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If your product does not perform as described on this datasheet, we will refund or replace your product...
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Western blot
All lanes : Anti-Geminin antibody (ab12147) at 1/500 dilution
Lane 1 : 293 lysate
Lane 2 : 293 lysate with
Lysates/proteins at 5 µg/ml per lane.
developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 23.5 kDa
Observed band size : 33 kDa (why is the actual band size different from the predicted?)
The lower band represents Geminin (33 kDa - predicted molecular weight 23 kDa), and is blocked by the immunising peptide, the upper band is likely to be non-specific as it is not blocked by the peptide.
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