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Synthetic peptide: RNDVHLRTPL LKENGDSEAG TEPGGPESTE ASSTSQAVED CLHVRAIKTE, corresponding to amino acids 600-649 of Human Gli2 (Peptide available as ab111657).
Our Abpromise guarantee covers the use of ab26056 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 - 1.25 µg/ml. Predicted molecular weight: 133 kDa.Can be blocked with Human Gli2 peptide (ab111657). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.|
|IHC-P||Use at an assay dependent concentration.|
|ICC/IF||Use at an assay dependent concentration.|
|ICC||1/200. See Abreview|
|EMSA||Use a concentration of 1 µg/ml.|
|IHC-Fr||1/100. See Abreview|
|ChIP||Use at an assay dependent dilution.|
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human heart tissue labelling Gli2 with ab26056 at a concentration of 4-8µg/ml. Arrows indicate positively labelled myocardial cells. Magnification: x400.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human intestine tissue labelling Gli2 with ab26056 at a concentration of 4-8µg/ml. Arrows indicate positively labelled epithelial cells of intestinal glands. Magnification: x400.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"