Anti-Gephyrin antibody (ab32206)

Overview

• Product nameAnti-Gephyrin antibodySee all Gephyrin primary antibodies ...
• Description
  Rabbit polyclonal to Gephyrin
• Tested applicationsICC/IF, IHC-FoFr, WB more details
• Species reactivity
  Reacts with: Mouse, Rat
  Predicted to work with: Chicken, Human, Xenopus laevis
• Immunogen

  Synthetic peptide conjugated to KLH derived from within residues 700 to the C-terminus of Mouse Gephyrin.

  Read Abcam's proprietary immunogen policy

  (Peptide available as ab32205.)

• Positive controlThis antibody gave a positive signal in the following whole cell lysates: Brain (Mouse) Brain (Mouse) - normal tissue, 0 days old NIH 3T3 (Mouse embryonic fibroblast cell line) MEF1 (Mouse embryonic fibroblast cell line) PC12 (Rat adrenal pheochromocytoma cell line) and the following tissue lysates: Liver (Mouse) Heart (Mouse) Kidney (Mouse) Pancreas (Human) Skeletal Muscle (Mouse) Liver (Rat) Heart (Rat) Kidney (Rat)

Properties

• FormLiquid
• Storage instructionsStore at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
• Storage bufferPreservative: 0.02% Sodium Azide
  Constituents: 1% BSA, PBS, pH 7.4
• Concentration information loading...
• PurityImmunogen affinity purified
• Clonality Polyclonal
• IsotypeIgG
• Research Areas
  • Neuroscience
  • Cell Adhesion Proteins
  • Cytoskeletal Proteins
  • Microtubules

  • Neuroscience
  • Cell Adhesion Proteins
  • Membrane Proteins

Applications

Target

• FunctionMicrotubule-associated protein involved in membrane protein-cytoskeleton interactions. It is thought to anchor the inhibitory glycine receptor (GLYR) to subsynaptic microtubules (By similarity). Catalyzes two steps in the biosynthesis of the molybdenum cofactor. In the first step, molybdopterin is adenylated. Subsequently, molybdate is inserted into adenylated molybdopterin and AMP is released.
• PathwayCofactor biosynthesis; molybdopterin biosynthesis.
• Involvement in diseaseDefects in GPHN are the cause of molybdenum cofactor deficiency type C (MOCOD type C) [MIM:252150]. MOCOD type C is an autosomal recessive disease which leads to the pleiotropic loss of all molybdoenzyme activities and is characterized by severe neurological damage, neonatal seizures and early childhood death.
  Defects in GPHN are a cause of startle disease (STHE) [MIM:149400]; also known as hyperekplexia. STHE is a genetically heterogeneous neurologic disorder characterized by muscular rigidity of central nervous system origin, particularly in the neonatal period, and by an exaggerated startle response to unexpected acoustic or tactile stimuli.
• Sequence similaritiesIn the N-terminal section; belongs to the moaB/mog family.
  In the C-terminal section; belongs to the moeA family.
• Cellular localizationCell junction > synapse. Cell junction > synapse > postsynaptic cell membrane. Cytoplasm > cytoskeleton. Cytoplasmic face of glycinergic postsynaptic membranes.

Target information above from: UniProt accession Q9NQX3 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 428878 Chicken
  • Entrez Gene: 10243 Human
  • Entrez Gene: 10243 Human
  • Entrez Gene: 268566 Mouse
  • Entrez Gene: 64845 Rat
  • Omim: 603930 Human
  • SwissProt: Q9NQX3 Human
  • SwissProt: Q9NQX3 Human

