Products:Epigenetics and Nuclear Signaling >> Transcription >> Domain Families >> Zinc Finger
|
ab111657 |
|
|
ab130900 |
If your product does not perform as described on this datasheet, we will refund or replace your product...
Read our guarantee »This product is covered by the Abpromise guarantee. Our scientific support team are available to answer any questions or queries - fill out an inquiry form for ab26056 for help.
Alternatively, you can search the previous enquiries about this product to see if your query has already been answered.
|
|||||||||
|
|||||||||
Thank you for your reply. is it possible to order the blocking peptide for this antibody? |
|||||||||
ANSWER: |
Thank you for contacting us. |
||||||||
|
|||||||||
I was studying a lot Gli2 and was trying to choose Gli2 antibody which would be correct to use to detect active form of Gli2 . As you may know human Gli2 has N-terminus region (repressor), DNA binding Zn finger region [437-589 aa] (this info is from Ensemble and from UniProtKB) and C-terminus region (activator) [590-1586]. Despite of the fact that Gli2 is presented in 5 isoforms, DNA biding region is conservative 1586 aa – 168 kDa (full length) 1258 aa – 133 kDa (1-328: Missing) 1241 aa – 132 kDa (1-328; Missing; 394-410: Missing) 829 aa – 88 kDa (1-328: Missing; 1149-1157: VSSGTVDAL → ASATWLSGT; 1158-1586: Missing) 812 aa – 86.5 kDa (1-328: Missing; 394-410: Missing; 1149-1157: VSSGTVDAL → ASATWLSGT; 1158-1586: Missing) In addition, post-translational modifications (such as acetylation which is important for Gli2 activity etc.) and mutations might also change presented above Gli2 MW in different cancers and/or cell types. Your anti-Gli2 antibody (ab26056) is ChIP grade. How it is possible if this antibody was generated using as immunogen a synthetic peptide derived from a region between residues 272 - 321 (which is within N-terminus, and for Gli2 activation, this region should be degraded)? |
|||||||||
ANSWER: |
Thank you for contacting us and for bringing this to our attention. |
||||||||
|
|||||||||
Is it possible to purchase a bloking peptide for Gli2 antibody (cat # ab26056) ? |
|||||||||
ANSWER: |
Thank you very much for contacting us. |
||||||||
|
|||||||||
vielen dank für die Antwort! Hier noch die Liste der zu bestellenden AKs: Wir werden den Dhh auf IHC/IHC-P testen sowie in einem zweiten Durchgang auch noch alle bestellten Aks auf Eignung für die Flow Cytometry. Bislang scheint nur Suppressor of Fused für letzteres erprobt zu sein; vielleicht koennen Sie ja nochmal diesbezueglich in der internen Datenbank nachschauen? Vielen Dank! |
|||||||||
ANSWER: |
Vielen Dank für Ihre Anfrage. In der Tat konnte ich in unserer Datenbank nichts darüber finden, dass die genannten Antikörper nicht auch für die Durchflußzytometrie geeignet sein könnten. Ich habe Ihnen daher die entsprechenden Gutscheincodes ausgestellt. Die Codes werden gültig, sobald Sie uns ein Abreview für den jeweiligen Antikörper über den Test in der Durchflußzytometrie eingereicht haben. ab92611: 100%ABR-T7THU ab26056: 100%ABR-T7THX ab53715: 100%ABR-T7THN ab72130: 100%ABR-T7THY Ablaufdatum jeweils 14.05.2012 (Wie zuvor: Die Bedingungen zu unserem 100% Abreview Rabatt können Sie unter dem folgenden Link nachlesen: http://www.abcam.com/collaborationdiscount) Ich habe Ihre Bestellung an Frau Stricker aus dem Kundendienst weitergeleitet. Sollte sie noch weitere Informationen benötigen, wird sie sich noch einmal bei Ihnen melden. Wir freuen uns auf alle Ihre Abreviews, egal ob die Ergebnisse positiv oder negativ sind und wünschen Ihnen viel Glück für Ihre Forschung. |
||||||||
|
|||||||||
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Anti-Gli2 antibody - ChIP Grade (ab26056) at 1 µg/ml (in 5% skim milk / PBS buffer) + Jurkat cell lysate 10ug.
Secondary
HRP conjugated anti-Rabbit IgG at 1/50000 dilution
Predicted band size : 133 kDa
Gel concentration: 10%
Ab26056 at 4-8 ug/ml staining paraffin embedded human heart tissue by Immunohistochemistry. Arrows indicate positively labelled myocardial cells. Magnification: x400.
Ab26056 at 4-8 ug/ml staining paraffin embedded human intestine tissue by Immunohistochemistry. Arrows indicate positively labelled epithelial cells of intestinal glands. Magnification: x400.
7
Call 01223 696 000 or contact us