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Geminin peptide (ab12408)

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Overview

Product name

Geminin peptide
See all Geminin products (3) ...

Protein description

Synthetic peptide derived from within residues 50 - 150 of Human Geminin. (Note: the amino acid sequence is proprietary). 

Properties

Form

Liquid

Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Storage buffer

Information available upon request.

Concentration

Concentration information loading...

Research areas

Epigenetics and Nuclear Signaling >> DNA / RNA >> DNA Synthesis >> Other

Applications

Application notes

This peptide can be used with studies using ab12147.

Protein info

Function

Inhibits DNA replication by preventing the incorporation of MCM complex into prereplication complex (pre-RC). It is degraded during the mitotic phase of the cell cycle. Its destruction at the metaphase-anaphase transition permits replication in the succeeding cell cycle.

Sequence similarities

Belongs to the geminin family.

Developmental stage

Absent during G1 phase, accumulates during S, G2, and M phases, and disappears at the time of the metaphase-anaphase transition.

Target information above from: UniProt accessionO75496 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Geminin peptide images:

  Western blot

Western blot

All lanes : Anti-Geminin antibody (ab12147) at 1/500 dilution

Lane 1 : 293 lysate
Lane 2 : 293 lysate with Geminin peptide (ab12408) at 1 µg/ml

Lysates/proteins at 5 µg/ml per lane.

developed using the ECL technique

Performed under reducing conditions.

Predicted band size : 23.5 kDa
Observed band size : 33 kDa (why is the actual band size different from the predicted?)


The lower band represents Geminin (33 kDa - predicted molecular weight 23 kDa), and is blocked by the immunising peptide, the upper band is likely to be non-specific as it is not blocked by the peptide.

References for Geminin peptide (ab12408)

ab12408 has not yet been referenced specifically in any publications.

Publishing research using ab12408? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"