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Overview

  • Product nameAnti-Ghrelin antibodySee all Ghrelin primary antibodies ...
  • Description
    Rabbit polyclonal to Ghrelin
  • SpecificityReacts specifically with human 13 kDa protein
  • Tested applicationsIHC-P, WB more details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide derived from Ghrelin protein

Properties

Applications

Our Abpromise guarantee covers the use of ab64325 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
IHC-P IHC-P: Use at an assay dependent dilution.
WB WB: 1/500 - 1/5000. Predicted molecular weight: 13 kDa.

Target

  • FunctionGhrelin is the ligand for growth hormone secretagogue receptor type 1 (GHSR). Induces the release of growth hormone from the pituitary. Has an appetite-stimulating effect, induces adiposity and stimulates gastric acid secretion. Involved in growth regulation.
    Obestatin may be the ligand for GPR39. May have an appetite-reducing effect resulting in decreased food intake. May reduce gastric emptying activity and jejunal motility.
  • Tissue specificityHighest level in stomach. All forms are found in serum as well. Other tissues compensate for the loss of ghrelin synthesis in the stomach following gastrectomy.
  • Sequence similaritiesBelongs to the motilin family.
  • Post-translational
    modifications    O-octanoylation or O-decanoylation is essential for ghrelin activity. The O-decanoylated forms Ghrelin-27-C10 and Ghrelin-28-C10 differ in the length of the carbon backbone of the carboxylic acid bound to Ser-26. A small fraction of ghrelin, ghrelin-28-C10:1, may be modified with a singly unsaturated carboxylic acid.
    Amidation of Leu-98 is essential for obestatin activity.
  • Cellular localizationSecreted.

    • Target information above from: UniProt accession Q9UBU3 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links
    • Alternative names
        Appetite regulating hormone antibodyGhrelin 27 antibodyGhrelin 28 antibody
        Ghrelin antibodyGhrelin/obestatin prepropeptide antibodyghrl antibodyGHRL_HUMAN antibodyGrowth hormone releasing peptide antibodyGrowth hormone secretagogue antibodyGrowth hormone-releasing peptide antibodyM46 protein antibodyMotilin related peptide antibodyMotilin-related peptide antibodyMTLRP antibodyObestatin antibodyProtein M46 antibody
      see all

    References for Anti-Ghrelin antibody (ab64325)

    This product has been referenced in:
    • Aghajanova L  et al. Diminished endometrial expression of ghrelin and ghrelin receptor contributes to infertility. Reprod Sci 17:823-32 (2010). Read more (PubMed: 20616368) »

    See 1 Publication for this product

    Publishing research using ab64325 ? Please let us know so that we can cite the reference in this datasheet

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    10
    Votes: Highest First

    Now I hope you can help me to understand if ab 64325 binds obestatin
    too. Probably ab 64325 isn't able to bind obestatin because the
    antibody is generated against a peptide derived from the mature form,
    as you elucidated, but now, ...

    Read More

    Thank you for your enquiry.

    The antibody is not capable of detecting obestatin, as it is raised against a peptide derived from mature ghrelin. It appears in a search using the search term "obestatin" because obestatin is a product of the GHRL g...

    Read More

    Kind scientific technical expert, I urgently need to know whether the
    antibody ab 64325, binds the prepro, pro and mature form of human
    ghrelin. Thanks for your help!!

    Thank you for contacting us.

    The antibody is generated against a peptide derived from the mature form. This sequence of amino acids is contained also in the precursor forms (prepro and pro), so in theory the antibody should be capable of recogn...

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"