Overview

  • Product nameAnti-Gli2 antibody - ChIP Grade
    See all Gli2 primary antibodies
  • Description
    Rabbit polyclonal to Gli2 - ChIP Grade
  • SpecificityAlthough some of our customers have had good results in Mouse (74% homology with the immunogen) and IHC, we do not batch test this antibody in this application and species. Due to the polyclonal nature of this antibody, some lots may work in Mouse and/or IHC but we cannot guarantee this.
  • Tested applicationsSuitable for: WB, ICC/IF, ICC, EMSA, ChIPmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to Human Gli2.
    Sequence:

    RNDVHLRTPLLKENGDSEAGTEPGGPESTEASSTSQAVEDCLHVRAIKTE


    Database link: P10070
    (Peptide available as ab130900)

  • Positive control
    • Jurkat cell lysate for Western blot, Human heart and human intestine tissue for Immunohistochemistry.

Properties

Applications

Our Abpromise guarantee covers the use of ab26056 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 4 µg/ml. Predicted molecular weight: 133 kDa.Can be blocked with Gli2 peptide (ab130900).

Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

ICC/IF Use at an assay dependent concentration.
ICC 1/200. See Abreview
EMSA Use a concentration of 1 µg/ml.
ChIP Use at an assay dependent concentration.

Target

  • FunctionActs as a transcriptional activator. May play a role during embryogenesis. Binds to the DNA sequence 5'-GAACCACCCA-3' which is part of the TRE-2S regulatory element that augments the Tax-dependent enhancer of human T-cell leukemia virus type 1. Implicated in the transduction of SHH signal.
  • Involvement in diseaseDefects in GLI2 are the cause of holoprosencephaly type 9 (HPE9) [MIM:610829]; also called pituitary anomalies with holoprosencephaly-like features. The primary features of this disease include defective anterior pituitary formation and pan-hypopituitarism, with or without overt forebrain cleavage abnormalities, and holoprosencephaly-like midfacial hypoplasia. Holoprosencephaly is the most common structural anomaly of the brain, in which the developing forebrain fails to correctly separate into right and left hemispheres. Holoprosencephaly is genetically heterogeneous and associated with several distinct facies and phenotypic variability.
  • Sequence similaritiesBelongs to the GLI C2H2-type zinc-finger protein family.
    Contains 5 C2H2-type zinc fingers.
  • Post-translational
    modifications
    Phosphorylated in vitro by ULK3.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • CJS antibody
    • Gli 2 antibody
    • GLI family zinc finger 2 antibody
    • GLI Kruppel family member GLI2 antibody
    • GLI2 antibody
    • GLI2_HUMAN antibody
    • Glioma associated oncogene family zinc finger antibody
    • HPE9 antibody
    • Oncogene GLI2 antibody
    • PHS2 antibody
    • Tax helper protein 1 antibody
    • Tax helper protein 2 antibody
    • Tax helper protein antibody
    • Tax responsive element 2 holding protein antibody
    • Tax responsive element 25 bp sequence binding protein antibody
    • THP antibody
    • THP1 antibody
    • THP2 antibody
    • Zinc finger protein GLI2 antibody
    see all

Anti-Gli2 antibody - ChIP Grade images

  • Anti-Gli2 antibody - ChIP Grade (ab26056) at 1 µg/ml (in 5% skim milk / PBS buffer) + Jurkat cell lysate 10ug.

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 133 kDa
    Gel concentration: 10%
  • All lanes : Anti-Gli2 antibody - ChIP Grade (ab26056) at 2.5 µg/ml

    Lane 1 : Jurkat cell lysate
    Lane 2 : Jurkat cell lysate with blocking peptide at 4 µg/ml

    Lysates/proteins at 25 µg per lane.


    Predicted band size : 133 kDa

References for Anti-Gli2 antibody - ChIP Grade (ab26056)

This product has been referenced in:
  • Chen Q  et al. Down-regulation of Gli transcription factor leads to the inhibition of migration and invasion of ovarian cancer cells via integrin ß4-mediated FAK signaling. PLoS One 9:e88386 (2014). WB, ICC/IF ; Human . Read more (PubMed: 24533083) »
  • Shahi MH  et al. Blocking Signaling at the Level of GLI Regulates Downstream Gene Expression and Inhibits Proliferation of Canine Osteosarcoma Cells. PLoS One 9:e96593 (2014). WB ; Dog . Read more (PubMed: 24810746) »

See all 24 Publications for this product

Product Wall

Application Western blot
Sample Human Cell lysate - whole cell (293)
Gel Running Conditions Reduced Denaturing
Loading amount 10 µg
Specification 293
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 25°C
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Submitted Aug 26 2016

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (Liver)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Dako K8004 or K8005, TRS High or low pH
Permeabilization No
Specification Liver
Fixative Formaldehyde
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Submitted Apr 27 2016

Application Western blot
Sample Human Cell lysate - whole cell (Human prostate cancer cell)
Gel Running Conditions Reduced Denaturing (10%)
Loading amount 30 µg
Specification Human prostate cancer cell
Blocking step Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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Submitted Sep 17 2015

Application Western blot
Loading amount 30 µg
Gel Running Conditions Reduced Denaturing (10% SDS PAGE)
Sample Mouse Tissue lysate - whole (Liver)
Specification Liver
Blocking step Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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Submitted Jul 23 2014

Based on the sequence of the immunizing peptide used to raise this antibody, this antibody will recognize the N-terminus region of isoform-5 (168kD), isoform-4 (86kD), isoform-3 (88kD), isoform-2 (132kD) and isoform-1 (133kD) of this human protein (htt...

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Based on the sequence of the immunizing peptide, this antibody is 100% homologous to all 5 isoforms of GLI2 from this reviewed UniProt entry (http://www.uniprot.org/uniprot/P10070).

Isoform 5 (168kD)
isoform alpha (133kD)
isoform be...

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Thank you for contacting us.

As I mentioned in our conversation, I think you are close to seeing a good result with the nuclear lysates. The adjustments we discussed (incubating stock antibody and peptide together in a small volume before d...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Mouse Cell (Intestine)
Specification Intestine
Fixative Paraformaldehyde
Permeabilization Yes - .01% triton x-100
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
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Submitted May 16 2012

Thank you for contacting us.

As you have requested, this blocking peptide has now been made availbale on our catalog. OurGli2 peptide is catalog number AB130900 and may be located at the following page:

http://www.abcam.com/Gli2...

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Thank you for contacting us and for bringing this to our attention.

After review of the materials for this product I have found that the immunogen sequence location provided was indeed incorrect. The immunogen was created from the area aa ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"