Overview

  • Product nameAnti-Gli1 antibody
    See all Gli1 primary antibodies
  • Description
    Rabbit polyclonal to Gli1
  • Tested applicationsIHC-P, WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Human, Rhesus monkey
    Predicted to work with: Horse, Dog
  • Immunogen

    Recombinant fragment corresponding to a region within the C terminal amino acids 940-990 (RAKAPVNTYG PGFGPNLPNH KSGSYPTPSP CHENFVVGAN RASHRAAAPP R) of Human Gli1 (UniProt ID: P08151).

  • Positive control
    • Human brain tissue

Properties

Applications

Our Abpromise guarantee covers the use of ab92611 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB Use a concentration of 1 µg/ml.
ICC/IF Use a concentration of 5 µg/ml.

Target

  • FunctionActs as a transcriptional activator. May regulate the transcription of specific genes during normal development. May play a role in craniofacial development and digital development, as well as development of the central nervous system and gastrointestinal tract. Mediates SHH signaling and thus cell proliferation and differentiation.
  • Tissue specificityTestis, myometrium and fallopian tube. Also expressed in the brain with highest expression in the cerebellum, optic nerve and olfactory tract.
  • Sequence similaritiesBelongs to the GLI C2H2-type zinc-finger protein family.
    Contains 5 C2H2-type zinc fingers.
  • Post-translational
    modifications
    Phosphorylated in vitro by ULK3.
  • Cellular localizationCytoplasm. Nucleus. Tethered in the cytoplasm by binding to SUFU. Activation and translocation to the nucleus is promoted by interaction with STK36. Phosphorylation by ULK3 may promote nuclear localization. Translocation to the nucleus is promoted by interaction with ZIC1.
  • Information by UniProt
  • Database links
  • Alternative names
    • Gli 1 antibody
    • GLI antibody
    • GLI family zinc finger 1 antibody
    • GLI Kruppel family member 1 antibody
    • gli1 antibody
    • GLI1_HUMAN antibody
    • Glioma associated oncogene 1 antibody
    • Glioma associated oncogene homolog 1 (zinc finger protein) antibody
    • Glioma associated oncogene homolog antibody
    • Glioma-associated oncogene antibody
    • Oncogene GLI antibody
    • Zfp 5 antibody
    • Zfp5 antibody
    • Zinc finger protein GLI 1 antibody
    • Zinc finger protein GLI1 antibody
    see all

Anti-Gli1 antibody images

  • Immunohistochemical analysis of Gli1 in formalin fixed, paraffin embedded Human brain tissue using an isotype control (1) and ab92611 (2) at 5 µg/ml.
  • Lane 1 : Preimmune sera at 1/5000 dilution
    Lane 2 : Anti-Gli1 antibody (ab92611) at 1 µg/ml

    Lane 1 : Human kidney lysate
    Lane 2 : Human kidney lysate

    Secondary
    HRP conjugated goat anti rabbit IgG
    developed using the ECL technique
  • ICC/IF image of ab92611 stained SKNSH cells. The cells were 4% formaldehyde (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab92611, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References for Anti-Gli1 antibody (ab92611)

This product has been referenced in:
  • Machado D  et al. Irradiated riboflavin diminishes the aggressiveness of melanoma in vitro and in vivo. PLoS One 8:e54269 (2013). WB ; Mouse . Read more (PubMed: 23342114) »
  • Im S  et al. Hedgehog related protein expression in breast cancer: gli-2 is associated with poor overall survival. Korean J Pathol 47:116-23 (2013). IHC-P ; Human . Read more (PubMed: 23667370) »

See all 4 Publications for this product

Product Wall

Application Immunohistochemistry (Frozen sections)
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 25°C
Sample Mouse Tissue sections (Normal skin and skin tumors)
Specification Normal skin and skin tumors
Permeabilization No
Fixative Formaldehyde
Username

Abcam user community

Verified customer

Submitted Mar 28 2014

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (colon)
Specification colon
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Bond Epitope Retrieval 2
Permeabilization No
Username

Abcam user community

Verified customer

Submitted Apr 10 2013

The antibody is working in the positive controls but having low expression in your samples. There could be number of reason why lower signal is found in sample tissues. It may be that the expression levels of protein in this sample type are lower than ...

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DISCOUNT CODE: ABTRIAL-EBAMF
Expiration date: 17 March 2013
Value: €370.00

I am very pleased to hear you would like to use ab92611 in IHC-P on Mouse and send us an image of your results.

This code will give you a €3...

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Thank you very much for your reply.

The recommended starting dilutions in IHC-P are:

Ab32536: a 1:250 dilution
Ab31481: a 1:50 dilution
Ab134906: within the range of 1:250-1:500 dilution

A ChIP grade antibody has...

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Thank you very much for your email, and I'm sorry I didn't get to this earlier.

The package was delivered at 10 am this morning. The FedEx tracking number is ###, in case you need any more information.

Please let me know if you have...

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Thank you very much for your call today and for letting us know about the trouble with these antibodies.

As we discussed, I'm sending free of charge vials of ab32536, ab31481, and ab134906. These will arrive on Monday on the order ***.
<...

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Thank you for contacting us. I've listed the recommended running conditions below.

For ab7970: IHC-P: 1/1000 - 1/4000. Perform heat mediated antigen retrieval via the microwave method before commencing with IHC staining protocol.

F...

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Thank you for contacting us.

Abcam takes product quality seriously and we recently undertook an in-depth study examining storage conditions and antibody performance. After over 13,000 individual ELISA experiments, we have determined that sto...

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Vielen Dank für Ihre Anfrage. In der Tat konnte ich in unserer Datenbank nichts darüber finden, dass die genannten Antikörper nicht auch für die Durchflußzytometrie geeignet sein könnten. Ich habe Ihnen daher die entsprechenden Gutscheincodes ausgestel...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"