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Overview

  • Product nameAnti-Gli1 antibodySee all Gli1 primary antibodies ...
  • Description
    Rabbit polyclonal to Gli1
  • Tested applicationsWB more details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide from Human Gli1

  • Positive controlRMS-13 lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab94927 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
WB WB: Use a concentration of 10 µg/ml. Predicted molecular weight: 118 kDa. Note: 1/400 is the suggested starting working dilution.

Target

  • FunctionActs as a transcriptional activator. May regulate the transcription of specific genes during normal development. May play a role in craniofacial development and digital development, as well as development of the central nervous system and gastrointestinal tract. Mediates SHH signaling and thus cell proliferation and differentiation.
  • Tissue specificityTestis, myometrium and fallopian tube. Also expressed in the brain with highest expression in the cerebellum, optic nerve and olfactory tract.
  • Sequence similaritiesBelongs to the GLI C2H2-type zinc-finger protein family.
    Contains 5 C2H2-type zinc fingers.
  • Post-translational
    modifications    Phosphorylated in vitro by ULK3.
  • Cellular localizationCytoplasm. Nucleus. Tethered in the cytoplasm by binding to SUFU. Activation and translocation to the nucleus is promoted by interaction with STK36. Phosphorylation by ULK3 may promote nuclear localization. Translocation to the nucleus is promoted by interaction with ZIC1.

    • Target information above from: UniProt accession P08151 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links
    • Alternative names
        Gli 1 antibodyGLI antibodyGLI family zinc finger 1 antibody
        GLI Kruppel family member 1 antibodygli1 antibodyGLI1_HUMAN antibodyGlioma associated oncogene 1 antibodyGlioma associated oncogene homolog 1 (zinc finger protein) antibodyGlioma associated oncogene homolog antibodyGlioma-associated oncogene antibodyOncogene GLI antibodyZfp 5 antibodyZfp5 antibodyZinc finger protein GLI 1 antibodyZinc finger protein GLI1 antibody
      see all

    Anti-Gli1 antibody images

    • Western blot - Gli1 antibody (ab94927)
      Western blot - Gli1 antibody (ab94927)
      Anti-Gli1 antibody (ab94927) at 10 µg/ml + RMS-13 lysate at 15 µg

      Secondary
      Mouse anti-rabbit HRP at 1/200000 dilution

      Predicted band size : 118 kDa

    References for Anti-Gli1 antibody (ab94927)

    ab94927 has not yet been referenced specifically in any publications.

    Publishing research using ab94927 ? Please let us know so that we can cite the reference in this datasheet

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    Hi! Karen, Thanks for the reply in Sep 2011. We tried the optimize the WB according to your suggestion. Unfortunately, it didn't work for this antibody. Do you have any idea or should we try other antibody? Attached is the details of repeated exp...

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    Thank you for your reply. I am sorry to hear the results have not improved. The only other thing that might help is incubating the primary antibody at room temperature for 4-6 hours. However, if there is no improvement I can replace with the same product ...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"