  • SwissProt: Q8BUV3 Mouse
  • SwissProt: Q03555 Rat
  • Unigene: 208765 Human
  • Unigene: 341742 Mouse
  • Unigene: 363753 Mouse
  • Unigene: 453131 Mouse
  • Unigene: 11032 Rat

  see all

• Alternative names
  Domain E antibodyDomain G antibodyGEPH antibody

  GEPH_HUMAN antibodyGPH antibodyGPHN antibodyGPHRYN antibodyKIAA1385 antibodyMolybdopterin molybdenumtransferase antibodyMPT adenylyltransferase antibodyMPT Mo-transferase antibody

  see all

Anti-Gephyrin antibody images

• 

  Western blot
  All lanes : Anti-Gephyrin antibody (ab32206) at 1 µg/ml
  
  Lane 1 : Brain (Mouse) Whole Cell Lysate - Adult
  Lane 2 : Brain (Mouse) Tissue Lysate - normal tissue, 0 days old (ab7188) at 20 µg
  Lane 3 : Brain (Mouse) Whole Cell Lysate - Adult at 20 µg with Gephyrin peptide (ab32205) at 1 µg/ml
  Lane 4 : Brain (Mouse) Tissue Lysate - normal tissue, 0 days old (ab7188) at 20 µg with Gephyrin peptide (ab32205) at 1 µg/ml
  
  Secondary
  Goat polyclonal to Rabbit IgG (Alexa Fluor® 680) at 1/10000 dilution
  
  Performed under reducing conditions.
  
  Predicted band size : 83 kDa
  Observed band size : 54,75,93 kDa (why is the actual band size different from the predicted?)
  
• 

  Immunohistochemistry - Free Floating - Gephyrin antibody (ab32206)Sophie Pezet, CNRS, Paris
  Immunofluorescent staining for Gephyrin in rat brain cortex using Rabbit polyclonal to Gephyrin (ab32206; 1/1000). The staining is located in the cytoplasm and in some of the processes of cortical neurons. This staining is observed in many other brain areas. The picture was acquired using a X20 objective. Protocol details: Rats were intracardially perfused with 4% paraformaldehyde. Whole brain tissue was post-fixed overnight in the same fixative, and cryoprotected in 20% sucrose and frozen in OCT. 30µm coronal sections were cut by cyrostat for use in fre floating IHC. Primary antibody ab32206 was incubated overnight at 1/1000 at room temperature. Secondary antibody Alexa fluor 488 1/1000 was incubated for 2 hours at room temperature.
• 

  Western blot - Gephyrin antibody (ab32206)
  All lanes : Anti-Gephyrin antibody (ab32206) at 1 µg/ml
  
  Lane 1 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate (ab7179)
  Lane 2 : MEF1 (Mouse embryonic fibroblast cell line) Whole Cell Lysate
  Lane 3 : Liver (Mouse) Tissue Lysate - normal tissue
  Lane 4 : Heart (Mouse) Tissue Lysate
  Lane 5 : Kidney (Mouse) Tissue Lysate
  Lane 6 : Pancreas (Mouse) Tissue Lysate (ab29363)
  Lane 7 : Skeletal Muscle (Mouse) Tissue Lysate (ab29711)
  Lane 8 : PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate
  Lane 9 : Liver (Rat) Tissue Lysate
  Lane 10 : Heart (Rat) Tissue Lysate
  Lane 11 : Kidney (Rat) Whole Cell Lysate - normal tissue (ab29480)
  
  Lysates/proteins at 10 µg per lane.
  
  Secondary
  IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
  
  Performed under reducing conditions.
  
  Predicted band size : 83 kDa
  Observed band size : 75,93 kDa (why is the actual band size different from the predicted?)
  
• 

  Immunocytochemistry/ Immunofluorescence - Gephyrin antibody (ab32206)
  ICC/IF image of ab32206 stained MEF1 cells. The cells were 4% PFA fixed (10 min), permabilised in 0.1% PBS-Tween (20 min) and incubated with the antibody (ab32206, 1µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).
• 

  Immunocytochemistry/ Immunofluorescence - Anti-Gephyrin antibody (ab32206)
  ICC/IF image of ab32206 stained PC12 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab32206, 1µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.