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Western blot - Gli3 antibody (ab6050)
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Immunohistochemistry (Frozen sections) - Gli3 antibody (ab6050)
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Gli3 antibody (ab6050)
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Product Name
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Gli3 antibody
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See all Gli3 antibodies (3)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to Gli3
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Immunogen
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Synthetic peptide: NEDESPGQTYHRERRNA-C conjugated to KLH, corresponding to amino acids 41-57 of Human GLI3.
BLAST 'NEDESPGQTYHRERRNA-C' with  or 
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Reacts with
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Ms
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Predicted to react
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Hu, Chk, Xl
(Due to sequence homology)
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Tested applications
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IHC-Fr
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Abreviews
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Application notes
(see key)
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Recommended dilutions
IHC-Fr: 1/100. Please see Abreview for method details.
WB: It was not possible to detect Gli3 immunoreactivity by WB in the lysates we tested in house. However this maybe due to low expression/abundance of Gli3 in these tissue lysates. Gli3 expression is likely to be developmentally regulated and induced, making it difficult to succesfully detect in whole tissue homogenates. However, the staining on the WB showed relatively low background and minimal cross reactivity in whole homogenates.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Cellular localization
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Nuclear
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Research areas
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Stem Cells >> Signaling Pathways >> Hedgehog >> Nuclear
Neuroscience >> Neurology process >> Neural Signal Transduction
Chromatin and Nuclear Signaling >> Transcription >> Domain Families >> Zinc Finger
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Relevance
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Gli3 belong to the GLI C2H2-type zinc-finger protein family and contains five C2H2-type zinc fingers. Gli3 is very important for normal limb and brain development and is implicated in the transduction of Shh signal. Gli3 is also expressed in a wide variety of normal adult tissues, including lung, colon, spleen, placenta, testis, and myometrium. Defects in GLI3 are the cause of Greig cephalo-poly-syndactyly syndrome (GCPS); an autosomal dominant disorder affecting limb and craniofacial development.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: 0.01% Sodium Azide
Constituents: 0.15M Sodium chloride, 0.02M Potassium phosphate. pH 7.2 Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Form
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Liquid
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Concentration
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1.210 mg/ml
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this Gli3 antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for Gli3 antibody images, references, products related to ab6050 and other tools.
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Gli3 antibody images:
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 Western blot - Gli3 antibody (ab6050)
Western blot for Gli3 antibody (ab6050) at 1/500 tested on the following tissue lysates: Lane 1 : Human Brain
Lane 2: Human Lung
Lane 3: Human Spleen
Lane 4: Mouse Brain
Lane 5: Mouse Lung Secondary ab: Goat anti-rabbit IgG ab6721 (1/5000)
Exposure time: 3 minutes
Expected molecular weight: 2 isoforms of Gli3 exist, one is the full length 170-190kDa and the other is a truncated isoform at ~80kDa.
Cell lysates were loaded at 20µg per lane. NB: This image shows the truncated isoform of Gli3 is detected by ab6050 in human lung (we do not know the identity of the 120-130kDa band). It was not possible to detect Gli3 immunoreactivity by WB in the other lysates tested. This is likely due to low expression/abundance of Gli3 in these tissue lysates. Furthermore, Gli3 expression is likely to be developmentally regulated and induced, making it difficult to succesfully detect in whole tissue homogenates. The staining on the WB shows low background and minimal cross reactivity in whole homogenates. The 50kDa band is likely due to non-specific binding of secondary antibody.
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Immunohistochemistry (Frozen sections) - Gli3 antibody (ab6050)
Gil3 expression in the radial glia of the developing mouse neocortex.
ab6050 Rabbit polyclonal to Gil3 on Frozen sections of mouse E12.5 whole brain, showing staing of the radial glia, of the developing neocortex. Sections were paraformaldehyde fixed, prior to heat mediated antigen retrieval in citric acid, and 14 hours incubation with ab6050 (1/100).
This image is courtesy of an Abreview submitted by Dr Dean Griffiths
See Abreview
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Gli3 antibody (ab6050)
ab6050 at 0.625 µg/ml staining human glioblastoma.
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Search PubMed (MEDLINE) for references to Gli3
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Gli3 antibody - more information
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Customer reviews (feedback) regarding Gli3 antibody |
Customer FAQs regarding Gli3 antibody |
Protocols for Gli3 antibody |
Price and availability of products related to Gli3 antibody |
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Gli3 antibody - related products:
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Gli3 antibody - other tools:
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Contact Abcam with a Technical Enquiry about Gli3 antibody |
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