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Generic Caspase Activity Assay Kit (Fluorometric - Green) (ab112130)

Q&A (2)

Overview

  • Product nameGeneric Caspase Activity Assay Kit (Fluorometric - Green)See all Caspase kits ...
  • Sample type
    Adherent cells, Suspension cells
  • Assay typeDirect
  • Species reactivity
    Reacts with: Human
  • Product overview

    Abcam's Activity Assay kits are a set of tools for monitoring cellular functions. The activation of caspase is widely accepted as a reliable indicator for cell apoptosis. Most caspases have substrate selectivity for the peptide sequence Val-Ala-Asp (VAD). ab112130 uses TF2-VAD-FMK as a fluorescent indicator for most caspase activities. The cell permeable and nontoxic TF2-VAD-FMK irreversibly binds to activated caspase-1, -3, -4, -5, -6, -7, -8 and -9 in apoptotic cells. Once bound to caspases, the fluorescent reagent is retained inside the cell. The binding event prevents the caspases from further catalysis but will not stop apoptosis from proceeding. Within 15 minutes incubation, it starts to react with active caspase enzymes.

    ab112130 provides all the essential components with an optimized assay protocol. It is designed to detect cell apoptosis by measuring generic activation of caspases (caspase-1, -3, -4, -5, -6, -7, -8 and -9) in live cells.

    ab112130 is used for either the quantification of most activated caspase activities in apoptotic cells or screening of caspase inhibitors. TF2-VAD-FMK, the green label reagent, allows for direct detection of activated caspases in apoptotic cells by a flow cytometer at Ex/Em = 488/520 nm.

    Visit our FAQs page for tips and troubleshooting.

  • Notes

    ab112130 should be stored dessicated.

  • Tested applicationsFlow Cyt, Functional Studies more details

Properties

  • RelevanceCaspases are members of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce 2 subunits, large and small, that dimerize to form the active enzyme.
  • Cellular localizationCytoplasmic

    • Applications

    Our Abpromise guarantee covers the use of ab112130 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    Flow Cyt Flow Cyt
    Functional Studies FuncS

    Generic Caspase Activity Assay Kit (Fluorometric - Green) images

    • Flow Cytometry - Generic Caspase Activity Assay Kit (Fluorometric - Green) (ab112130)
      Flow Cytometry - Generic Caspase Activity Assay Kit (Fluorometric - Green) (ab112130)
      The increase in TF2-VAD-FMK (Component A) fluorescence intensity with the addition of Camptothecin in Jurkat cells.
      Jurkat cells were untreated (Blue) or with 20 μM camptothecin (Pink) in a 37 °C, 5 % CO2 incubator for 4-5 hours, and then dye loaded with TF2-VAD-FMK for 1 hour.

    Protocols

    References for Generic Caspase Activity Assay Kit (Fluorometric - Green) (ab112130)

    ab112130 has not yet been referenced specifically in any publications.

    Publishing research using ab112130 ? Please let us know so that we can cite the reference in this datasheet

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    However, If i fix the cell sample using 70% alcohol such as cell
    stained by PI to runing cell cycle then the sample just can store at 4
    oC for 24hour, right?

    Thank you for your reply.

    You are correct that you can your cells for up to 24hours at 4C after they have been fixed. After the cells have been fixed. The kit is intended to be used on live cells.

    Please let me know if there is anythin...

    Read More

    Inthe protocolofthis productionwhichpage 8, the F step. For cell fixation, how long can it bestored at -20 or -80 oC?
    I need the important information, please help me.
    thanks a lot!

    Thank you forcontacting Abcam.

    Youcan store your samples at 4C for up to 24hours, but you cannot freeze them.

    Please let me know if there is anything else I can help you with.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"