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Western blot - Gli3 antibody (ab69838)
Western blot - Gli3 antibody (ab69838)
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Product Name 

Gli3 antibody

 

See all Gli3 antibodies (3)...

Product type 

Primary antibodies

Description 

Rabbit polyclonal to Gli3

Immunogen 

Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human Gli3.

(Peptide available as ab69917.)

Reacts with 

Hu, Ms

Predicted to react 
(species key)

Chk, Chmp, RMk
(Due to sequence homology)

Tested applications 
(see key)

WB


Abreviews 

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(see key)


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Application notes 
(see key)

Recommended dilutions
WB: Use at a concentration of 1 µg/ml. Detects a band of approximately 145 kDa (predicted molecular weight: 160 kDa).

Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Positive control 
(see definition)

This antibody gave a positive signal in the following Lysates:
Mouse Pancreas Tissue, Mouse Liver Tissue, MEF1 Whole Cell, TE 671 Whole Cell, RAW 264.7 Whole Cell

Cellular localization 

Nuclear

Research areas 

Stem Cells >> Signaling Pathways >> Hedgehog >> Nuclear
Neuroscience >> Neurology process >> Neural Signal Transduction
Chromatin and Nuclear Signaling >> Transcription >> Domain Families >> Zinc Finger

Relevance 

Gli3 belong to the GLI C2H2-type zinc-finger protein family and contains five C2H2-type zinc fingers. Gli3 is very important for normal limb and brain development and is implicated in the transduction of Shh signal. Gli3 is also expressed in a wide variety of normal adult tissues, including lung, colon, spleen, placenta, testis, and myometrium. Defects in GLI3 are the cause of Greig cephalo-poly-syndactyly syndrome (GCPS); an autosomal dominant disorder affecting limb and craniofacial development.

 

Alternative names Gli3 antibody (ab69838)

Database links 

The links below go to external sites and will open in a new browser window

Entrez Gene

    

2737    (Human)
14634    (Mouse)
140588    (Rat)

Omim

    

165240    (Human)

SwissProt

    

P10071    (Human)
Q61602    (Mouse)

Unigene

    

199338    (Human)
5098    (Mouse)
92397    (Rat)

Raised in 

Rabbit

Clonality 

Polyclonal

Isotype 

IgG

Purity 

Immunogen affinity purified

Storage buffer 

Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4

Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS

Form 

Liquid

Concentration 

Batch dependent within range: 0.10 - 0.30 mg/ml

Concentration of lot no.     is  

Concentration not available for this lot.

Find concentration of your lot :

Storage instructions 

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

 

At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this Gli3 antibody is on this datasheet. But please do contact us if you would like any reassurance!


See below for Gli3 antibody images, references, products related to ab69838 and other tools.

 

Gli3 antibody images:

Product image  Western blot - Gli3 antibody (ab69838)

Western blot - Gli3 antibody (ab69838)

All lanes : Gli3 antibody (ab69838) at 1 µg/ml

Lane 1 : Pancreas (Mouse) Tissue Lysate
Lane 2 : Liver (Mouse) Tissue Lysate
Lane 3 : MEF1 (Mouse embryonic fibroblast cell line) Whole Cell Lysate
Lane 4 : TE 671 (Human Rhabdomyosarcoma) Whole Cell Lysate
Lane 5 : RAW 264.7 (Mouse leukaemic monocyte macrophage cell line) Whole Cell Lysate

Lysates/proteins at 10 µg per lane.

Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

Performed under reducing conditions.

Predicted band size : 160 kDa
Observed band size : 145 kDa (why is the actual band size different from the predicted?)
Additional bands at : 110 kDa,55 kDa,75 kDa. We are unsure as to the identity of these extra bands.

 
 

Search PubMed (MEDLINE) for references to Gli3

 

Gli3 antibody - more information

Customer reviews (feedback) regarding Gli3 antibody

Customer FAQs regarding Gli3 antibody

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Price and availability of products related to Gli3 antibody

 

Gli3 antibody - related products:

Products for Western blot

Loading controls

Lysates

 

Immunizing Peptide for Gli3 antibody

ab69917

  

Gli3 peptide

 

Compatible Secondaries for Gli3 antibody

ab6564

  

Cy5 ® antibody

ab6717

  

FITC antibody

ab6720

  

Biotin antibody

ab6721

  

HRP antibody

ab6722

  

Alkaline Phosphatase antibody

ab6793

  

Texas Red ® antibody

ab7007

  

Phycoerythrin antibody

ab7019

  

Agarose antibody

ab27234

  

10nm Gold antibody

ab27235

  

5nm Gold antibody

ab27236

  

15nm Gold antibody

ab27237

  

20nm Gold antibody

ab27238

  

30nm Gold antibody

ab27239

  

10nm Gold antibody

ab27240

  

5nm Gold antibody

ab31193

  

Duck polyclonal to Rabbit IgG

ab60314

  

Chromeo™ 488 antibody

ab60315

  

Chromeo™ 494 antibody

ab60317

  

Chromeo™ 546 antibody

ab60319

  

Chromeo™ 642 antibody

 

Other Gli3 Antibodies

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Gli3 antibody - other tools:

Contact Abcam with a Technical Enquiry about Gli3 antibody

 

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