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ab69917 |
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Read our guarantee »Products:Epigenetics and Nuclear Signaling >> Transcription >> Domain Families >> Zinc Finger
Anti-Gli3 antibody
See all Gli3 products (5) ...
Rabbit polyclonal to Gli3
Reacts with
Mouse, Human
Predicted to work with
Rat, Chicken, Chimpanzee, Rhesus monkey
Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human Gli3.
(Peptide available as ab69917.)
This antibody gave a positive signal in the following Lysates: Mouse Pancreas Tissue, Mouse Liver Tissue, MEF1 Whole Cell, TE 671 Whole Cell, RAW 264.7 Whole Cell
Liquid
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration information loading...
Immunogen affinity purified
Polyclonal
IgG
Stem Cells >> Signaling Pathways >> Hedgehog >> Nuclear
Neuroscience >> Neurology process >> Neural Signal Transduction
Epigenetics and Nuclear Signaling >> Transcription >> Domain Families >> Zinc Finger
Western blot - Gli3 antibody (ab69838)
(enlarge)
Our Abpromise guarantee covers the use of ab69838 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use a concentration of 1 µg/mlDetects a band of approximately 145 kDa (predicted molecular weight: 160 kDa).
Has a dual function as a transcriptional activator and a repressor of the sonic hedgehog (Shh) pathway, and plays a role in limb development. The full-length GLI3 form (GLI3FL) after phosphorylation and nuclear translocation, acts as an activator (GLI3A) while GLI3R, its C-terminally truncated form, acts as a repressor. A proper balance between the GLI3 activator and the repressor GLI3R, rather than the repressor gradient itself or the activator/repressor ratio gradient, specifies limb digit number and identity. In concert with TRPS1, plays a role in regulating the size of the zone of distal chondrocytes, in restricting the zone of PTHLH expression in distal cells and in activating chondrocyte proliferation. Binds to the minimal GLI-consensus sequence 5'-GGGTGGTC-3'.
Is expressed in a wide variety of normal adult tissues, including lung, colon, spleen, placenta, testis, and myometrium.
Defects in GLI3 are the cause of Greig cephalo-poly-syndactyly syndrome (GCPS) [MIM:175700]. GCPS is an autosomal dominant disorder affecting limb and craniofacial development. It is characterized by pre- and postaxial polydactyly, syndactyly of fingers and toes, macrocephaly and hypertelorism.
Defects in GLI3 are a cause of Pallister-Hall syndrome (PHS) [MIM:146510]. PHS is characterized by a wide range of clinical manifestations. It mainly associates central or postaxial polydactyly, syndactyly, and hypothalamic hamartoma. Malformations are frequent in the viscera, e.g. anal atresia, bifid uvula, congenital heart malformations, pulmonary or renal dysplasia. It is an autosomal dominant disorder.
Defects in GLI3 are a cause of type A1/B postaxial polydactyly (PAPA1/PAPB) [MIM:174200, 603596]. PAPA in humans is an autosomal dominant trait characterized by an extra digit in the ulnar and/or fibular side of the upper and/or lower extremities. The extra digit is well formed and articulates with the fifth, or extra, metacarpal/metatarsal, and thus it is usually functional.
Defects in GLI3 are a cause of polydactyly preaxial type 4 (POP4) [MIM:174700]. Polydactyly preaxial type 4 (i.e., polydactyly on the radial/tibial side of the hand/foot) covers a heterogeneous group of entities. In preaxial polydactyly type IV, the thumb shows only the mildest degree of duplication, and syndactyly of various degrees affects fingers 3 and 4.
Defects in GLI3 are the cause of acrocallosal syndrome (ACS) [MIM:200990]; also abbreviated ACLS. ACS is characterized by postaxial polydactyly, hallux duplication, macrocephaly, and absence of the corpus callosum, usually with severe developmental delay.
Belongs to the GLI C2H2-type zinc-finger protein family.
Contains 5 C2H2-type zinc fingers.
Phosphorylated on multiple sites by protein kinase A (PKA) and phosphorylation by PKA primes further phosphorylation by CK1 and GSK3. Phosphorylation is essential for its proteolytic processing.
Transcriptional repressor GLI3R, a C-terminally truncated form, is generated from the full-length GLI3 protein (GLI3FL/GLI3-190) through proteolytic processing. This process requires PKA-primed phosphorylation of GLI3, ubiquitination of GLI3 and the presence of BTRC. GLI3FL is complexed with SUFU in the cytoplasm and is maintained in a neutral state. Without the Hh signal, the SUFU-GLI3 complex is recruited to cilia, leading to the efficient processing of GLI3FL into GLI3R. GLI3R formation leads to its dissociation from SUFU, allowing it to translocate into the nucleus, and repress Hh target genes. When Hh signaling is initiated, SUFU dissociates from GLI3FL and this has two consequences. First, GLI3R production is halted. Second, free GLI3FL translocates to the nucleus, where it is phosphorylated, destabilized, and converted to a transcriptional activator (GLI3A). Phosphorylated in vitro by ULK3.
Nucleus. Cytoplasm. Cell projection > cilium. GLI3FL is localized predominantly in the cytoplasm while GLI3R resides mainly in the nucleus. Ciliary accumulation requires the presence of KIF7 and SMO. Translocation to the nucleus is promoted by interaction with ZIC1.
Target information above from: UniProt accessionP10071
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Western blot - Gli3 antibody (ab69838)

All lanes : Anti-Gli3 antibody (ab69838) at 1 µg/ml
Lane 1 : Pancreas (Mouse) Tissue Lysate
Lane 2 : Liver (Mouse) Tissue Lysate
Lane 3 : MEF1 (Mouse embryonic fibroblast cell line) Whole Cell Lysate
Lane 4 : TE 671 (Human Rhabdomyosarcoma) Whole Cell Lysate
Lane 5 : RAW 264.7 (Mouse leukaemic monocyte macrophage cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size : 160 kDa
Observed band size : 145 kDa (why is the actual band size different from the predicted?)
Additional bands at : 110 kDa,55 kDa,75 kDa. We are unsure as to the identity of these extra bands.
ab69838 has not yet been referenced specifically in any publications.
Publishing research using ab69838? Please let us know so that we can cite the reference in this datasheet
Concentration of lot no. is
Concentration not available for this lot.
Find concentration of your lot:

All lanes : Anti-Gli3 antibody (ab69838) at 1 µg/ml
Lane 1 : Pancreas (Mouse) Tissue Lysate
Lane 2 : Liver (Mouse) Tissue Lysate
Lane 3 : MEF1 (Mouse embryonic fibroblast cell line) Whole Cell Lysate
Lane 4 : TE 671 (Human Rhabdomyosarcoma) Whole Cell Lysate
Lane 5 : RAW 264.7 (Mouse leukaemic monocyte macrophage cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size : 160 kDa
Observed band size : 145 kDa (why is the actual band size different from the predicted?)
Additional bands at : 110 kDa,55 kDa,75 kDa. We are unsure as to the identity of these extra bands.

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