Overview
- Product nameAnti-Gli1 antibodySee all Gli1 primary antibodies ...
- DescriptionRabbit polyclonal to Gli1
- SpecificityThis antibody is specific for Gli1.
- Tested applicationsICC/IF, WB, ELISA more details
- Species reactivityReacts with: Mouse, Human
Predicted to work with: Cow, Dog - Immunogen
A region within Synthetic peptide: TNPSCGHPEV GRLGGGPALY PPPEGQVCNP LDSLDLDNTQ LDFVAILDEP , corresponding to amino acids 1007-1056 of Human Gli1
- Positive controlHepG2 cell lysate.
Properties
- FormLiquid
- Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
- Storage bufferPreservative: None
Constituents: 2% Sucrose, PBS -
Concentration information loading... - PurityProtein A purified
- Clonality Polyclonal
- IsotypeIgG
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Research Areas
Applications
Our Abpromise guarantee covers the use of ab49314 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Notes |
|---|---|
| ICC/IF | ICC/IF: Use a concentration of 5 µg/ml. |
| WB | WB: Use a concentration of 2.5 µg/ml. Detects a band of approximately 118 kDa (predicted molecular weight: 118 kDa).Can be blocked with Gli1 peptide (ab111659) or Gli1 peptide (1009-1058) (ab86150). 5% skim milk in PBS buffer. (An HRP conjugated anti-Rabbit IgG could be used as a secondary, a suggested dilution of 1/50000 to 1/100000). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T. |
| ELISA | ELISA: 1/312500. |
Target
- FunctionActs as a transcriptional activator. May regulate the transcription of specific genes during normal development. May play a role in craniofacial development and digital development, as well as development of the central nervous system and gastrointestinal tract. Mediates SHH signaling and thus cell proliferation and differentiation.
- Tissue specificityTestis, myometrium and fallopian tube. Also expressed in the brain with highest expression in the cerebellum, optic nerve and olfactory tract.
- Sequence similaritiesBelongs to the GLI C2H2-type zinc-finger protein family.
Contains 5 C2H2-type zinc fingers. - Post-translational
modificationsPhosphorylated in vitro by ULK3. - Cellular localizationCytoplasm. Nucleus. Tethered in the cytoplasm by binding to SUFU. Activation and translocation to the nucleus is promoted by interaction with STK36. Phosphorylation by ULK3 may promote nuclear localization. Translocation to the nucleus is promoted by interaction with ZIC1.
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Database links
- Entrez Gene: 517588 Cow
- Entrez Gene: 2735 Human
- Entrez Gene: 14632 Mouse
- Omim: 165220 Human
- SwissProt: P08151 Human
- SwissProt: P47806 Mouse
- Unigene: 632702 Human
- Unigene: 391450 Mouse
Target information above from: UniProt accession
P08151
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010)
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Alternative names
- Gli 1 antibodyGLI antibodyGLI family zinc finger 1 antibody
- GLI Kruppel family member 1 antibodygli1 antibodyGLI1_HUMAN antibodyGlioma associated oncogene 1 antibodyGlioma associated oncogene antibodyGlioma associated oncogene homolog 1 (zinc finger protein) antibodyGlioma associated oncogene homolog antibodyGlioma-associated oncogene antibodyOncogene GLI antibodyZfp 5 antibodyZfp5 antibodyZinc finger protein GLI 1 antibodyZinc finger protein GLI1 antibody
see all
Anti-Gli1 antibody images
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Western blot - Gli1 antibody (ab49314)Lane 1 : MARKER
Lane 2 : Anti-Gli1 antibody (ab49314) at 2.5 µg/ml
Lane 1 : As above
Lane 2 : HepG2 cell lysate at 10 µg
Secondary
Lane 1 :
Lane 2 : HRP conjugated anti-Rabbit IgG at 1/50000 dilution
Predicted band size : 118 kDa
Observed band size : 118 kDa -
Western blot - Gli1 antibody (ab49314)Lane 1 : Anti-Gli1 antibody (ab49314) at 1.25 µg/ml
Lane 2 : Anti-Gli1 antibody (ab49314) at 2.5 µg/ml
Lane 3 : Anti-Gli1 antibody (ab49314) at 5 µg/ml
Lane 1 : Cell lysate prepared from human HepG2 cells
Lane 2 : Cell lysate prepared from human HepG2 cells
Lane 3 : Cell lysate prepared from human HepG2 cells
Lysates/proteins at 25 µg per lane.
Predicted band size : 118 kDa -
Immunocytochemistry/ Immunofluorescence-Gli1 antibody(ab49314)ICC/IF image of ab49314 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab49314, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
References for Anti-Gli1 antibody (ab49314)
This product has been referenced in:
- Ortega MC et al. Megalin mediates the influence of sonic hedgehog on oligodendrocyte precursor cell migration and proliferation during development. Glia 60:851-66 (2012). Read more (PubMed: 22354480) »
- Pandolfi S et al. WIP1 phosphatase modulates the Hedgehog signaling by enhancing GLI1 function. Oncogene : (2012). Read more (PubMed: 23146903) »
