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Overview

Properties

Applications

Our Abpromise guarantee covers the use of ab7195 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
ELISA
WB
IHC-P
IHC-Fr
  • Application notesELISA: 1/15000 - 1/60000.
    IHC-P: Use at a concentration of 2 - 20 µg/ml.
    IHC-Fr: Use at an assay dependent dilution ~(PMID 17971852).
    WB: 1/500 - 1/2000.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    • Target

    • FunctionActs as a transcriptional activator. May play a role during embryogenesis. Binds to the DNA sequence 5'-GAACCACCCA-3' which is part of the TRE-2S regulatory element that augments the Tax-dependent enhancer of human T-cell leukemia virus type 1. Implicated in the transduction of SHH signal.
    • Involvement in diseaseDefects in GLI2 are the cause of holoprosencephaly type 9 (HPE9) [MIM:610829]; also called pituitary anomalies with holoprosencephaly-like features. The primary features of this disease include defective anterior pituitary formation and pan-hypopituitarism, with or without overt forebrain cleavage abnormalities, and holoprosencephaly-like midfacial hypoplasia. Holoprosencephaly is the most common structural anomaly of the brain, in which the developing forebrain fails to correctly separate into right and left hemispheres. Holoprosencephaly is genetically heterogeneous and associated with several distinct facies and phenotypic variability.
    • Sequence similaritiesBelongs to the GLI C2H2-type zinc-finger protein family.
      Contains 5 C2H2-type zinc fingers.
    • Post-translational
      modifications      Phosphorylated in vitro by ULK3.
    • Cellular localizationNucleus.

      • Target information above from: UniProt accession P10070 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links
      • Alternative names
          Gli 2 antibodyGLI family zinc finger 2 antibodyGLI kruppel family member 2 antibody
          GLI Kruppel family member GLI2 antibodyGLI2 antibodyGLI2_HUMAN antibodyOncogene GLI2 antibodyTax helper protein antibodyTax helper protein 2 antibodyTax responsive element 2 holding protein antibodyTax responsive element 25 bp sequence binding protein antibodyTHP 2 antibodyTHP antibodyTHP2 antibodyZinc finger protein GLI2 antibody
        see all

      Anti-Gli2 antibody images

      • Western blot - Gli2 antibody (ab7195)
        Western blot - Gli2 antibody (ab7195)
        All lanes : Anti-Gli2 antibody (ab7195)

        Lane 1 : Mouse brain whole cell lysate
        Lane 2 : Mouse brain whole cell lysate with Immunizing peptide

        Lysates/proteins at 25 µg per lane.

        Secondary
        IRDye™800 conjugated Gt-a-Rabbit IgG (1/10000)

        Observed band size : 190 kDa (why is the actual band size different from the predicted?)
      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Gli2 antibody (ab7195)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Gli2 antibody (ab7195)

        ab7195 at 10 µg/ml staining mouse testis.

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Gli2 antibody (ab7195)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Gli2 antibody (ab7195)

        ab7195 at 10 µg/ml staining mouse brain.

      • Western blot - Anti-Gli2 antibody (ab7195)
        Western blot - Anti-Gli2 antibody (ab7195)

        developed using the ECL technique

        Observed band size : 160 kDa (why is the actual band size different from the predicted?)


        Exposure time : 2 minutes

        Image courtesy of an anonymous Abreview.

        See Abreview

      References for Anti-Gli2 antibody (ab7195)

      This product has been referenced in:
      • Zhang Z  et al. Fuz Regulates Craniofacial Development through Tissue Specific Responses to Signaling Factors. PLoS One 6:e24608 (2011). IHC-P ; Mouse . Read more (PubMed: 21935430) »
      • Youssef KK  et al. Identification of the cell lineage at the origin of basal cell carcinoma. Nat Cell Biol 12:299-305 (2010). Read more (PubMed: 20154679) »

      See all 7 Publications for this product

      Publishing research using ab7195 ? Please let us know so that we can cite the reference in this datasheet

      Product Wall

      Submit an Abreview for ab7195 Submit a Question for ab7195

      Displaying 1 - 10 of 10 results for Abreviews and Q&A

      10
      Votes: Highest First

      Could you answer a few more questions.   1)      When you do antigen retrieval for ab85898, for how long do you boil in citric buffer? 2)      When you incubate with ab85898 antibodies, what solution do you use for mouse tissues (IHC-P)? Is it the same...

