Overview

  • Product name
    Giardia lamblia antigen ELISA Kit
  • Detection method
    Colorimetric
  • Precision
    Intra-assay
    Sample n Mean SD CV%
    1 12 6.86%
    2 12 6.41%
    3 12 6.36%
    4 12 5.04%
    Inter-assay
    Sample n Mean SD CV%
    1 11 4.77%
    2 11 4.64%
    3 11 7.77%
    4 11 10.9%
  • Sample type
    Other biological fluids
  • Assay type
    Indirect
  • Sensitivity
    = 97.5 %
  • Assay duration
    Multiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    Abcam’s Giardia lamblia in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate qualitative measurement of Giardia lamblia antigens in faeces.

     

    A 96-well plate has been precoated with polyclonal anti-Giardia lamblia antibodies. Controls or test samples are added to the wells and incubated; Giardia antigens present in the stool supernatant are captured. Following washing, a horseradish peroxidase (HRP) labelled anti-Giardia antibody is added to the wells, which binds to the immobilized antibodies. TMB is then catalyzed by the HRP to produce a blue color product that changes to yellow after adding an acidic stop solution. The density of yellow coloration is directly proportional to the amount of Giardia lamblia antigen sample captured in plate.

  • Tested applications
    Suitable for: Indirect ELISAmore details
  • Platform
    Microplate

Properties

  • Storage instructions
    Store at +4°C. Please refer to protocols.
  • Components 1 x 96 tests
    10X Wash Solution 1 x 100ml
    Anti-Giardia lamblia Coated Microplate (12 x 8 well strips) 1 unit
    Anti-Giardia lamblia Conjugate 1 x 12ml
    Cover Foil 1 unit
    Giardia lamblia Antigen Negative Control 1 x 2ml
    Giardia lamblia Antigen Positive Control 1 x 2ml
    Sample Diluent 1 x 100ml
    Stop Solution 1 x 15ml
    Strip holder 1 unit
    TMB Substrate Solution 1 x 15ml

Applications

Our Abpromise guarantee covers the use of ab178643 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Indirect ELISA Use at an assay dependent concentration.

Protocols

References

ab178643 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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