Anti-Giantin antibody (ab24586)

  Immunocytochemistry/ Immunofluorescence - Giantin antibody - Golgi Marker (ab24586)

ab24586 at a 1/200 dilution staining mouse C2 myoblasts by ICC/IF. The cells were paraformaldehyde fixed and blocked with 3% BSA and incubated with antibody for 1 hour. Bound antibody was detected using an Alex Fluor ®488 conjugated donkey anti-rabbit IgG (H+L).

This image is courtesy of an Abreview submitted by Miss Anja Drozd

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  Immunocytochemistry/ Immunofluorescence - Giantin antibody - Golgi Marker (ab24586)

ab24586 at a 1/500 dilution staining HeLa cells (green) by Immunocytochemistry/Immunofluorescence. Other structures visualized with monoclonal anti-TU27 (red).

  Immunocytochemistry/ Immunofluorescence - Giantin antibody (ab24586)

ICC/IF image of ab24586 stained Hela cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab24586, 1µg/ml) overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti-rabbit IgG - H&L, pre-adsorbed (ab96899) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  Immunohistochemistry (Frozen sections) - Giantin antibody (ab24586)

ab24586 staining Giantin in E10.5 Mouse otic epithelium tissue by Immunohistochemistry (Frozen sections). The sections were PFA-fixed prior to blocking with 5% serum for 1 hour at 37°C. The primary antibody was diluted 1/500 and incubated with the sample for 1 hour at 37°C. An Alexa Fluor® 568-conjugated Goat anti-Rabbit IgG polyclonal was used as the secondary antibody, diluted 1/500.

This image is courtesy of an Anonymous Abreview.

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