Overview

  • Product nameAnti-Gli2 antibodySee all Gli2 primary antibodies ...
  • Description
    Rabbit polyclonal to Gli2
  • Specificityab7181 is expected to cross-react with all 4 splice variants of Human Gli as the immunogen sequence is present in all 4. Tested by Western blotting on human lung, gives low background, but no band was detected. We welcome feedback on this antibody.
  • Tested applicationsIHC-P, IHC-Frmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide:

    RSKVKTEPEGLRPAS-C

    conjugated to KLH, corresponding to amino acids 46-60 of Human Gli2.

Properties

Applications

Our Abpromise guarantee covers the use of ab7181 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P
IHC-Fr
  • Application notesIHC-P: 1/200. (PMID 19107131)
    IHC-Fr: 1/100.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionActs as a transcriptional activator. May play a role during embryogenesis. Binds to the DNA sequence 5'-GAACCACCCA-3' which is part of the TRE-2S regulatory element that augments the Tax-dependent enhancer of human T-cell leukemia virus type 1. Implicated in the transduction of SHH signal.
    • Involvement in diseaseDefects in GLI2 are the cause of holoprosencephaly type 9 (HPE9) [MIM:610829]; also called pituitary anomalies with holoprosencephaly-like features. The primary features of this disease include defective anterior pituitary formation and pan-hypopituitarism, with or without overt forebrain cleavage abnormalities, and holoprosencephaly-like midfacial hypoplasia. Holoprosencephaly is the most common structural anomaly of the brain, in which the developing forebrain fails to correctly separate into right and left hemispheres. Holoprosencephaly is genetically heterogeneous and associated with several distinct facies and phenotypic variability.
    • Sequence similaritiesBelongs to the GLI C2H2-type zinc-finger protein family.
      Contains 5 C2H2-type zinc fingers.
    • Post-translational
      modifications
      Phosphorylated in vitro by ULK3.
    • Cellular localizationNucleus.
    • Information by UniProt
    • Database links
    • Alternative names
      • Gli 2 antibody
      • GLI family zinc finger 2 antibody
      • GLI kruppel family member 2 antibody
      • GLI Kruppel family member GLI2 antibody
      • GLI2 antibody
      • GLI2_HUMAN antibody
      • Glioma associated oncogene family zinc finger antibody
      • HPE9 antibody
      • Oncogene GLI2 antibody
      • Tax helper protein antibody
      • Tax helper protein 1 antibody
      • Tax helper protein 2 antibody
      • Tax responsive element 2 holding protein antibody
      • Tax responsive element 25 bp sequence binding protein antibody
      • THP 2 antibody
      • THP antibody
      • THP1 antibody
      • THP2 antibody
      • Zinc finger protein GLI2 antibody
      see all

    References for Anti-Gli2 antibody (ab7181)

    This product has been referenced in:
    • Im S  et al. Hedgehog related protein expression in breast cancer: gli-2 is associated with poor overall survival. Korean J Pathol 47:116-23 (2013). IHC-P ; Human . Read more (PubMed: 23667370) »
    • Brunner M  et al. Expression of hedgehog signaling molecules in Merkel cell carcinoma. Head Neck 32:333-40 (2010). IHC-P ; Human . Read more (PubMed: 19644931) »

    See all 3 Publications for this product

    Product Wall

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunohistochemistry (Frozen sections)
    Sample Mouse Tissue sections (E12.5 Whole brain section)
    Specification E12.5 Whole brain section
    Fixative Paraformaldehyde
    Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5%
    Username

    Dr. Dean Griffiths

    Verified customer

    Submitted Jan 04 2007

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"