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Germinal Center Kinase protein (Active) (ab89753)

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Overview

  • Product nameGerminal Center Kinase protein (Active)See all Germinal Center Kinase proteins and peptides ...
  • Protein descriptionRecombinant full length Human Germinal Center Kinase with N-terminal proprietary tag expressed in a Baculovirus infected Sf9 cell expression system; amino acids 1-820, 125kDa. UniProt = Q12851.
  • Expression hostBaculovirus infected sf9 cells

    • Properties

  • Purification notesPurity was determined to be >80% by densitometry.
  • Biological activityThe Specific activity of ab89753 was determined to be 290 nmol/min/mg.
  • FormLiquid
  • Storage instructionsShipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.This product is an active protein and may elicit a biological response in vivo, handle with caution.
  • Storage bufferPreservative: None
    Constituents: 25% Glycerol, 50mM Tris HCl, 150mM Sodium chloride, 0.25mM DTT, 0.1mM EGTA, 0.1mM EDTA, 0.1mM PMSF, pH 7.5
  • Concentration information loading...
  • Research Areas

    • Applications

    Our Abpromise guarantee covers the use of ab89753 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    SDS-PAGE SDS-PAGE: Use at an assay dependent dilution.
    WB WB: Use at an assay dependent dilution.
    Functional Studies FuncS: Use at an assay dependent dilution.
  • Application notesThis peptide can be used with studies using ab167532 and ab170695.

    • Protein info

    • Alternative names
        B lymphocyte serine/threonine protein kinaseB lymphocyte serine/threonine-protein kinaseBL44
        GC kinaseGCKGerminal center kinasegerminal centre kinase (GC kinase)M4K2_HUMANMAP4K2MAPK/ERK kinase kinase kinase 2MEK kinase kinase 2MEKKK 2Mitogen activated protein kinase kinase kinase kinase 2Mitogen-activated protein kinase kinase kinase kinase 2Rab8 interacting proteinRab8 interacting protein, formerlyRab8-interacting proteinRAB8IPRAB8IP, formerly
      see all
  • FunctionEnhances MAP3K1 oligomerization, which may relieve amino-terminal mediated MAP3K1 autoinhibition and lead to activation following autophosphorylation. May play a role in the regulation of vesicle targeting or fusion.
  • Tissue specificityHighly expressed in germinal center but not mantle zone B-cells. Also expressed in lung, brain and placenta and at lower levels in other tissues examined.
  • Sequence similaritiesBelongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.
    Contains 1 CNH domain.
    Contains 1 protein kinase domain.
  • Cellular localizationCytoplasm. Basolateral cell membrane. Golgi apparatus membrane.

    • Target information above from: UniProt accession Q12851 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt

    Germinal Center Kinase protein (Active) images

    • SDS-PAGE - Germinal Center Kinase protein (Active) (ab89753)
      SDS-PAGE - Germinal Center Kinase protein (Active) (ab89753)
      SDS-PAGE showing ab89753 at approximately 125kDa.
    • Functional Studies - Germinal Center Kinase protein (Active) (ab89753)
      Functional Studies - Germinal Center Kinase protein (Active) (ab89753)
      Kinase Assay demonstrating specific activity of ab89753.

    References for Germinal Center Kinase protein (Active) (ab89753)

    ab89753 has not yet been referenced specifically in any publications.

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