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Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Mouse Gephyrin.
(Peptide available as ab32204.)
Our Abpromise guarantee covers the use of ab25784 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use at an assay dependent concentration.|
|ICC||Use at an assay dependent concentration.|
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 93 kDa (predicted molecular weight: 83 kDa).Can be blocked with Mouse Gephyrin peptide (ab32204) or Mouse Gephyrin peptide (ab32205).|
|ICC/IF||Use a concentration of 1 µg/ml.|
Immunohistochemistical detection of Gephyrin using antibody ab25784 on rat brain PFA perfusion-fixed free floating sections. Antigen retrieval step: None. Primary Antibody Dilution 1/300 incubated for 18 hours @ 20°C in PBS + 0.3 % Triton X100. Secondary Antibody: Goat anti-rabbit Alexa Fluor® 488 (1/1000). The submitted image is of a 30um rat brain section; a thinly punctuated staining was observed in the adult rat brain. The pictures show the staining obtained in the striatum (A) using the X20 objective and in the cerebral cortex using the X40 objective (B). The tissues were fixed with 4% PFA and later postfixed overnight in the same fixative. They were cryoprotected in 30% sucrose and cut using a cryostat.
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