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Products:Epigenetics and Nuclear Signaling >> DNA / RNA >> DNA Synthesis >> Other
Overview
Product name
Geminin protein (His tag)
See all Geminin products (3) ...
Protein description
Recombinant full length Human Geminin fused to His-tag at N-terminus; amino acids 1-209 (245 amino acids), 27.7kDa. Swiss ID = O75496
Expression host
E. coli
Properties
Purity
>95% by SDS-PAGE
Purification notes
ab86447 is purified using conventional chromatography techniques.
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: 10% Glycerol, 20mM Tris, 100mM Sodium chloride, pH 8.0
Concentration
Concentration information loading...
Sequence notes
MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSMNPS MKQKQEEIKE NIKNSSVPRR TLKMIQPSAS GSLVGRENEL SAGLSKRKHR NDHLTSTTSS PGVIVPESSE NKNLGGVTQE SFDLMIKENP SSQYWKEVAE KRRKALYEAL KENEKLHKEI EQKDNEIARL KKENKELAEV AEHVQYMAEL IERLNGEPLD NFESLDNQEF DSEEETVEDS LVEDSEIGTC AEGTVSSSTD AKPCI
Research areas
Epigenetics and Nuclear Signaling >> DNA / RNA >> DNA Synthesis >> Other
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SDS-PAGE - Geminin protein (His tag) (ab86447)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab86447 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
SDS-PAGE: Use at an assay dependent dilution.
Protein info
Function
Inhibits DNA replication by preventing the incorporation of MCM complex into prereplication complex (pre-RC). It is degraded during the mitotic phase of the cell cycle. Its destruction at the metaphase-anaphase transition permits replication in the succeeding cell cycle.
Sequence similarities
Belongs to the geminin family.
Developmental stage
Absent during G1 phase, accumulates during S, G2, and M phases, and disappears at the time of the metaphase-anaphase transition.
Target information above from: UniProt accessionO75496
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Geminin protein (His tag) images:
References for Geminin protein (His tag) (ab86447)
ab86447 has not yet been referenced specifically in any publications.
Publishing research using ab86447? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"