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SDS-PAGE - Geminin protein (His tag) (ab86447)
SDS-PAGE - Geminin protein (His tag) (ab86447)
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Product Name 

Geminin protein (His tag)

Product type 

Proteins and Peptides

Peptide 

Recombinant full length Human Geminin fused to His-tag at N-terminus; amino acids 1-209 (245 amino acids), 27.7kDa. Swiss ID = O75496

Tested applications 
(see key)

SDS-PAGE

Application notes 
(see key)

Recommended dilutions
SDS-PAGE: Use at an assay dependent dilution.


Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Research areas 

Chromatin and Nuclear Signaling >> DNA / RNA >> DNA Synthesis >> Other

Relevance 

Geminin inhibits DNA replication by preventing the incorporation of MCM complex into prereplication complex (pre-RC). It is degraded during the mitotic phase of the cell cycle. It has a destruction box sequence (RRTLKVIQP) and is ubiquitinated by the anaphase promoting complex in vitro. Its destruction at the metaphase-anaphase transition permits replication in the succeeding cell cycle. Geminin interacts tightly with CDT1, a replication initiation factor necessary for MCM loading.

 

Alternative names Geminin protein (His tag) (ab86447)

Purity 

>95% by SDS-PAGE

Storage buffer 

Preservative: None
Constituents: 10% Glycerol, 20mM Tris, 100mM Sodium chloride, pH 8.0

Material safety datasheet (MSDS) for this product:
Glycerol MSDS

Purification notes 

ab86447 is purified using conventional chromatography techniques.

Form 

Liquid

Concentration 

1.000 mg/ml

Storage instructions 

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.

Expression system 

E. coli

Sequence 

MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSMNPS MKQKQEEIKE NIKNSSVPRR TLKMIQPSAS GSLVGRENEL SAGLSKRKHR NDHLTSTTSS PGVIVPESSE NKNLGGVTQE SFDLMIKENP SSQYWKEVAE KRRKALYEAL KENEKLHKEI EQKDNEIARL KKENKELAEV AEHVQYMAEL IERLNGEPLD NFESLDNQEF DSEEETVEDS LVEDSEIGTC AEGTVSSSTD AKPCI

 

At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this Geminin protein (His tag) is on this datasheet. But please do contact us if you would like any reassurance!


See below for Geminin protein (His tag) images, references, products related to ab86447 and other tools.

 

Geminin protein (His tag) images:

Product image  SDS-PAGE - Geminin protein (His tag) (ab86447)

SDS-PAGE - Geminin protein (His tag) (ab86447)

15% SDS-PAGE showing ab86447.

 
 

Search PubMed (MEDLINE) for references to Geminin

 

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Geminin protein (His tag) - related products:

 

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Rabbit polyclonal to Geminin

 

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Geminin protein (His tag) - other tools:

Contact Abcam with a Technical Enquiry about Geminin protein (His tag)

 

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