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SDS-PAGE - Geminin protein (His tag) (ab86447)
(enlarge)
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Product Name
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Geminin protein (His tag)
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Product type
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Proteins and Peptides
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Peptide
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Recombinant full length Human Geminin fused to His-tag at N-terminus; amino acids 1-209 (245 amino acids), 27.7kDa. Swiss ID = O75496
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Tested applications
(see key)
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SDS-PAGE
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Application notes
(see key)
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Recommended dilutions
SDS-PAGE: Use at an assay dependent dilution.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Research areas
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Chromatin and Nuclear Signaling >> DNA / RNA >> DNA Synthesis >> Other
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Relevance
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Geminin inhibits DNA replication by preventing the incorporation of MCM complex into prereplication complex (pre-RC). It is degraded during the mitotic phase of the cell cycle. It has a destruction box sequence (RRTLKVIQP) and is ubiquitinated by the anaphase promoting complex in vitro. Its destruction at the metaphase-anaphase transition permits replication in the succeeding cell cycle. Geminin interacts tightly with CDT1, a replication initiation factor necessary for MCM loading.
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Purity
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>95% by SDS-PAGE
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Storage buffer
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Preservative: None
Constituents: 10% Glycerol, 20mM Tris, 100mM Sodium chloride, pH 8.0 Material safety datasheet (MSDS) for this product:
Glycerol MSDS
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Purification notes
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ab86447 is purified using conventional chromatography techniques.
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Form
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Liquid
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Concentration
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1.000 mg/ml
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
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Expression system
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E. coli
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Sequence
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MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSMNPS MKQKQEEIKE NIKNSSVPRR TLKMIQPSAS GSLVGRENEL SAGLSKRKHR NDHLTSTTSS PGVIVPESSE NKNLGGVTQE SFDLMIKENP SSQYWKEVAE KRRKALYEAL KENEKLHKEI EQKDNEIARL KKENKELAEV AEHVQYMAEL IERLNGEPLD NFESLDNQEF DSEEETVEDS LVEDSEIGTC AEGTVSSSTD AKPCI
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this Geminin protein (His tag) is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for Geminin protein (His tag) images, references, products related to ab86447 and other tools.
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Geminin protein (His tag) images:
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 SDS-PAGE - Geminin protein (His tag) (ab86447)
15% SDS-PAGE showing ab86447.
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Search PubMed (MEDLINE) for references to Geminin
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