extract
Lane 4 : HepG2 cell line extract
Lane 5 : MCF7 cell line extract
Lane 6 : K562 cell line extract
Predicted band size: 24 kDa
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab187553 has not yet been referenced specifically in any publications.