Overview

  • Product nameAnti-Gli1 antibodySee all Gli1 primary antibodies ...
  • Description
    Rabbit polyclonal to Gli1
  • SpecificityThis antibody is specific for Gli1.
  • Tested applicationsICC/IF, WB, ELISAmore details
  • Species reactivity
    Reacts with: Mouse, Dog, Human
    Predicted to work with: Rat, Rabbit, Horse, Guinea pig, Cow, Cat
  • Immunogen

    A region within Synthetic peptide: TNPSCGHPEV GRLGGGPALY PPPEGQVCNP LDSLDLDNTQ LDFVAILDEP , corresponding to amino acids 1007-1056 of Human Gli1

  • Positive control
    • HepG2 cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab49314 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 5 µg/ml.
WB Use a concentration of 2.5 µg/ml. Detects a band of approximately 118 kDa (predicted molecular weight: 118 kDa).Can be blocked with Human Gli1 peptide (ab111659) or Human Gli1 peptide (ab86150). 5% skim milk in PBS buffer. (An HRP conjugated anti-Rabbit IgG could be used as a secondary, a suggested dilution of 1/50000 to 1/100000). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA 1/312500.

Target

  • FunctionActs as a transcriptional activator. May regulate the transcription of specific genes during normal development. May play a role in craniofacial development and digital development, as well as development of the central nervous system and gastrointestinal tract. Mediates SHH signaling and thus cell proliferation and differentiation.
  • Tissue specificityTestis, myometrium and fallopian tube. Also expressed in the brain with highest expression in the cerebellum, optic nerve and olfactory tract.
  • Sequence similaritiesBelongs to the GLI C2H2-type zinc-finger protein family.
    Contains 5 C2H2-type zinc fingers.
  • Post-translational
    modifications
    Phosphorylated in vitro by ULK3.
  • Cellular localizationCytoplasm. Nucleus. Tethered in the cytoplasm by binding to SUFU. Activation and translocation to the nucleus is promoted by interaction with STK36. Phosphorylation by ULK3 may promote nuclear localization. Translocation to the nucleus is promoted by interaction with ZIC1.
  • Information by UniProt
  • Database links
  • Alternative names
    • Gli 1 antibody
    • GLI antibody
    • GLI family zinc finger 1 antibody
    • GLI Kruppel family member 1 antibody
    • gli1 antibody
    • GLI1_HUMAN antibody
    • Glioma associated oncogene 1 antibody
    • Glioma associated oncogene antibody
    • Glioma associated oncogene homolog 1 (zinc finger protein) antibody
    • Glioma associated oncogene homolog antibody
    • Glioma-associated oncogene antibody
    • Oncogene GLI antibody
    • Zfp 5 antibody
    • Zfp5 antibody
    • Zinc finger protein GLI 1 antibody
    • Zinc finger protein GLI1 antibody
    see all

Anti-Gli1 antibody images


  • developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 118 kDa
    Observed band size : 118 kDa


    Exposure time : 20 minutes

    SKNSH cells were incubated at 37°C for 30 minutes with vehicle control (0 μM) and different concentrations of VU0155041 sodium salt (ab120248). Increased expression of Gli1 (ab49314) in SKNSH cells correlates with an decrease in VU0155041 sodium salt concentration, as described in literature.

    Whole cell lysates were prepared with RIPA buffer (containing protease inhibitors and sodium orthovanadate), 30 μg of each were loaded on the gel and the WB was run under reducing conditions. After transfer the membrane was blocked for an hour using 5% BSA before being incubated with ab49314 at 2.5 μg/ml and ab8227 at 1 μg/ml overnight at 4°C. Antibody binding was detected using an anti- rabbit antibody conjugated to HRP (ab97051) at 1/10000 and visualised using ECL development solution.

  • Lane 1 : MARKER
    Lane 2 : Anti-Gli1 antibody (ab49314) at 2.5 µg/ml

    Lane 1 : As above
    Lane 2 : HepG2 cell lysate at 10 µg

    Secondary
    Lane 1 :
    Lane 2 : HRP conjugated anti-Rabbit IgG at 1/50000 dilution


    Predicted band size : 118 kDa
    Observed band size : 118 kDa
  • Lane 1 : Anti-Gli1 antibody (ab49314) at 1.25 µg/ml
    Lane 2 : Anti-Gli1 antibody (ab49314) at 2.5 µg/ml
    Lane 3 : Anti-Gli1 antibody (ab49314) at 5 µg/ml

    Lane 1 : Cell lysate prepared from human HepG2 cells
    Lane 2 : Cell lysate prepared from human HepG2 cells
    Lane 3 : Cell lysate prepared from human HepG2 cells

    Lysates/proteins at 25 µg per lane.


    Predicted band size : 118 kDa
  • ICC/IF image of ab49314 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab49314, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References for Anti-Gli1 antibody (ab49314)

This product has been referenced in:
  • Santini R  et al. SOX2 regulates self-renewal and tumorigenicity of human melanoma-initiating cells. Oncogene 0:N/A (2014). Human . Read more (PubMed: 24681955) »
  • Shahi MH  et al. Blocking Signaling at the Level of GLI Regulates Downstream Gene Expression and Inhibits Proliferation of Canine Osteosarcoma Cells. PLoS One 9:e96593 (2014). WB ; Dog . Read more (PubMed: 24810746) »

See all 10 Publications for this product

Product Wall

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step Hydrogen Peroxide as blocking agent for 10 minute(s) · Concentration: 3% · Temperature: 25°C
Antigen retrieval step Heat mediated
Sample Mouse Tissue sections (Uterus)
Specification Uterus
Permeabilization No
Fixative Paraformaldehyde
Username

Hugo

Verified customer

Submitted Jul 18 2014

Abreviews
Application Western blot
Loading amount 30 µg
Gel Running Conditions Reduced Denaturing (10% SDS PAGE)
Sample Mouse Tissue lysate - whole (Brain)
Specification Brain
Blocking step Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Jun 09 2014

Abreviews
Application Western blot
Loading amount 3 µg
Gel Running Conditions Reduced Denaturing (4-15% gradient)
Sample Rabbit Tissue lysate - whole (brain)
Specification brain
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Oct 29 2013

Application Immunocytochemistry/ Immunofluorescence
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
Sample Human Cell (MKN45)
Specification MKN45
Permeabilization Yes - Triton 100X
Fixative Formaldehyde
Username

Abcam user community

Verified customer

Submitted Jul 05 2013

Application Western blot
Specification AGS
Sample Human Cell lysate - whole cell (AGS)
Loading amount 30 µg
Gel Running Conditions Reduced Denaturing
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
Username

Ms. Dayeon Yu

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Submitted May 14 2013

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I am very pleased to hear you would like to accept our offer and test ab49314 on rabbit samples in western blotting. To redeem this offer, please submit an Abreview for rabbit and include this code in the “Additional Comme...

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Application Immunocytochemistry/ Immunofluorescence
Sample Mouse Cell (Intestine)
Specification Intestine
Fixative Paraformaldehyde
Permeabilization Yes - .05% tween
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: RT°C
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Verified customer

Submitted Sep 19 2012

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (limb sections)
Specification limb sections
Fixative Paraformaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: sodium citrate
Permeabilization Yes - 0.05% tween
Blocking step 5% goat serum 1% BSA in PBT as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 6% · Temperature: RT°C
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Mrs. Neta Maayan

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Submitted Jul 18 2012

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I am very pleased to hear you would like to accept our offer and test ab49314 in chicken samples. This code will give you: 1 free PRIMARY ANTIBODY before the expiration date. To redeem this offer, please...

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