Anti-Gli1 antibody (ab92611)

Overview

• Product nameAnti-Gli1 antibodySee all Gli1 primary antibodies ...
• Description
  Rabbit polyclonal to Gli1
• Tested applicationsIHC-P, WB, ICC/IF more details
• Species reactivity
  Reacts with: Mouse, Human, Rhesus monkey
  Predicted to work with: Horse, Dog
• Immunogen

  Recombinant fragment corresponding to a region within the C terminal amino acids 940-990 (RAKAPVNTYG PGFGPNLPNH KSGSYPTPSP CHENFVVGAN RASHRAAAPP R) of Human Gli1 (UniProt ID: P08151).

  Run BLAST with Run BLAST with
• Positive controlHuman brain tissue

Properties

• FormLiquid
• Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
• Storage bufferPreservative: 0.05% Sodium Azide
  Constituents: 0.05% BSA, PBS
• Concentration information loading...
• PurityProtein A purified
• Clonality Polyclonal
• IsotypeIgG
• Research Areas
  • Stem Cells
  • Signaling Pathways
  • Hedgehog
  • Nuclear

  • Cancer
  • Oncoproteins/suppressors
  • Tumor suppressors
  • PTC & Wnt pathway

  • Epigenetics and Nuclear Signaling
  • Transcription
  • Domain Families
  • Krueppel like factor

  • Epigenetics and Nuclear Signaling
  • Transcription
  • Domain Families
  • Zinc Finger

  • Neuroscience
  • Neurology process
  • Neural Signal Transduction

Applications

Target

• FunctionActs as a transcriptional activator. May regulate the transcription of specific genes during normal development. May play a role in craniofacial development and digital development, as well as development of the central nervous system and gastrointestinal tract. Mediates SHH signaling and thus cell proliferation and differentiation.
• Tissue specificityTestis, myometrium and fallopian tube. Also expressed in the brain with highest expression in the cerebellum, optic nerve and olfactory tract.
• Sequence similaritiesBelongs to the GLI C2H2-type zinc-finger protein family.
  Contains 5 C2H2-type zinc fingers.
• Post-translational
  modificationsPhosphorylated in vitro by ULK3.
• Cellular localizationCytoplasm. Nucleus. Tethered in the cytoplasm by binding to SUFU. Activation and translocation to the nucleus is promoted by interaction with STK36. Phosphorylation by ULK3 may promote nuclear localization. Translocation to the nucleus is promoted by interaction with ZIC1.

Target information above from: UniProt accession P08151 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 100033937 Horse
  • Entrez Gene: 2735 Human
  • Entrez Gene: 14632 Mouse
  • Omim: 165220 Human
  • SwissProt: P08151 Human
  • SwissProt: P47806 Mouse
  • Unigene: 632702 Human
  • Unigene: 391450 Mouse

• Alternative names
  Gli 1 antibodyGLI antibodyGLI family zinc finger 1 antibody

  GLI Kruppel family member 1 antibodygli1 antibodyGLI1_HUMAN antibodyGlioma associated oncogene 1 antibodyGlioma associated oncogene homolog 1 (zinc finger protein) antibodyGlioma associated oncogene homolog antibodyGlioma-associated oncogene antibodyOncogene GLI antibodyZfp 5 antibodyZfp5 antibodyZinc finger protein GLI 1 antibodyZinc finger protein GLI1 antibody

  see all

Anti-Gli1 antibody images

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Gli1 antibody (ab92611)
  Immunohistochemical analysis of Gli1 in formalin fixed, paraffin embedded Human brain tissue using an isotype control (1) and ab92611 (2) at 5 µg/ml.
• 

  Western blot - Gli1 antibody (ab92611)
  Lane 1 : Preimmune sera at 1/5000 dilution
  Lane 2 : Anti-Gli1 antibody (ab92611) at 1 µg/ml
  
  Lane 1 : Human kidney lysate
  Lane 2 : Human kidney lysate
  
  Secondary
  HRP conjugated goat anti rabbit IgG
  developed using the ECL technique
• 

  Immunocytochemistry/ Immunofluorescence - Anti-Gli1 antibody (ab92611)
  ICC/IF image of ab92611 stained SKNSH cells. The cells were 4% formaldehyde (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab92611, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.