Loading...
You have changed your country from
to
. Please be aware that this will change the currency in the purchasing process.
Related products
- Secondary antibodies
Reassurance, Refunds & Replacements
If your product does not perform as described on this datasheet, we will refund or replace your product...
Read our guarantee »Overview
Product name
Anti-Glu-Glu tag antibody [Glu-Glu] - Affinity Matrix
See all Glu-Glu tag products (9) ...
Description
Mouse monoclonal [Glu-Glu] to Glu-Glu tag - Affinity Matrix
Specificity
This affinity Matrix can be used for immunopurification of Glu-Glu tagged fusion proteins from crude starting material.Binding: Tagged protein will bind to matrix in common physiologic buffers with pH in the range of 6.0-7.5, salt from 50-500 mM and in the presence of reasonable levels of detergent. Excess reducing agent should be avoided as the disulfide bridges holding antibody heavy and light chains may be compromised.Washing: After binding, washes with several bead volumes of buffer are recommended.Elution: Several options are available for elution. 1. SDS gel loading buffer may be applied directly to the beads in order to display all bound protein on a polyacrylamide gel/western. Note that gel loading buffer containing reducing agent will also release some antibody heavy and light chains (approx 25 and 50kD, respectively). 2. Competitive elution with epitope peptide. For epitope tag affinity matrices, prepare an elution buffer with epitope tag peptide at 400 ug/ml in 50 mM Tris-HCl (pH 7.5), 50 mM NaCl, 1 mM EDTA (pH 8.0). 3. Chemical elution. Elution by pH or chaotropic salts is also possible. For elution by pH, either 0.1 M glycine pH 2.8 or 40 mM diethyl-amine pH 11.0 may be used.
Tested applications
Immunogen
Synthetic peptide: CEEEEYMPME derived from the polyoma virus medium T antigen.
CEEEEYMPME
Epitope
ab24587 is extremely specific and recognizes either six amino acid sequence, EYMPME, or EFMPME.
Properties
Form
Liquid
Storage instructions
Store at +4°C.
Storage buffer
The slurry contains,
Preservative: 0.03% Thimerosal (merthiolate)
Constituents: PBS
Concentration
Concentration information loading...
Purity
Protein G purified
Purification notes
This antibody was purified using protein-G chromatography and was subsequently immobilized onto a Sepharose(TM) Fast Flow matrix.
Clonality
Monoclonal
Clone number
Glu-Glu
Isotype
IgG
Research areas
Applications
Show applications key
Our Abpromise guarantee covers the use of ab24587 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
AP: Use at an assay dependent dilution. Note: The matrix may be re-used several times. To strip column after use, wash with several bead volumes 0.1 M glycine pH 2.8 followed immediately by PBS containing 0.3% thimerosol or 1mM azide as preservative.
IP: Use at an assay dependent dilution.
Target
Alternative names
- EYMPME epitope tag antibody
- EYMPME epitope tag antibody
- EYMPME tag antibody
see all
References for Anti-Glu-Glu tag antibody [Glu-Glu] - Affinity Matrix (ab24587)
This product has been referenced in:
- Knuesel MT et al. The human CDK8 subcomplex is a histone kinase that requires Med12 for activity and can function independently of mediator. Mol Cell Biol 29:650-61 (2009).Read more (PubMed: 19047373) »
See 1 publication for this product
Publishing research using ab24587? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"