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ab86150 |
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ab111659 |
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Read our guarantee »Publishing research using ab49314? Please let us know so that we can cite the reference in this datasheet
ab49314 has been referenced in 5 publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Lane 1 : MARKER
Lane 2 : Anti-Gli1 antibody (ab49314) at 2.5 µg/ml
Lane 1 : As above
Lane 2 : HepG2 cell lysate at 10 µg
Secondary
Lane 1 :
Lane 2 : HRP conjugated anti-Rabbit IgG at 1/50000 dilution
Predicted band size : 118 kDa
Observed band size : 118 kDa
Lane 1 : Anti-Gli1 antibody (ab49314) at 1.25 µg/ml
Lane 2 : Anti-Gli1 antibody (ab49314) at 2.5 µg/ml
Lane 3 : Anti-Gli1 antibody (ab49314) at 5 µg/ml
Lane 1 : Cell lysate prepared from human HepG2 cells
Lane 2 : Cell lysate prepared from human HepG2 cells
Lane 3 : Cell lysate prepared from human HepG2 cells
Lysates/proteins at 25 µg per lane.
Predicted band size : 118 kDa
ICC/IF image of ab49314 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab49314, 5µg/ml) overnight at +4ºC. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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