Loading...
You have changed your country from
to
. Please be aware that this will change the currency in the purchasing process.
Products:Epigenetics and Nuclear Signaling >> DNA / RNA >> DNA Synthesis >> Other
Overview
Product name
Geminin protein (His tag)
See all Geminin products (3) ...
Protein description
Recombinant full length protein: Human Geminin fused to His-Tag at N-terminus.
Expression host
E. coli
Properties
Purity
>95% by SDS-PAGE
Form
Liquid
Storage instructions
Aliquot and store at -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.002% Sodium Azide
Constituents: 3mM Sodium chloride, 2.5mM Imidazole, 10mM Tris, pH 8.0
Concentration
Concentration information loading...
Research areas
Epigenetics and Nuclear Signaling >> DNA / RNA >> DNA Synthesis >> Other
-
SDS-PAGE - Geminin protein (ab40570)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab40570 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
MS
- 1 Image
SDS-PAGE
Application notes
MS: Use at an assay dependent dilution.
SDS-PAGE: Use 1µg.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Protein info
Function
Inhibits DNA replication by preventing the incorporation of MCM complex into prereplication complex (pre-RC). It is degraded during the mitotic phase of the cell cycle. Its destruction at the metaphase-anaphase transition permits replication in the succeeding cell cycle.
Sequence similarities
Belongs to the geminin family.
Developmental stage
Absent during G1 phase, accumulates during S, G2, and M phases, and disappears at the time of the metaphase-anaphase transition.
Target information above from: UniProt accessionO75496
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Geminin protein (His tag) images:
SDS-PAGE - Geminin protein (ab40570)
Analysis of GMNN (Geminin) Recombinant Protein (1µg).
4-20% SDS gradient gel. Coomassie blue staining.
References for Geminin protein (His tag) (ab40570)
ab40570 has not yet been referenced specifically in any publications.
Publishing research using ab40570? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"