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ab7195 has been referenced in 7 publications.
Publishing research using ab7195? Please let us know so that we can cite the reference in this datasheet
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All lanes : Anti-Gli2 antibody (ab7195)
Lane 1 : Mouse brain whole cell lysate
Lane 2 : Mouse brain whole cell lysate with Immunizing peptide
Lysates/proteins at 25 µg per lane.
Secondary
IRDye™800
conjugated Gt-a-Rabbit IgG (1/10000)
Observed band size : 190 kDa (why is the actual band size different from the predicted?)
ab7195 at 10 µg/ml staining mouse testis.
ab7195 at 10 µg/ml staining mouse brain.
developed using the ECL technique
Observed band size : 160 kDa (why is the actual band size different from the predicted?)
Exposure time : 2 minutes
Gli2 protein levels were determined by WB in 2 adult mice in either cerebral cortex (CC) or in cerebellum (CB). Nuclear lysis buffer has to be used otherwise no bands can be detected. BSA blocking is better than milk.20µg lysate added per lane.ab7195 used at a 1/1000 dilution.Secondary used was an HRP conjugated goat anti-rabbit polyclonal used at a 1/10000 dilution.
Image courtesy of an anonymous Abreview.
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