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If your product does not perform as described on this datasheet, we will refund or replace your product...
Read our guarantee »Publishing research using ab12408? Please let us know so that we can cite the reference in this datasheet
ab12408 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Western blot
All lanes : Anti-Geminin antibody (ab12147) at 1/500 dilution
Lane 1 : 293 lysate
Lane 2 : 293 lysate with
Lysates/proteins at 5 µg/ml per lane.
developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 23.5 kDa
Observed band size : 33 kDa (why is the actual band size different from the predicted?)
The lower band represents Geminin (33 kDa - predicted molecular weight 23 kDa), and is blocked by the immunising peptide, the upper band is likely to be non-specific as it is not blocked by the peptide.
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