Overview

  • Product nameAnti-Gephyrin antibodySee all Gephyrin primary antibodies ...
  • Description
    Rabbit polyclonal to Gephyrin
  • Tested applicationsIHC-P, ICC, IHC-FoFr, WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
    Predicted to work with: Chicken, Cow
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Mouse Gephyrin.

    (Peptide available as ab32204.)

  • Positive control
    • This antibody gave a positive signal in the following lysates: Brain (Mouse), Brain 0 Day (Mouse), NIH 3T3 (Mouse), MEF-1 (Mouse) and PC12 (Rat).

Properties

Applications

Our Abpromise guarantee covers the use of ab25784 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration.
ICC Use at an assay dependent concentration.
IHC-FoFr 1/300.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 93 kDa (predicted molecular weight: 83 kDa).Can be blocked with Mouse Gephyrin peptide (ab32204) or Mouse Gephyrin peptide (ab32205).
ICC/IF Use a concentration of 1 µg/ml.

Target

  • FunctionMicrotubule-associated protein involved in membrane protein-cytoskeleton interactions. It is thought to anchor the inhibitory glycine receptor (GLYR) to subsynaptic microtubules (By similarity). Catalyzes two steps in the biosynthesis of the molybdenum cofactor. In the first step, molybdopterin is adenylated. Subsequently, molybdate is inserted into adenylated molybdopterin and AMP is released.
  • PathwayCofactor biosynthesis; molybdopterin biosynthesis.
  • Involvement in diseaseDefects in GPHN are the cause of molybdenum cofactor deficiency type C (MOCOD type C) [MIM:252150]. MOCOD type C is an autosomal recessive disease which leads to the pleiotropic loss of all molybdoenzyme activities and is characterized by severe neurological damage, neonatal seizures and early childhood death.
    Defects in GPHN are a cause of startle disease (STHE) [MIM:149400]; also known as hyperekplexia. STHE is a genetically heterogeneous neurologic disorder characterized by muscular rigidity of central nervous system origin, particularly in the neonatal period, and by an exaggerated startle response to unexpected acoustic or tactile stimuli.
  • Sequence similaritiesIn the N-terminal section; belongs to the moaB/mog family.
    In the C-terminal section; belongs to the moeA family.
  • Cellular localizationCell junction > synapse. Cell junction > synapse > postsynaptic cell membrane. Cytoplasm > cytoskeleton. Cytoplasmic face of glycinergic postsynaptic membranes.
  • Information by UniProt
  • Database links
  • Alternative names
    • Domain E antibody
    • Domain G antibody
    • GEPH antibody
    • GEPH_HUMAN antibody
    • GPH antibody
    • GPHN antibody
    • GPHRYN antibody
    • KIAA1385 antibody
    • Molybdopterin molybdenumtransferase antibody
    • MPT adenylyltransferase antibody
    • MPT Mo-transferase antibody
    see all

Anti-Gephyrin antibody images

  • All lanes : Anti-Gephyrin antibody (ab25784) at 1 µg/ml

    Lane 1 : Brain (Mouse) Tissue Lysate
    Lane 2 : Brain (Mouse) Tissue Lysate - normal tissue, 0 days old (ab7188)
    Lane 3 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate (ab7179)
    Lane 4 : MEF1 (Mouse embryonic fibroblast cell line) Whole Cell Lysate
    Lane 5 : PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Performed under reducing conditions.

    Predicted band size : 83 kDa
    Observed band size : 93 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 26 kDa. We are unsure as to the identity of these extra bands.
  • Immunohistochemistical detection of Gephyrin using antibody ab25784 on rat brain PFA perfusion-fixed free floating sections. Antigen retrieval step: None. Primary Antibody Dilution 1/300 incubated for 18 hours @ 20°C in PBS + 0.3 % Triton X100. Secondary Antibody: Goat anti-rabbit Alexa Fluor® 488 (1/1000). The submitted image is of a 30um rat brain section; a thinly punctuated staining was observed in the adult rat brain. The pictures show the staining obtained in the striatum (A) using the X20 objective and in the cerebral cortex using the X40 objective (B). The tissues were fixed with 4% PFA and later postfixed overnight in the same fixative. They were cryoprotected in 30% sucrose and cut using a cryostat.

    See Abreview

  • ICC/IF image of ab25784 stained PC12 cells. The cells were 4% formaldeyhde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab25784, 1µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 100% methanol fixed (5 min) XXX cells at 1µg/ml.

References for Anti-Gephyrin antibody (ab25784)

This product has been referenced in:
  • Ke MT  et al. SeeDB: a simple and morphology-preserving optical clearing agent for neuronal circuit reconstruction. Nat Neurosci 16:1154-61 (2013). Read more (PubMed: 23792946) »
  • Duarte JM  et al. Caffeine consumption prevents diabetes-induced memory impairment and synaptotoxicity in the hippocampus of NONcZNO10/LTJ mice. PLoS One 7:e21899 (2012). WB ; Mouse . Read more (PubMed: 22514596) »

See all 2 Publications for this product

Product Wall

Thank you for contacting us. I am sorry that the vial you received did not contain the full amount of the product and I apologize for the inconvenience. I have issued a free of charge replacement vial with the order number of xxxx. Ple...

Read More
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (Caco-2)
Specification Caco-2
Fixative Formaldehyde
Permeabilization No
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Oct 07 2010

Application Immunocytochemistry
Sample Human Cultured Cells (Caco-2)
Specification Caco-2
Fixative Formaldehyde
Permeabilization No
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Oct 07 2010

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (Human Tumor - Colon)
Specification Human Tumor - Colon
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: DAKO target retrieval solution
Permeabilization No
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Oct 07 2010

Application Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample Rat Tissue sections (Brain sections)
Specification Brain sections
Fixative Paraformaldehyde
Antigen retrieval step None
Permeabilization No
Username

Dr. Sophie Pezet

Verified customer

Submitted May 10 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"