Loading...
You have changed your country from
to
. Please be aware that this will change the currency in the purchasing process.
Products:Epigenetics and Nuclear Signaling >> Transcription >> Domain Families >> Zinc Finger
Overview
Product name
Anti-Gli1 antibody
See all Gli1 products (3) ...
Description
Rabbit polyclonal to Gli1
Specificity
This antibody is specific for Gli1.
Tested applications
ICC/IF, WB, ELISAmore details
Cross reactivity
Reacts with
Human
Predicted to work with
Mouse, Cow, Dog
Immunogen
A region within Synthetic peptide: TNPSCGHPEV GRLGGGPALY PPPEGQVCNP LDSLDLDNTQ LDFVAILDEP , corresponding to amino acids 1007-1056 of Human Gli1
TNPSCGHPEV GRLGGGPALY PPPEGQVCNP LDSLDLDNTQ LDFVAILDEP
Positive control
HepG2 cell lysate.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: 2% Sucrose, PBS
Concentration
Concentration information loading...
Purity
Protein A purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Epigenetics and Nuclear Signaling >> Transcription >> Domain Families >> Krueppel like factor
Cancer >> Oncoproteins/suppressors >> Tumor suppressors >> PTC & Wnt pathway
Stem Cells >> Signaling Pathways >> Hedgehog >> Nuclear
Neuroscience >> Neurology process >> Neural Signal Transduction
Epigenetics and Nuclear Signaling >> Transcription >> Domain Families >> Zinc Finger
Applications
Show applications key
Our Abpromise guarantee covers the use of ab49314 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ICC/IF: Use a concentration of 5 µg/ml
WB: Use a concentration of 2.5 µg/mlDetects a band of approximately 118 kDa (predicted molecular weight: 118 kDa).Can be blocked with Gli1 peptide (1009-1058) (ab86150). (5% skim milk in PBS buffer. (An HRP conjugated anti-Rabbit IgG could be used as a secondary, a suggested dilution of 1/50000 to 1/100000). )
ELISA
ELISA: 1/312500ELISA: 1/312500
Target
Relevance
Gli1 is a member of the Kruppel family of zinc finger proteins. The Gli transcription factors constitute the final effectors of the Hedgehog signalling pathway, which is critical to vertebrate development. Gli1 may play a role in craniofacial and digital development, as well as development of the central nervous system and gastrointestinal tract. Ectopic activation of Gli proteins is linked to tumorigenesis. In addition, both the Ras and Transforming Growth Factor Beta pathways also stimulate and/or induce Gli1 and Gli2 activity independently of Hedgehog ligands.
Cellular localization
Cytoplasmic and Nuclear
Alternative names
- Gli 1 antibody
- GLI antibody
- GLI Kruppel family member 1 antibody
see all
Database links
- Entrez Gene: 517588 Cow
- Entrez Gene: 2735 Human
- Entrez Gene: 14632 Mouse
- Omim: 165220 Human
- SwissProt: A6H727 Cow
- SwissProt: P08151 Human
- SwissProt: P47806 Mouse
- Unigene: 632702 Human
Anti-Gli1 antibody images:
Western blot - Gli1 antibody (ab49314)
Lane 1 : MARKER
Lane 2 : Anti-Gli1 antibody (ab49314) at 2.5 µg/ml
Lane 1 : As above
Lane 2 : HepG2 cell lysate at 10 µg
Secondary
Lane 1 :
Lane 2 : HRP conjugated anti-Rabbit IgG at 1/50000 dilution
Predicted band size : 118 kDa
Observed band size : 118 kDa
Western blot - Gli1 antibody (ab49314)
Lane 1 : Anti-Gli1 antibody (ab49314) at 1.25 µg/ml
Lane 2 : Anti-Gli1 antibody (ab49314) at 2.5 µg/ml
Lane 3 : Anti-Gli1 antibody (ab49314) at 5 µg/ml
Lane 1 : Cell lysate prepared from human HepG2 cells
Lane 2 : Cell lysate prepared from human HepG2 cells
Lane 3 : Cell lysate prepared from human HepG2 cells
Lysates/proteins at 25 µg per lane.
Predicted band size : 118 kDa
Immunocytochemistry/ Immunofluorescence-Gli1 antibody(ab49314)
ICC/IF image of ab49314 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab49314, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
References for Anti-Gli1 antibody (ab49314)
This product has been referenced in:
- Yoshimura Ket al. Signaling through the primary cilium affects glial cell survival under a stressed environment. Glia 59:333-44 (2011). WB; Rat.Read more (PubMed: 21125655) »
- Maitah MYet al. Up-regulation of sonic hedgehog contributes to TGF-ß1-induced epithelial to mesenchymal transition in NSCLC cells. PLoS One 6:e16068 (2011). WB; Human.Read more (PubMed: 21249152) »
See all 4 publications for this product
Publishing research using ab49314? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"