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      Thank you for contacting us. I have tried to answer your questions below and have attached our IHC-P protocol for your reference.

      1) When you do antigen retrieval for ab85898, for how long do you boil in citric buffer?We recommend boiling in a ...

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      Hi
      I am back today and think I will take your kind offer of trying as replacement the antibody ab26056 as it at least means I am coming at this problem from a fresh perspective. I have tired ab7195 so many times now and although it may indeed be that...

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      Thank you for getting back to me. I think it does seem sensible to try ab26056 in order to see if any improvement is made. It may be that you will still need to increase the amount of lysate loaded and/or the antibody concentration but see how you get on....

      Read More

      Thanks for your this, this is very helpful. The other antibody does look good, i wish i noticed it before. There is a clear band at 1:1000 conc with just 10ug of mouse cell lysate. My current antibody has been shown to need 25ug lysate i believe and the b...

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      That's no problem. Take your time considering your options and how you would like to proceed. Antibody ab26056 is indeed a very popular antibody against Gli2. I was not sure if you were interested in this antibody or not as the immunogen is taken from res...

      Read More

      Hi

      The Gli2 protein actually comes in two forms; the 165 kDA (actually larger due to post translational mods) and 75kDA cleaved form which acts as a weak pathway repressor (Gli3 is the main repressor). There is a paper by Baolin Wang (Pan et al...

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      Sorry for the delayin getting back to you. We have been closedover the Easter weekendperiod.

      Thank you for getting back to me with that information.I can well understand your point of view. My concern is that, as you say, our antibody is suppose...

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      Dear
      Thank you for getting back to me so fast, I will try and answer your questions as best as I can.

      Fatty Acid Synthase is 270 kDA and looking at image1 attached, which is what I am seeing (primary FAS (A-5)) against my ladders which is ...

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      Thank you for clarifying that. I now understand much more fully the results that you have been obtaining.

      I can see that you are clearly detecting the FAS protein, as well as a band at ˜75 kDa with the other anti-Gli2 antibody. May I ask, why ar...

      Read More

      In response to your email please find information requested below and attached. Many Thanks
      1) Abcam product code

      ab7195

      2) Abcam order reference number or product batch number

      3) Description of the problem

      Read More

      Thank you for taking time to complete our questionnaire. The details provided will enable us to investigate this case and will provide us with vital information for monitoring product quality.

      Having reviewed the information provided, I have a ...

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      Phone call reporting problems in using Anti-Gli2 antibody ab7195 in western blotting.

      Thank you for calling us and for alerting us to the problem you are experiencing with our product. We take product complaints very seriously, and investigate every product that we feel may not be performing correctly.

      As we discussed on the pho...

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      Western blot abreview for Anti-Gli2 antibody

       Good
      Abreviews
      Application Western blot
      Sample Mouse Tissue lysate - nuclear (brain tissue)
      Loading amount 20 µg
      Specification brain tissue
      Gel Running Conditions Reduced Denaturing (7%)
      Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 22°C
      Read More
      abreview image
      Username

      Verified customer

      Submitted Dec 15 2011

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Gli2 antibody

       Excellent
      Abreviews
      Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
      Sample Mouse Tissue sections (cerebral cortex)
      Specification cerebral cortex
      Fixative Paraformaldehyde
      Antigen retrieval step Heat mediated - Buffer/Enzyme Used: 0.01 M Tris HCL, pH 10
      Permeabilization Yes - 0.3% Triton
      Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 22°C
      Read More
      abreview image
      Username

      Verified customer

      Submitted Aug 02 2011

      Western blot abreview for Anti-Gli2 antibody

       Average
      Abreviews
      Application Western blot
      Sample Mouse Cell lysate - whole cell (NIH3T3)
      Specification NIH3T3
      Blocking step Milk as blocking agent for 15 hour(s) and 0 minute(s) · Concentration: 5%
      Read More
      Username

      Verified customer

      Submitted Nov 13 2006

      Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"