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Western blot - Gli3 antibody (ab69838)
(enlarge)
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Product Name
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Gli3 antibody
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See all Gli3 antibodies (3)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to Gli3
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Immunogen
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Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human Gli3. (Peptide available as ab69917.)
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Reacts with
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Hu, Ms
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Predicted to react
(species key)
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Chk, Chmp, RMk
(Due to sequence homology)
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Tested applications
(see key)
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WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
WB: Use at a concentration of 1 µg/ml. Detects a band of approximately 145 kDa (predicted molecular weight: 160 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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This antibody gave a positive signal in the following Lysates:
Mouse Pancreas Tissue, Mouse Liver Tissue, MEF1 Whole Cell, TE 671 Whole Cell, RAW 264.7 Whole Cell
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Cellular localization
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Nuclear
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Research areas
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Stem Cells >> Signaling Pathways >> Hedgehog >> Nuclear
Neuroscience >> Neurology process >> Neural Signal Transduction
Chromatin and Nuclear Signaling >> Transcription >> Domain Families >> Zinc Finger
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Relevance
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Gli3 belong to the GLI C2H2-type zinc-finger protein family and contains five C2H2-type zinc fingers. Gli3 is very important for normal limb and brain development and is implicated in the transduction of Shh signal. Gli3 is also expressed in a wide variety of normal adult tissues, including lung, colon, spleen, placenta, testis, and myometrium. Defects in GLI3 are the cause of Greig cephalo-poly-syndactyly syndrome (GCPS); an autosomal dominant disorder affecting limb and craniofacial development.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4 Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Form
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Liquid
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Concentration
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Batch dependent within range: 0.10 - 0.30 mg/ml
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Storage instructions
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Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this Gli3 antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for Gli3 antibody images, references, products related to ab69838 and other tools.
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Gli3 antibody images:
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 Western blot - Gli3 antibody (ab69838)
All lanes : Gli3 antibody (ab69838) at 1 µg/ml
Lane 1 : Pancreas (Mouse) Tissue Lysate
Lane 2 : Liver (Mouse) Tissue Lysate
Lane 3 : MEF1 (Mouse embryonic fibroblast cell line) Whole Cell Lysate
Lane 4 : TE 671 (Human Rhabdomyosarcoma) Whole Cell Lysate
Lane 5 : RAW 264.7 (Mouse leukaemic monocyte macrophage cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size : 160 kDa
Observed band size : 145 kDa (why is the actual band size different from the predicted?)
Additional bands at : 110 kDa,55 kDa,75 kDa. We are unsure as to the identity of these extra bands.
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Search PubMed (MEDLINE) for references to Gli3
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Gli3 antibody - more information
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Customer reviews (feedback) regarding Gli3 antibody |
Customer FAQs regarding Gli3 antibody |
Protocols for Gli3 antibody |
Price and availability of products related to Gli3 antibody |
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Gli3 antibody - related products:
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Gli3 antibody - other tools:
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Contact Abcam with a Technical Enquiry about Gli3 antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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