Anti-Gli1 antibody (ab92611)
- Product nameAnti-Gli1 antibodySee all Gli1 primary antibodies ...
- DescriptionRabbit polyclonal to Gli1
- Tested applicationsIHC-P, WB, ICC/IF more details
- Species reactivityReacts with: Mouse, Human, Rhesus monkey
Predicted to work with: Horse, Dog
Recombinant fragment corresponding to a region within the C terminal amino acids 940-990 (RAKAPVNTYG PGFGPNLPNH KSGSYPTPSP CHENFVVGAN RASHRAAAPP R) of Human Gli1 (UniProt ID: P08151).
- Positive controlHuman brain tissue
- Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
- Storage bufferPreservative: 0.05% Sodium Azide
Constituents: 0.05% BSA, PBS
- Concentration information loading...
- PurityProtein A purified
- Clonality Polyclonal
Our Abpromise guarantee covers the use of ab92611 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||IHC-P: Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
|WB||WB: Use a concentration of 1 µg/ml.|
|ICC/IF||ICC/IF: Use a concentration of 5 µg/ml.|
- FunctionActs as a transcriptional activator. May regulate the transcription of specific genes during normal development. May play a role in craniofacial development and digital development, as well as development of the central nervous system and gastrointestinal tract. Mediates SHH signaling and thus cell proliferation and differentiation.
- Tissue specificityTestis, myometrium and fallopian tube. Also expressed in the brain with highest expression in the cerebellum, optic nerve and olfactory tract.
- Sequence similaritiesBelongs to the GLI C2H2-type zinc-finger protein family.
Contains 5 C2H2-type zinc fingers.
modificationsPhosphorylated in vitro by ULK3.
- Cellular localizationCytoplasm. Nucleus. Tethered in the cytoplasm by binding to SUFU. Activation and translocation to the nucleus is promoted by interaction with STK36. Phosphorylation by ULK3 may promote nuclear localization. Translocation to the nucleus is promoted by interaction with ZIC1.
- Gli 1 antibodyGLI antibodyGLI family zinc finger 1 antibody
- GLI Kruppel family member 1 antibodygli1 antibodyGLI1_HUMAN antibodyGlioma associated oncogene 1 antibodyGlioma associated oncogene homolog 1 (zinc finger protein) antibodyGlioma associated oncogene homolog antibodyGlioma-associated oncogene antibodyOncogene GLI antibodyZfp 5 antibodyZfp5 antibodyZinc finger protein GLI 1 antibodyZinc finger protein GLI1 antibody
Anti-Gli1 antibody images
Immunohistochemical analysis of Gli1 in formalin fixed, paraffin embedded Human brain tissue using an isotype control (1) and ab92611 (2) at 5 µg/ml.
Lane 1 : Preimmune sera at 1/5000 dilution
Lane 2 : Anti-Gli1 antibody (ab92611) at 1 µg/ml
Lane 1 : Human kidney lysate
Lane 2 : Human kidney lysate
HRP conjugated goat anti rabbit IgG
developed using the ECL technique
ICC/IF image of ab92611 stained SKNSH cells. The cells were 4% formaldehyde (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab92611, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
References for Anti-Gli1 antibody (ab92611)
This product has been referenced in:
- Machado D et al. Irradiated riboflavin diminishes the aggressiveness of melanoma in vitro and in vivo. PLoS One 8:e54269 (2013). WB ; Mouse . Read more (PubMed: 23342114) »
- Ding H et al. Sonic hedgehog signaling mediates epithelial-mesenchymal communication and promotes renal fibrosis. J Am Soc Nephrol 23:801-13 (2012). WB ; Mouse . Read more (PubMed: 22302193) »