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Product Name
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Geminin peptide
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Product type
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Proteins and Peptides
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Peptide
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Synthetic peptide derived from within residues 50 - 150 of Human Geminin. .
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Research areas
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Chromatin and Nuclear Signaling >> DNA / RNA >> DNA Synthesis >> Other
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Relevance
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Geminin inhibits DNA replication by preventing the incorporation of MCM complex into prereplication complex (pre-RC). It is degraded during the mitotic phase of the cell cycle. It has a destruction box sequence (RRTLKVIQP) and is ubiquitinated by the anaphase promoting complex in vitro. Its destruction at the metaphase-anaphase transition permits replication in the succeeding cell cycle. Geminin interacts tightly with CDT1, a replication initiation factor necessary for MCM loading.
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Geminin peptide (ab12408) |
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Storage buffer
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Preservative: None
Constituents: 0.001% Tween 20, HEPES, 2mM EDTA, 150mM Sodium chloride
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Form
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Liquid
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Concentration
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1.000 mg/ml
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this Geminin peptide is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for Geminin peptide images, references, products related to ab12408 and other tools.
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Geminin peptide images:
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 Western blot
All lanes : Geminin antibody (ab12147) at 1/500 dilution
Lane 1 : 293 lysate
Lane 2 : 293 lysate with Geminin peptide (ab12408) at 1 µg/ml
Lysates/proteins at 5 µg/ml per lane.
developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 23.5 kDa
Observed band size : 33 kDa (why is the actual band size different from the predicted?)
The lower band represents Geminin (33 kDa - predicted molecular weight 23 kDa), and is blocked by the immunising peptide, the upper band is likely to be non-specific as it is not blocked by the peptide.
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Search PubMed (MEDLINE) for references to Geminin
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Geminin peptide - more information
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Customer reviews (feedback) regarding Geminin peptide |
Customer FAQs regarding Geminin peptide |
Protocols for Geminin peptide |
Price and availability of products related to Geminin peptide |
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Geminin peptide - related products:
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Geminin peptide - other tools:
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Contact Abcam with a Technical Enquiry about Geminin peptide |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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