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Synthetic peptide corresponding to Human Gli2.
Our Abpromise guarantee covers the use of ab26056 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 - 1.25 µg/ml. Predicted molecular weight: 133 kDa.Can be blocked with Gli2 peptide (ab130900). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.|
|IHC-P||Use at an assay dependent concentration.|
|ICC/IF||Use at an assay dependent concentration.|
|ICC||1/200. See Abreview|
|EMSA||Use a concentration of 1 µg/ml.|
|IHC-Fr||1/100. See Abreview|
|ChIP||Use at an assay dependent dilution.|
ab26056 staining Gli2 in murine intestine by Immunohistochemistry-PFA fixed. Tissue was fixed in paraformaldehyde, permeabilized using 0.01% Triton X-100, blocked with 5% BSA for 1 hour at 23°C and then incubated with ab26056 at a 1/300 dilution for 8 hours at 20°C. The secondary used was an undiluted Alexa-Fluor 488 conjugated donkey anti-rabbit polyclonal.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human heart tissue labelling Gli2 with ab26056 at a concentration of 4-8µg/ml. Arrows indicate positively labelled myocardial cells. Magnification: x400.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human intestine tissue labelling Gli2 with ab26056 at a concentration of 4-8µg/ml. Arrows indicate positively labelled epithelial cells of intestinal glands. Magnification: x400.
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