Gli1 antibody (ab49314)

Product Name 

Gli1 antibody

Product type 

Primary antibodies

Description 

Rabbit polyclonal to Gli1

Immunogen 

Synthetic peptide: PSCGHPEVGR LGGGPALYPP PEGQVCNPLD SLDLDNTQLD FVAILDEPQG, corresponding to amino acids 1009-1058 of Human Gli1
BLAST 'PSCGHPEVGR LGGGPALYPP PEGQVCNPLD SLDLDNTQLD FVAILDEPQG ' with or

Reacts with 
(species key)

Hu

Specificity 

This antibody is specific for Gli1.

Tested applications 
(see key)

ELISA, WB

Abreviews 

Customer reviews (see key)

Read about Abreviews (Read all Abreviews)

Application notes 
(see key)

Recommended dilutions
ELISA: Use at an assay dependent dilution. (Titre: 1/312500).
WB: Use at a concentration of 2.5 µg/ml in 5% skim milk in PBS buffer. (An HRP conjugated anti-Rabbit IgG could be used as a secondary, a suggested dilution of 1/50000 to 1/100000). Detects a band of approximately 118 kDa (predicted molecular weight: 118 kDa).

Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Positive control 
(see definition)

HepG2 cell lysate.

Cellular localization 

Cytoplasmic and Nuclear

Research areas 

Chromatin and Nuclear Signaling >> Transcription >> Domain Families >> Krueppel like factor
Cancer >> Oncoproteins/suppressors >> Tumor suppressors >> PTC & Wnt pathway
Stem Cells >> Signaling Pathways >> Hedgehog >> Nuclear
Neuroscience >> Neurology process >> Neural Signal Transduction
Chromatin and Nuclear Signaling >> Transcription >> Domain Families >> Zinc Finger

Relevance 

Gli1 is a member of the Kruppel family of zinc finger proteins. The Gli transcription factors constitute the final effectors of the Hedgehog signalling pathway, which is critical to vertebrate development. Gli1 may play a role in craniofacial and digital development, as well as development of the central nervous system and gastrointestinal tract. Ectopic activation of Gli proteins is linked to tumorigenesis. In addition, both the Ras and Transforming Growth Factor Beta pathways also stimulate and/or induce Gli1 and Gli2 activity independently of Hedgehog ligands.

Database links 

Raised in 

Rabbit

Clonality 

Polyclonal

Isotype 

IgG

Purity 

Protein A purified

Storage buffer 

Preservative: None
Constituents: 2% Sucrose, PBS

Form 

Liquid

Concentration 

1.000 mg/ml

Storage instructions 

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

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References for Gli1 antibody (ab49314)

  This product has been used in:

Rahnama F et al. Inhibition of GLI1 gene activation by Patched1. Biochem J 394:19-26 (2006). PubMed: 16229683

If you publish research using ab49314 please let us know so that we can cite the reference on this datasheet.

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